Plant Foods for Human Nutrition

, Volume 65, Issue 3, pp 284–289 | Cite as

Protective Role of Three Vegetable Peels in Alloxan Induced Diabetes Mellitus in Male Mice



The hitherto unknown glucose regulating role of three vegetable peels from cucurbitaceae family was evaluated. In a preliminary study, effects of ethanolic extracts of Cucurbita pepo, Cucumis sativus and Praecitrullus fistulosus peels were studied at 250 and 500 mg kg−1 d−1 for 15 days in the alterations in serum glucose and in hepatic lipid peroxidation (LPO) in male mice. In the pilot experiment, the effective and safe concentration of each peel was administered (p.o.) for 10 consecutive days and then on 11th and 12th days alloxan was administered along with peel extracts. The treatment was continued up to 15th day. At the end, alterations in serum glucose, insulin, triiodothyronine, thyroxine, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, very low density lipoprotein, hepatic lipid peroxidation, superoxide dismutase and catalase were studied. All the three peel extracts nearly reversed most of these changes induced by alloxan suggesting their possible role in ameliorating diabetes mellitus and related changes in serum lipids. However, Cucurbita pepo peel was found to be the most effective. Total polyphenols, flavonoids and ascorbic acid contents of the test peels were also estimated, which appear to be associated with the observed antidiabetic and antioxidative potentials.


