Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile
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The soil chromophyte alga Xanthonema (X.) debile contains only non-carbonyl carotenoids and Chl-a. X. debile has an antenna system denoted Xanthophyte light-harvesting complex (XLH) that contains the carotenoids diadinoxanthin, heteroxanthin, and vaucheriaxanthin. The XLH pigment stoichiometry was calculated by chromatographic techniques and the pigment-binding structure studied by resonance Raman spectroscopy. The pigment ratio obtained by HPLC was found to be close to 8:1:2:1 Chl-a:heteroxanthin:diadinoxanthin:vaucheriaxanthin. The resonance Raman spectra suggest the presence of 8–10 Chl-a, all of which are 5-coordinated to the central Mg, with 1–3 Chl-a possessing a macrocycle distorted from the relaxed conformation. The three populations of carotenoids are in the all-trans configuration. Vaucheriaxanthin absorbs around 500–530 nm, diadinoxanthin at 494 nm and heteroxanthin at 487 nm at 4.5 K. The effective conjugation length of heteroxanthin and diadinoxanthin has been determined as 9.4 in both cases; the environment polarizability of the heteroxanthin and diadinoxanthin binding pockets is 0.270 and 0.305, respectively.
KeywordsLight-harvesting complex Algae Resonance Raman Chl-a Carotenoids Diadinoxanthin Heteroxanthin
This work was supported by the ERC funding agency (PHOTPROT project), the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05, and the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant agreement No 675006 (SE2B). The research in the Czech Republic was supported by the Czech Science Foundation Grant P501/12/G055, European Regional Development Fund (No. CZ.02.1.01/0.0/0.0/15_003/0000441), and by institutional funding RVO:60077344.
- Andreoli C, Moro I, La Rocca N, Rigoni F, Valle LD, Bargelloni L (1999) Pseudopleurochloris antarctica gen. et sp. nov., a new coccoid Xanthophycean from pack-ice of Wood Bay (Ross Sea, Antarctica): ultrastructure, pigments and 18S rRNA gene sequence. Eur J Phycol 34(2):149–159. https://doi.org/10.1080/09670269910001736202 CrossRefGoogle Scholar
- Frank HA, Cua A, Chynwat V, Young A, Gosztola D, Wasielewski MR (1996) The lifetimes and energies of the first excited singlet states of diadinoxanthin and diatoxanthin: the role of these molecules in excess energy dissipation in algae. Biochim Biophys Acta Bioenergy 1277(3):243–252. https://doi.org/10.1016/S0005-2728(96)00106-5 CrossRefGoogle Scholar
- Jeffrey SW, Mantoura RFC, Wright SW, International Council of Scientific Unions. Scientific Committee on Oceanic Research (1997) Phytoplankton pigments in oceanography: guidelines to modern methods. UNESCO Publishing, ParisGoogle Scholar
- Koyama Y, Kito M, Takii T, Saiki K, Tsukida K, Yamashita J (1982) Configuration of the carotenoid in the reaction centers of photosynthetic bacteria. Comparison of the resonance Raman spectrum of the reaction center of Rhodopseudomonas sphaeroides G1C with those of cis-trans isomers of β-carotene. Biochim Biophys Acta Bioenergy 680(2):109–118. https://doi.org/10.1016/0005-2728(82)90001-9 CrossRefGoogle Scholar
- Koyama Y, Takii T, Saiki K, Tsukida K (1983) Configuration of the carotenoid in the reaction centers of photosynthetic bacteria. 2: comparison of the resonance Raman lines of the reaction centers with those of the 14 different cis-trans isomers of β-carotene. Photobiochem Photobiophys 5:139–150Google Scholar
- Koyama Y, Takatsuka I, Nakata M, Tasumi M (1988) Raman and infrared spectra of the all-trans, 7-cis, 9-cis, 13-cis and 15-cis isomers of β-carotene: key bands distinguishing stretched or terminal-bent configurations form central-bent configurations. J Raman Spectrosc 19(1):37–49. https://doi.org/10.1002/jrs.1250190107 CrossRefGoogle Scholar
- Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In: Douce R, Packer L (eds) Methods in enzymology, vol 148. Academic Press, Amsterdam, pp 350–382Google Scholar
- Näveke A, Lapouge K, Sturgis JN, Hartwich G, Simonin I, Scheer H, Robert B (1997) Resonance Raman spectroscopy of metal-substituted bacteriochlorophylls: characterization of Raman bands sensitive to bacteriochlorin conformation. J Raman Spectrosc 28(8):599–604. https://doi.org/10.1002/(SICI)1097-4555(199708)28:8 CrossRefGoogle Scholar
- Pendon ZD, Sullivan JO, van der Hoef I, Lugtenburg J, Cua A, Bocian DF, Birge RR, Frank HA (2005) Stereoisomers of carotenoids: spectroscopic properties of locked and unlocked cis-isomers of spheroidene. Photosynth Res 86(1):5–24. https://doi.org/10.1007/s11120-005-1205-0 CrossRefPubMedGoogle Scholar
- Ruban AV, Berera R, Ilioaia C, van Stokkum IHM, Kennis JTM, Pascal AA, van Amerongen H, Robert B, Horton P, van Grondelle R (2007) Identification of a mechanism of photoprotective energy dissipation in higher plants. Nature 450(7169):575–578. https://doi.org/10.1038/nature06262 CrossRefPubMedGoogle Scholar
- Ševčíková T, Horák A, Klimeš V, Zbránková V, Demir-Hilton E, Sudek S, Jenkins J, Schmutz J, Přibyl P, Fousek J, Vlček Č, Lang BF, Oborník M, Worden AZ, Eliáš M (2015) Updating algal evolutionary relationships through plastid genome sequencing: did alveolate plastids emerge through endosymbiosis of an ochrophyte? Sci Rep. https://doi.org/10.1038/srep10134 PubMedPubMedCentralGoogle Scholar