Plant Foods for Human Nutrition

, Volume 61, Issue 3, pp 145–150 | Cite as

Effect of Cultivar, Year Grown, and Cropping System on the Content of Tocopherols and Tocotrienols in Grains of Hulled and Hulless Barley

  • J. Ehrenbergerová
  • N. Belcrediová
  • J. Prýma
  • K. Vaculová
  • C. W. Newman


In a three-year period (2000–2002) total tocols (tocopherols and tocotrienols), content of vitamin E and its isomers (α-, β+γ-, δ-tocopherols and tocotrienols) were assessed in grain of 13 barley genotypes. The highest content of tocols (60.3–67.6 mg kg−1) and content of vitamin E (Vitamin E equivalent—18.0–20.1 mg kg−1) were determined in the waxy varieties Wanubet, Wabet, and Washonubet. Standard varieties, i.e. of a malting type (Krona and Kompakt), had statistically significantly lower content of tocols (49.9 and 53.6 mg kg−1) and vitamin E (15.7–16.1 mg kg−1) compared to the waxy varieties. The hulless waxy variety Washonubet had statistically significantly higher total content of tocols (67.6 mg kg−1) and α- tocotrienols isomer (42.1 mg kg−1) versus all other genotypes in the set. Chemical treatment and fertilization statistically significantly increased the content of tocols (by 4.7 mg kg−1), vitamin E (by 1.9 mg kg−1), isomer α-tocopherol (by 0.9 mg kg−1) and isomer α- tocotrienols (by 3.3 mg kg−1). The average values of α-tocopherols and α-tocotrienols in the set were 6.7 mg kg−1 and 29.7 mg kg−1, respectively. Some of the reciprocal lines created by us from the malting and waxy varieties are suitable for food use for high contents of all tocopherols and α-tocotrienols.

Key words:

Spring barley Waxy barley Tocopherols Tocotrienols Cropping system 


Ko =


Kr =


T =


T3 =


VEeq =

Vitamin E equivalent

Wb =


Wnb =


Wsnb =



weight of a thousand grains



The authors acknowledge financial support by the Ministry of Educations, Youth and Sports of the Czech Republic, Project RC No. 1M0570 and by the Grant Agency of the Czech Republic, Project No. 525/05/0781.