Alloxan Diabetes Lipid peroxidation Thyroid hormones Vegetable peels 



Lipid peroxidation—oxidative degradation of lipids


Superoxide dismutase—an endogenous antioxidant


Catalase—an endogenous antioxidant


Thiobarbituric acid—a chemical that reacts with malondialdehyde


Tricarboxylic acid—a chemical that precipitates protein


  1. 1.
    Yoshino G, Hirano T, Kazumi T (1996) Dyslipidemia in diabetes mellitus. Diab Res Clin Pract 33:1–14CrossRefGoogle Scholar
  2. 2.
    Rang HP, Dale MM (1991) The endocrine system. Pharmacology, 2nd edn. Harlow, Longman, p 504Google Scholar
  3. 3.
    Scartezzini P, Speroni E (2000) Review on some plants of the Indian traditional medicine with antioxidant activity. J Ethnopharmacol 71:23–43CrossRefGoogle Scholar
  4. 4.
    Kar A, Panda S (2004) Ayurvedic therapies for thyroid dysfunction. In: Mishra L (ed) Scientific Basis of Ayurvedic Therapies, Chapter 8. CRC Press, FLGoogle Scholar
  5. 5.
    Atawodi SE (2005) Antioxidant potential of African medicinal plants. Afr J Biotechnol 4:128–133Google Scholar
  6. 6.
    Shetty AK, Kumar GS, Sambaiah K, Salimath PV (2005) Effect of bitter gourd (Momordica charantia) on glycaemic status in streptozotocin induced diabetic rats. Plant Foods Hum Nutr 60:109–112CrossRefGoogle Scholar
  7. 7.
    Larson RA (1988) The antioxidants of higher plants. Phytochem 27:969–978CrossRefGoogle Scholar
  8. 8.
    Parmar HS, Kar A (2007) Antidiabetic potential of Citrus sinensis and Punica granatum peel extracts in alloxan treated male mice. Biofactors 31:17–24CrossRefGoogle Scholar
  9. 9.
    Parmar HS, Kar A (2007) Protective role of Citrus sinensis, Punica granatum and Musa paradisiaca peels against diet induced atherosclerosis. Nutr Res 27:710–718CrossRefGoogle Scholar
  10. 10.
    Parmar HS, Kar A (2008) Possible amelioration of atherogenic diet induced dyslipidemia, hypothyroidism and hyperglycemia by the peel extracts of Mangifera indica, Cucumis melo and Citrullus vulgaris fruits in rats. Biofactors 33:13–24CrossRefGoogle Scholar
  11. 11.
    Parmar HS, Kar A (2008) Antiperoxidative, antithyroidal, antihyperglycemic and cardioprotective role of Citrus sinensis peel extract in male mice. Phytother Res 22:791–795CrossRefGoogle Scholar
  12. 12.
    Dixit Y, Panda S, Kar A (2008) Lagenaria siceraria peel extract in the regulation of hyperthyroidism, hyperglycemia and lipid peroxidation in mice. Int J Biomed Pharma Sci 2:79–83Google Scholar
  13. 13.
    Dixit Y, Kar A (2009) Antioxidative activity of some vegetable peels determined in vitro by inducing liver lipid peroxidation. Food Res Int 42:1351–1354CrossRefGoogle Scholar
  14. 14.
    Singh N, Rajini PS (2005) Protective effect of potato peel powder in ameliorating oxidative stress in streptozotocin diabetic rats. Plant Foods Hum Nutr 60:49–54CrossRefGoogle Scholar
  15. 15.
    Park J, Kim J, Kim MK (2007) Onion flesh and onion peel enhance antioxidant status in aged rats. J Nutr Sci Vitaminol 53:21–29CrossRefGoogle Scholar
  16. 16.
    Leontowicz M, Gorinstein S, Leontowicz H, Krzeminski R, Lojek A, Katrich E, Ciz M, Martin-Belloso O, Soliva-Fortuny R, Haruenkit R, Trakhtenberg S (2003) Apple and pear peel and pulp and their influence on plasma lipids and antioxidant potentials in rats fed with cholesterol-containing diets. J Agric Food Chem 51:5780–5785CrossRefGoogle Scholar
  17. 17.
    Omaye ST, Turbull JD, Sauberlich HE (1979) Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids. Meth Enzymol 62:3–11CrossRefGoogle Scholar
  18. 18.
    Xia T, Wang Q (2006) Antihyperglycemic effect of Cucurbita ficifolia fruit extract in streptozotocin induced diabeteic rats. Fitoterapia 77:530–533CrossRefGoogle Scholar
  19. 19.
    Chandrashekhar B, Mukherjee B, Mukherjee SK (1989) Blood sugar lowering potential of selected cucurbitaceae plants of Indian origin. Indian J Med Res 90:300–305Google Scholar
  20. 20.
    Raskovic A, Gavrilovic M, Jakovljevic V, Sabo J (2004) Glucose concentration in the blood of intact and alloxan-treated mice after pretreatment with commercial preparations of Stevia rebaudiana (Bertoni). Eur J Drug Metab Pharmacokinet 29:87–90CrossRefGoogle Scholar
  21. 21.
    Panda S, Kar A (2007) Antidiabetic and antioxidative effects of Annona squamosa leaves are possibly mediated through quercetin-3-O-glucoside. Biofactors 31:201–210CrossRefGoogle Scholar
  22. 22.
    Jatwa R, Parmar HS, Panda S, Kar A (2007) Amelioration of corticosteroid-induced type 2 diabetes mellitus by rosiglitazone is possibly mediated through stimulation of thyroid function and inhibition of tissue lipid peroxidation in mice. Basic Clin Pharmacol Toxicol 101:177–180CrossRefGoogle Scholar
  23. 23.
    Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 95:351–358CrossRefGoogle Scholar
  24. 24.
    Marklund S, Marklund G (1974) Involvement of superoxide anion radical in antioxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur J Biochem 47:469–474CrossRefGoogle Scholar
  25. 25.
    Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods in Enzymatic Analysis (Vol III). Academic Press, New York, pp 276–286Google Scholar
  26. 26.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275Google Scholar
  27. 27.
    Trinder P (1969) Practical Clinical Biochemistry (Vol X), 5th edn. William Heinamman Medical books LTD, New YorkGoogle Scholar
  28. 28.
    Parmar HS, Kar A (2007) Atherogenic diet induced diabetes mellitus: involvement of thyroid hormones. Eur J Pharmacol 570:244–248CrossRefGoogle Scholar
  29. 29.
    Parmar HS, Kar A (2009) Protective role of Mangifera indica, Cucumis melo and Citrullus vulgaris peel extracts in chemically induced hypothyroidism. Chem Biol Interact 177:254–258CrossRefGoogle Scholar
  30. 30.
    Rerup CC (1970) Drugs producing diabetes through damage of insulin secreting cells. Pharmacol Rev 22:485–520Google Scholar
  31. 31.
    Yallow RS, Black H, Villazan M, Berson SA (1960) Comparison of plasma insulin levels following administration of tolbutamide and glucose. Diabetes 9:356–362Google Scholar
  32. 32.
    Grodsky GM, Epstein GH, Fanska R, Karam JH (1971) Pancreatic action of sulphonylureas. Fed Proc 36:2719–2728Google Scholar
  33. 33.
    Gray M, Flatt PR (1999) Insulin-secreting activity of the traditional antidiabetic plant Viscum album (mistletoe). J Endocrinol 160:409–414CrossRefGoogle Scholar
  34. 34.
    Parmar HS, Kar A (2008) Medicinal values of fruit peels from Citrus sinensis, Punica granatum, and Musa paradisiaca with respect to alterations in tissue lipid peroxidation and serum concentration of glucose, insulin and thyroid hormones. J Med Food 11:376–381CrossRefGoogle Scholar
  35. 35.
    Parmar HS, Panda S, Jatwa R, Kar A (2006) Cardio-protective role of Terminalia arjuna bark extract is possibly mediated through alterations in thyroid hormones. Pharmazie 61:793–795Google Scholar
  36. 36.
    Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50:537–546Google Scholar
  37. 37.
    Iqbal MP, Kazim SF, Mehboobali N (2006) Ascorbic acid contents of Pakistani fruits and vegetables. Pak J Pharm Sci 19:282–285Google Scholar
  38. 38.
    Marinova D, Ribarova F, Atanassova M (2005) Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metallurgy 40:255–260Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Endocrine Research Unit, School of Life Sciences,Takshashila CampusDevi Ahilya VishwavidhyalayaIndoreIndia

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