Plant Foods for Human Nutrition

, Volume 65, Issue 1, pp 18–24 | Cite as

Inhibition of Iron Induced Lipid Peroxidation and Antioxidant Activity of Indian Spices and Acacia In Vitro

  • Amit Singh Yadav
  • Deepak BhatnagarEmail author
Original Paper


The spices used in the Indian foods such as Star anise (Illicium verum), Bay leaves (Cinnamomum zeylanicum) and Cobra’s saffron (Mesua ferrea), and Acacia (Acacia catechu), which have medicinal value, were used as test samples, to find their effect on in vitro lipid peroxidation (LPO). Rat liver post mitochondrial supernatant (PMS) in Tris HCl buffer, pH 7.4 was incubated for 0 and 1 h, with various test extracts in three different oxidant systems. The results show that addition of test samples to FeCl3 medium at 0 h significantly stop the initiation of the LPO. However, the propagation phase of LPO was inhibited by Cobra’s saffron and Acacia and not by Star anise and Bay leaves. The test samples also showed strong reducing power and superoxide radical scavenging activity. Cobra’s saffron and Acacia showed the highest antioxidant activity, probably due to the higher polyphenol content as compared to other test samples.


Reactive oxygen species Lipid peroxidation Antioxidant Reducing power 



We thank Dr. Shakti Banerjee, Professor, School of Statistics, Devi Ahilya University, Indore, for his help in statistical analysis.


