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Stark fluorescence spectroscopy on peridinin–chlorophyll–protein complex of dinoflagellate, Amphidinium carterae

  • Anjue Mane Ara
  • Md. Shakil Bin Kashem
  • Rienk van Grondelle
  • Md. WahadoszamenEmail author
Original article
  • 67 Downloads

Abstract

Because of their peculiar but intriguing photophysical properties, peridinin–chlorophyll–protein complexes (PCPs), the peripheral light-harvesting antenna complexes of photosynthetic dinoflagellates have been unique targets of multidimensional theoretical and experimental investigations over the last few decades. The major light-harvesting chlorophyll a (Chl a) pigments of PCP are hypothesized to be spectroscopically heterogeneous. To study the spectral heterogeneity in terms of electrostatic parameters, we, in this study, implemented Stark fluorescence spectroscopy on PCP isolated from the dinoflagellate Amphidinium carterae. The comprehensive theoretical modeling of the Stark fluorescence spectrum with the help of the conventional Liptay formalism revealed the simultaneous presence of three emission bands in the fluorescence spectrum of PCP recorded upon excitation of peridinin. The three emission bands are found to possess different sets of electrostatic parameters with essentially increasing magnitude of charge-transfer character from the blue to redder ones. The different magnitudes of electrostatic parameters give good support to the earlier proposition that the spectral heterogeneity in PCP results from emissive Chl a clusters anchored at a different sites and domains within the protein network.

Keywords

Light harvesting Energy transfer Peridinin–chlorophyll–protein complexes Charge-transfer states Stark spectroscopy Spectral heterogeneity 

Notes

Acknowledgements

Md. W., A. M. A., and R. v. G. were supported by the VU University Amsterdam, the Laserlab-Europe Consortium and the advanced investigator Grant (267333, PHOTPROT) from the European Research Council. Md. W. and R. v. G. were supported further by the TOP grant (700.58.305) from the Foundation of Chemical Sciences part of NWO.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11120_2019_688_MOESM1_ESM.docx (196 kb)
Supplementary material 1 (DOCX 196 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Biophysics of Photosynthesis, Department of Physics and Astronomy, Faculty of SciencesVU University AmsterdamAmsterdamThe Netherlands
  2. 2.Department of PhysicsJagannath UniversityDhakaBangladesh
  3. 3.Department of PhysicsUniversity of DhakaDhakaBangladesh

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