Plant Foods for Human Nutrition

, Volume 64, Issue 2, pp 116–121 | Cite as

Antihyperglycemic, Antioxidant and Antiglycation Activities of Mulberry Leaf Extract in Streptozotocin-Induced Chronic Diabetic Rats

  • Jarinyaporn Naowaboot
  • Patchareewan Pannangpetch
  • Veerapol Kukongviriyapan
  • Bunkerd Kongyingyoes
  • Upa kukongviriyapan
Original Paper


In Thailand, beverages containing mulberry leaf (Morus alba L.) are believed to promote good health, especially in people with diabetes. We examined the effects of long-term administration of an ethanolic extract of mulberry leaf (MA) on blood glucose, oxidative damage, and glycation in streptozotocin-induced diabetic rats. Daily administration of 1 g/kg MA for six weeks decreased blood glucose by 22%, which was comparable to the effect of 4 U/kg insulin. Lipid peroxidation, measured as malondialdehyde and lipid hydroperoxide concentrations (3.50 ± 0.33 and 3.76 ± 0.18 μM, respectively) decreased significantly (P < 0.05) compared to nontreated control diabetic rats (8.19 ± 0.45 and 7.50 ± 0.46 μM, respectively). Hemoglobin A1C, a biomarker for chronic exposure to high concentration of glucose, was also significantly decreased in the MA-treated group (6.78 ± 0.30%) in comparison to untreated group (9.02 ± 0.30%). The IC50 of in vitro antiglycation and free radical scavenging activities of MA were 16.4 ± 5.6 μg/ml and 61.7 ± 2.1 μg/ml, respectively. These findings support that long-term administration of MA has antihyperglycemic, antioxidant and antiglycation effects in chronic diabetic rats, which may be beneficial as food supplement for diabetics.


Antiglycation Antioxidant Diabetes mellitus Morus alba Mulberry leaf 



Advanced glycation endproducts


Morus alba-ethanolic extract







This work was supported by the Faculty of Medicine and Graduate School, Khon Kaen University (Grant number i50111). Miss Jarinyaporn Naowaboot is a graduate student supported by the Royal Golden Jubilee Ph.D. Program Grant, Thailand Research Fund.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jarinyaporn Naowaboot
    • 1
  • Patchareewan Pannangpetch
    • 1
  • Veerapol Kukongviriyapan
    • 1
  • Bunkerd Kongyingyoes
    • 1
  • Upa kukongviriyapan
    • 2
  1. 1.Department of Pharmacology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand
  2. 2.Department of Physiology, Faculty of MedicineKhon Kaen UniversityKhon KaenThailand

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