Hepatic Protection by Noni Fruit Juice Against CCl4-Induced Chronic Liver Damage in Female SD Rats
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Morinda citrifolia L. (noni) has been used throughout the Pacific, Southeast Asia, Central America, and the Caribbean for a variety of health conditions, including heart and liver ailments. In this study, we examined the hepatoprotective effects of TAHITIAN NONI® Juice (TNJ) against CCl4-induced chronic liver damage in female Sprague Dawley (SD) rats. Twelve female SD rats were divided into control, placebo and TNJ (6 mL/rat/day) groups. On day 15, animals in the placebo and TNJ groups received 0.25 mL/kg CCl4 in corn oil once a week for 12 successive weeks. All animals were sacrificed at week 16. Blood and liver were collected for liver function, lipid panel tests, and histological observation. Histopathological examination revealed that liver sections from the TNJ + CCl4 appeared similar to controls, whereas typical hepatic steatosis was observed in the placebo + CCl4 group. Serum alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and very low-density lipoprotein (VLDL) levels were increased in the placebo group compared with the TNJ group. In contrast, high-density lipoprotein (HDL) was increased in the TNJ group and decreased in the placebo group. Thus, TNJ juice appears to protect the liver from chronic exogenous CCl4 exposures. Such protective mechanisms are supportive evidence for the utility of noni in traditional medicine for liver ailments.
KeywordsMorinda citrifolia L. (Rubiaceae) (noni) Carbon tetrachloride Hepatic protection
This study was financially supported by Research grant of Morinda Holdings Inc. We thank Dr. Richard Novak and Dr. Leland White for editing the article and manuscript preparation. We thank Brett J. West for formulation and production of the placebo. We thank the clinical technicians of the laboratory of Family Health Center of UIC College of Medicine for their technical help in the liver function and lipid panel tests. A special thanks is extended to Mr. John Javaherian for his efforts on animal care.
- 1.Wang MY, West BJ, Jensen CJ, Nowicki D, Chen S, Palu A, Anderson G (2002) Morinda citrifolia (Noni) a literature review and recent advances in noni research. Acta Pharmacol Sin 23(12):1127–1141Google Scholar
- 2.Glassman SF (1952) The flora of Ponape. Honolulu (Hi): B.P. Bishop Museum, Bulletin 209; 152 ppGoogle Scholar
- 3.Burkill IH (1966) A dictionary of the economic products of the Malay Peninsula. vol II. Kuala Lumpur (Malaysia): Ministry of Agriculture and Co-operatives; p 1515–1519Google Scholar
- 4.Morton JF (1981) Atlas of medicinal plants of Middle America: Bahamas to Yucatan. Springfield (Ill): C.C. Thomas. Figure 463, Morinda citrifolia; p 868Google Scholar
- 16.Habib SS (2004) Lipoprotein(a): the bad cholesterol. Saudi Med J 25:429–433Google Scholar
- 17.Hodoglugil U, Mahley RW (2006) Smoking and obesity make a bad problem worse: genetics and lifestyle affect high density lipoprotein levels in Turks. Anadolu Kardiyol Dergisi 6:60–67Google Scholar
- 18.Baratta PF, Angeli G, Lusiani GB, Cavazzuti F (1954) Effect of heparin on hepatic steatosis due to hypercholesterol diet. Bollettino-Societa Italiana Biologia Sperimentale (Napoli) 30:164–166Google Scholar
- 29.Marsh JB (1976) Apoproteins of the lipoproteins in a nonrecirculating perfusate of rat liver. J Lipid Res 17:85–89Google Scholar
- 31.Chan DC, Barrett PH, Watts GF (2004) Lipoprotein transport in the metabolic syndrome: pathophysiological and interventional studies employing stable isotopy and modelling methods. Clin Sci (London, England) 107:233–249Google Scholar
- 32.Wang M-Y, Su C, Nowicki D, Jensen J, Anderson G. Protective effect of Morinda citrifolia in carbon tetrachloride-induced liver injury model: A light and electron microscopic study. In: Proceedings of the Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Related Diseases; 2001 October 14–17; 7th Annual Conference; Nashville, Tennessee, USA. (P15)Google Scholar