Plant Foods for Human Nutrition

, Volume 63, Issue 2, pp 65–70 | Cite as

Extensive Screening for Edible Herbal Extracts with Potent Scavenging Activity against Superoxide Anions

  • Keita Saito
  • Masahiro Kohno
  • Fumihiko Yoshizaki
  • Yoshimi NiwanoEmail author
Original Paper


To search for edible herbal extracts with potent antioxidant activity, we conducted a large scale screening based on the superoxide scavenging activity. That is, scavenging activity against superoxide anions were extensively screened from ethanol extracts of approximately 1,000 kinds of herbs by applying an electron spin resonance (ESR)-spin trapping method. Among them we chose four edible herbal extracts with prominently potent ability to reduce the signal intensity of 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)–OOH, a spin adduct formed by DMPO and superoxide anion. They are the extracts from Punica granatum (Peel), Syzygium aromaticum (Bud), Mangifera indica (Kernel), and Phyllanthus emblica (Fruit), and are allowed to be used as foodstuffs according to the Japanese legal regulation. The ESR-spin trapping method coupled with steady state kinetic analysis showed that all of the four extracts directly scavenge superoxide anions, and that the superoxide scavenging potential of any of the extracts was comparable to that of L-ascorbic acid. Furthermore, polyphenol determination indicates that the activity is at least in part attributable to polyphenols. These results with such large scale screening might give useful information when choosing a potent antioxidant as a foodstuff.


Edible herbal extracts Superoxide scavenging activity 



superoxide dismutase


electron spin resonance




xanthine oxidase





This work was supported by a grant of Kampou Science Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Keita Saito
    • 1
  • Masahiro Kohno
    • 1
  • Fumihiko Yoshizaki
    • 3
  • Yoshimi Niwano
    • 1
    • 2
    Email author
  1. 1.New Industry Creation Hatchery CenterTohoku UniversitySendaiJapan
  2. 2.Research Center for Functional Food Materials, Sunny Health Holdings Co., Ltd.Saito Biotechnology Incubator7-7-15 Saito-Asagi, Ibaraki, OsakaJapan
  3. 3.Department of PharmacognosyTohoku Pharmaceutical UniversitySendaiJapan

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