  1. 1.
    Sies H (1991) Oxidative stress, oxidants and antioxidants. Academic, San DiegoGoogle Scholar
  2. 2.
    Halliwell B, Gutteridge JMC, Cross CE (1992) Free radicals, antioxidants and human disease: where we are now. J Lab Clin Med 119:598–620Google Scholar
  3. 3.
    Formica JV, Regelson W (1995) Review of the biology of quercetin and related bioflavonoids. Food Chem Toxicol 33:1061–1080CrossRefGoogle Scholar
  4. 4.
    Yang CS, Landau JM, Huang MT, Newmark HL (2001) Inhibition of carcinogenesis by dietary polyphenolic compounds. Ann Rev Nutr 2:381–406CrossRefGoogle Scholar
  5. 5.
    Prior RL (2003) Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clin Nutr 78(Supp):570S–578SGoogle Scholar
  6. 6.
    Spiteller G (1996) Enzymic lipid peroxidation—a consequence of cell injury. Free Rad Biol Med 21:1003–1009CrossRefGoogle Scholar
  7. 7.
    Sreejayan N, Rao MNA (1993) Curcumin inhibits iron dependent lipid peroxidation. Int J Pharma 100:93–97CrossRefGoogle Scholar
  8. 8.
    Esterbauer H, Cheeseman HK, Dianzani MU, Poli G, Slater TF (1982) Seperation and characterization of the aldehydic products of LPO stimulated by ADP-Fe2+ in rat liver microsomes. Biochem J 208:129–140Google Scholar
  9. 9.
    Oyaizu M (1986) Studies on product of browning reaction prepared from glucose amine. Japan J Nutr 44:307–315Google Scholar
  10. 10.
    Liu F, Ooi VEC, Chang ST (1997) Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci 60:763–771CrossRefGoogle Scholar
  11. 11.
    Lotito SB, Frei B (2004) Relevance of apple polyphenols as oxidants in human plasma: contrasting in vitro and in vivo effects. Free Rad Biol Med 36:201–211Google Scholar
  12. 12.
    Gulcin I, Mshvildadze V, Gepdiremen A, Elias R (2006) Screening of antioxidant and antiradical activity of monodesmosides and crude extract from Leontice smirnowii tuber. Phytomed 13:343–351CrossRefGoogle Scholar
  13. 13.
    Gulcin I (2009) Antioxidant activity of L- adrenaline: an activity-structure insight. Chem Biol Interact 179:71–80CrossRefGoogle Scholar
  14. 14.
    Kell DB (2009) Iron behaving badly: inappropriate iron chelation as a major contributor to the aetiology of vascular and other progressive inflammatory and degenerative diseases. BMC Med Genomics 2:2. doi: 10.1186/1755-8794 CrossRefGoogle Scholar
  15. 15.
    Prakash D, Suri S, Upadhyay G, Singh BN (2007) Total phenol, antioxidant and free radical scavenging activities of some medicinal plants. Int J Food Sci Nutr 58:18–28CrossRefGoogle Scholar
  16. 16.
    Jang IC, Park JH, Park E, Park HR, Lee SC (2008) Antioxidative and antigenotoxic activity of extracts from Cosmos (Cosmos bipinnatus) flowers. Plant Foods Hum Nutr 63:205–210CrossRefGoogle Scholar
  17. 17.
    Bystrom LM, Lewis BA, Brown DL, Rodriguez E, Obendorf RL (2009) Phenolics, sugars, antimicrobial and free-radical-scavenging activities of Melicoccus bijugatus Jacq. Fruits from the Dominican Republic and Florida. Plant Foods Hum Nutr 64:160–166CrossRefGoogle Scholar
  18. 18.
    Tung YT, Wu JH, Kuo YH, Chang ST (2007) Antioxidant activities of natural phenolic compounds from Acacia confusa bark. Biores Tech 98:1120–1123CrossRefGoogle Scholar
  19. 19.
    Yadav AS, Bhatnagar D (2007) Modulatory effect of spice extracts on iron induced lipid peroxidation in rat liver. BioFactors 29:147–157CrossRefGoogle Scholar
  20. 20.
    Yadav AS, Bhatnagar D (2007) Free radical scavenging activity, metal chelation and antioxidant power of some of the Indian spices. BioFactors 31:219–228CrossRefGoogle Scholar
  21. 21.
    Yadav AS, Bhatnagar D (2007) Chemopreventive effect of Star anise in N- nitrosodiethylamine initiated and phenobarbital promoted hepatocarcinogenesis. Chem Biol Interact 169:207–214CrossRefGoogle Scholar
  22. 22.
    Moridani MY, Pourahmad J, Bui H, Siraki A, O’Brien PJ (2003) Dietary flavonoids iron complexes as cytoprotective superoxide radical scavengers. Free Rad Biol Med 34:243–253CrossRefGoogle Scholar
  23. 23.
    Afanas’ev IB, Ostrachovich EA, Korkina LG (1998) Effect of rutin and its copper complex in superoxide formation and lipid peroxidation in rat liver microsomes. FEBS Lett 425:256–258CrossRefGoogle Scholar
  24. 24.
    Van Acker SA, van Balen GP, van den Bergh DJ, Bast A, van der Vijgh WJ (1998) Influence of iron chelation on the antioxidant activity of flavonoids. Biochem Pharmacol 62:743–746Google Scholar
  25. 25.
    Sroka Z, Cisowski W (2003) Hydrogen peroxide scavenging, antioxidant and antiradical activity of some phenolic acids. Food Chem Toxicol 41:753–758CrossRefGoogle Scholar
  26. 26.
    Dudonne S, Vitrac X, Coutiere P, Woillez M, Merillon JM (2009) Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem 57:1768–1774CrossRefGoogle Scholar
  27. 27.
    Katalinic V, Milos M, Kulisic T, Jukic M (2006) Screening of 70 medicinal plants extracts for antioxidant capacity and total phenols. Food Chem 94:550–557CrossRefGoogle Scholar
  28. 28.
    Wong CC, Li HB, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97:705–711CrossRefGoogle Scholar
  29. 29.
    Rice-Evans CA, Miller NJ, Paganga G (1996) Structure-antioxidant activity relationships of flavonoids and phenolic acid. Free Rad Biol Med 20:933–956CrossRefGoogle Scholar
  30. 30.
    Bors W, Michel C (2002) Chemistry of the antioxidant effect of polyphenols. Ann NY Acad Sci 957:57–69CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.School of BiochemistryDevi Ahilya UniversityIndoreIndia

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