  1. 1.
    Kerckhoffs DA, Brouns F, Hornstra G, Mensink RP (2002) Effect on the human lipoprotein profile of β-glucan, soy protein and isoflavones, plant sterols and stanols, garlic and tocotrienols. J Nutr 132: 2494–2505.Google Scholar
  2. 2.
    Hunt SM, Groff JL (1990) Advanced Nutrition and Human Metabolism. St. Paul, MN: West Publishing Company.Google Scholar
  3. 3.
    Cahoon EB, Hall SE, Ripp KG, Ganzke TS, Hits WD, Coughlan SJ (2003) Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content. Nature Biotech 21: 1082–1087.CrossRefGoogle Scholar
  4. 4.
    Qureshi AA, Qureshi N, Hasler-Rapacz JO, Weber FE, Chaudhary V, Crenshaw TD, Gapro A, Ong ASH, Chong YH (1991) Dietary tocotrienols reduce concentrations of plasma cholesterol, apoliopoprotein B, thromboxane B, and platelet factor 4 in pigs with inherited hyperlipidemias. Am J Clin Nutr 53: 1042S–10426S.Google Scholar
  5. 5.
    Qureshi AA, Bradlow BA, Brace L, Manganello J, Peterson DM, Pearce BC, Wright JJ, Gapor A, Elson CE (1995) Response of hypercholesterolemic subjects to the administration of tocotrienol. Lipids 30: 1117–1119.Google Scholar
  6. 6.
    Watkins T, Lenz P, Gapor A, Struck M, Tomeo A, Bierenbarum M (1993) γ-tocotrienol as a hypocholesterolemic and antioxidant agent in rats fed atherogenic diets. Lipids 28: 1113–1118.Google Scholar
  7. 7.
    Qureshi AA, Burger WC, Peterson DM, Elson CE (1986) The structure of an inhibitor of cholesterol biosynthesis isolated from barley. J Biol Chem 261: 10544–10550.Google Scholar
  8. 8.
    Qureshi AA, Burger WC, Peterson DM, Elson CE (1985) Suppression of cholesterogenesis by plant constituents: Review of Wisconsin contributions to NC-167. Lipids 20: 817–824.Google Scholar
  9. 9.
    Wang LJ, Newman RK, Newman CW, Jackson LL, Hofer PJ (1993) Tocotrienol and fatty acid composition of barley oil and effects on lipid metabolism. Plant Foods for Hum Nutr 43: 9–17.CrossRefGoogle Scholar
  10. 10.
    Weber FE, Chaudhary VK, Lupton JR, Qureshi AA (1990) Therapeutic and physiological properties of barley bran. Cereal Foods World 35: 844–846.Google Scholar
  11. 11.
    Theriault A, Chao JT, Wang Q, Gapor A, Adeli K (1999) Tocotrienol: A review of its therapeutic potential. Clinical Biochem 32: 309–319.CrossRefGoogle Scholar
  12. 12.
    EN 12 822:2000, Foodstuffs – Determination of vitamin E by high performance liquid chromatography–Measurement of α-, β-, γ-, and δ- tocopherols, European committee for standardization, B-1050 Brussels, February 2000.Google Scholar
  13. 13.
    McLaughlin PJ, Weihrauch JL (1979) Vitamin E content of foods. J Am Dietet Assoc 75: 647–651.Google Scholar
  14. 14.
    Peterson DM, Qureshi AA (1993) Genotype and environment effects of tocols of barley and oats. Cereal Chem 70: 157–162.CrossRefGoogle Scholar
  15. 15.
    Bhatty RS (1999) The potential of hulless barley. Cereal Chem 76: 589–599.Google Scholar
  16. 16.
    Peterson DN (1994) Barley tocols: Effects of milling, malting and mashing. Cereal Chem 71: 42–44.Google Scholar
  17. 17.
    Cavalero A, Gianinetti A, Finocchiaro F, Delogu G, Stanca AM (2004) Tocols in hull-less and hulled barley genotypes grown in contrasting environments. J Cereal Sci 39: 175–180.CrossRefGoogle Scholar
  18. 18.
    Vaculova K, Ehrenbergerova J, Nemejc R.V, Pryma J (2001) The variability and correlations between the content of vitamin E and its isomers in hybrids of the F2 generation of spring barley. Acta Univ. agric. et silvic. Mendel. Brun. XLIX 1, 1–9.Google Scholar
  19. 19.
    Goupy P, Hugues M, Boivin JP, Amiot MJ (1999) Antioxidant Compounds and Activity of Barley (Hordeum vulgare) and Malt Extracts. Cannes: EBC Congress, pp 445–451.Google Scholar
  20. 20.
    Holasova M, Velisek J, Davidek J (1995) Tocopherol and tocotrienol contents in cereal grains. Potravinarske Vedy 13: 409–417.Google Scholar
  21. 21.
    Cavallero A, Viva M, Stanca AM (2000) Improvement of spaghetti and bread with β-glucan and tocols from naked barley. In: Barley Genetics VIII, Proceedings of the VIII International Barley Genetics Symposium. Adelaide Australia, pp 282–1087.Google Scholar
  22. 22.
    Holasova M, Velisek J, Davidek J (1998) Cereal grains-the source of tocopherols and tocotrienols. In: Proceedings, International Conference of Cereal for Human Health and Preventive Nutrition, Brno Czech Rep, pp 185–187.Google Scholar
  23. 23.
    Colombo ML, Corsini A, Cattivelli L, Arlandi E, Remo F, Paoletti R., Pank F (1996) Breeding research on medicinal plants. In: Proceedings of the International Symposium Beiträge zur Züchtungsforschung Bundesanstalt fur Züchtungsforschung und Kulturpflazen. Quedlinburg Germany pp. 393–395.Google Scholar

Copyright information

© Springer Science+Business Media, Inc 2006

Authors and Affiliations

  • J. Ehrenbergerová
    • 1
  • N. Belcrediová
    • 1
  • J. Prýma
    • 2
  • K. Vaculová
    • 3
  • C. W. Newman
    • 4
  1. 1.Mendel University of Agriculture and ForestryBrnoCzech Republic
  2. 2.Research Institute of Brewing and MaltingPLC, Malting InstituteBrnoCzech Republic
  3. 3.Agricultural Research InstituteKroměříž, Ltd.KroměřížCzech Republic
  4. 4.Montana State UniversityBozemanUSA

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