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The in vitro synergistic denaturation effect of heat and surfactant on photosystem I isolated from Arthrospira Platensis

  • Daoyong YuEmail author
  • Jinxiao Lan
  • Naseer Ullah Khan
  • Quan Li
  • Fengxi Xu
  • Guihong Huang
  • Hai XuEmail author
  • Fang HuangEmail author
Original Article
  • 24 Downloads

Abstract

Photosystem I (PSI) generates the most negative redox potential found in nature, and the performance of solar energy conversion into alternative energy sources in artificial systems highly depends on the thermal stability of PSI. Thus, understanding thermal denaturation is an important prerequisite for the use of PSI at elevated temperatures. To assess the thermal stability of surfactant-solubilized PSI from cyanobacteria Arthrospira Platensis, the synergistic denaturation effect of heat and surfactant was studied. At room temperature, surfactant n-dodecyl-β-d-maltoside solubilized PSI trimer gradually disassembles into PSI monomers and free pigments over long time. In the solubilizing process of PSI particles, surfactant can uncouple pigments of PSI, and the high concentration of surfactant causes the pigment to uncouple more; after the surfactant-solubilizing process, the uncoupling is relatively slow. During the heating process, changes were monitored by transmittance T800nm, ellipticity θ686nm and θ222nm, upon slow heating (1.5 °C per minute) of samples in Tris buffer (20 mM, pH 7.8) from 20 to 95 °C. The thermal denaturation of surfactant-solubilized PSI is a much more complicated process, which includes the uncoupling of pigments by surfactants, the disappearance of surrounding surfactants, and the unfolding of PSI α-helices. During the heating process, the uncoupling chlorophyll a (Chla) and converted pheophytin (Pheo) can form excitons of Chla–Pheo. The secondary structure α-helix of PSI proteins is stable up to 87–92 °C in the low-concentration surfactant solubilized PSI, and high-concentration surfactant and pigments uncoupling can accelerate the α-helical unfolding.

Keywords

Photosystem I Surfactant Solubilization Structural integrity Thermal stability 

Abbreviations

PSI

Photosystem I

DDM

n-Dodecyl-β-d-maltoside

TX

Triton X-100

CMC

Critical micelle concentration

CD

Circular dichroism

DLS

Dynamic light scattering

Chl

Chlorophyll

Chla

Chlorophyll a

Phe

Pheophytin

T800nm

Transmittance at 800 nm

θ686nm

Ellipticity at 686 nm

θ222nm

Ellipticity at 222 nm

A. platensis

Arthrospira platensis (old name: Spirulina platensis)

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21673292), Shandong Provincial Natural Science Foundation (ZR2014BM013), and the Fundamental Research Funds for the Central Universities. The content is solely the responsibility of the authors and does not necessarily represent the official views of the supporters.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11120_2019_623_MOESM1_ESM.pdf (243 kb)
Supplementary material 1 (PDF 242 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Heavy Oil ProcessingChina University of Petroleum (East China)QingdaoChina
  2. 2.Center for Bioengineering and Biotechnology, College of Chemical EngineeringChina University of Petroleum (East China)QingdaoChina

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