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Photosynthesis Research

, Volume 139, Issue 1–3, pp 461–473 | Cite as

Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light–dark cycle

  • Cosmin Ionel SicoraEmail author
  • Iuliana Chiș
  • Ciprian Chiș
  • Oana Sicora
Original Article
  • 91 Downloads

Abstract

Cyanobacteria, as well as green algae and higher plants, have highly conserved photosynthetic machinery. Cyanothece sp. ATCC 51142 is a unicellular, aerobic, diazotrophic cyanobacterium that fixes N2 in the dark. In Cyanothece, the psbA gene family is composed of five members, encoding different isoforms of the D1 protein. A new D1 protein has been postulated in the literature, which blocks PSII during the night and allows the fixation of nitrogen. We present data showing changes in PSII function in cells grown in cycles alternating between 12 h of light and dark, respectively, at Cyanothece sp. ATCC 51142. Cyanothece sp. ATCC 51142 uses intrinsic mechanisms to protect its nitrogenase activity in a two-stage process. In Stage I, immediately after the onset of darkness, the cells lose photosynthetic activity in a reversible process, probably by dissociation of water oxidation complex from photosystem II via a mechanism that does not require de novo protein synthesis. In Stage II, a more severe disruption of photosystem II function occurs is in part protein synthesis dependent and it could be a functional signature of the presence of sentinel D1 in a limited number of reaction centers still active or not yet inactivated by the mechanism described in Stage I. This process of inhibition uses light as a triggering signal for both the inhibition of photosynthetic activity and recovery when light returns. The intrinsic mechanism of photosynthetic inactivation during darkness with the interplay of the two mechanisms requires further studies.

Keywords

Cyanothece Photosystem II D1 sentinel (rogue) Light/dark cycle Flash fluorescence 

Notes

Acknowledgements

The project was partially funded by the Romanian National Authority for Scientific Research, CNCS-UEFISCDI, project number: PN-II-IDPCE-2011-3-0765 and the Executive Unit for Financing Higher Education, Research, Development and Innovation (UEFISCDI), Romania, project code PN-II-RU-PD-2011-3-0255.

Supplementary material

11120_2018_598_MOESM1_ESM.pdf (421 kb)
Supplementary material 1 (PDF 421 KB)
11120_2018_598_MOESM2_ESM.pdf (434 kb)
Supplementary material 2 (PDF 433 KB)
11120_2018_598_MOESM3_ESM.pdf (373 kb)
Supplementary material 3 (PDF 372 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Cosmin Ionel Sicora
    • 1
    Email author
  • Iuliana Chiș
    • 1
  • Ciprian Chiș
    • 1
  • Oana Sicora
    • 1
  1. 1.Biological Research Center JibouJibouRomania

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