Photosynthesis Research

, Volume 139, Issue 1–3, pp 367–385 | Cite as

Differential temperature effects on dissipation of excess light energy and energy partitioning in lut2 mutant of Arabidopsis thaliana under photoinhibitory conditions

  • Antoaneta V. PopovaEmail author
  • Konstantin Dobrev
  • Maya Velitchkova
  • Alexander G. Ivanov
Original Article


The high-light-induced alterations in photosynthetic performance of photosystem II (PSII) and photosystem I (PSI) as well as effectiveness of dissipation of excessive absorbed light during illumination for different periods of time at room (22 °C) and low (8–10 °C) temperature of leaves of Arabidopsis thaliana, wt and lut2, were followed with the aim of unraveling the role of lutein in the process of photoinhibition. Photosynthetic parameters of PSII and PSI were determined on whole leaves by PAM fluorometer and oxygen evolving activity—by a Clark-type electrode. In thylakoid membranes, isolated from non-illuminated and illuminated for 4.5 h leaves of wt and lut2 the photochemical activity of PSII and PSI and energy interaction between the main pigment–protein complexes was determined. Results indicate that in non-illuminated leaves of lut2 the maximum rate of oxygen evolution and energy utilization in PSII is lower, excitation pressure of PSII is higher and cyclic electron transport around PSI is faster than in wt leaves. Under high-light illumination, lut2 leaves are more sensitive in respect to PSII performance and the extent of increase of excitation pressure of PSII, ΦNO, and cyclic electron transport around PSI are higher than in wt leaves, especially when illumination is performed at low temperature. Significant part of the excessive light energy is dissipated via mechanism, not dependent on ∆pH and to functioning of xanthophyll cycle in LHCII, operating more intensively in lut2 leaves.


Arabidopsis thaliana lut2 mutant Photosynthetic performance High-light treatment Energy partitioning 





Cyclic electron flow around PSI


3-(3,4-dichlorophenyl)1,1-dimethyl urea




Ethylenediamine-tetraacetic acid


Electron transport rate


Minimum yield of chlorophyll fluorescence in open PSII centers


Maximal chlorophyll fluorescence in dark-adapted state


Maximal chlorophyll fluorescence in light-adapted state


Variable chlorophyll fluorescence


Effective quantum yield of PSII


Quantum yield of the regulated energy dissipation of PSII


Quantum yield of non-regulated energy dissipation of PSII


Maximum photochemical efficiency of PSII in the dark-adapted state


Light compensation point


Light-harvesting chlorophyll a/b-protein complex of PSII


Light-harvesting chlorophyll a/b-protein complex of PSI


2(N-morpholino)ethanesulfonic acid


Methyl viologen


Non-photochemical quenching


Reaction center chlorophyll of PSI


Oxidized form of PSI reaction center


Photon flux density




Photosystem I


Photosystem II


Primary and secondary electron-accepting quinone in PSII


N-tris[hydroxymethyl]methyl-2-aminoethanesulfonic acid


N-tris[hydroxymethyl]methyl glycine



This work was partially supported by Bulgarian-Swiss Research Program, Project IZEBZO-143169/1. The seeds of the wt and mutant lut2 of A. thaliana were a generous gift from Prof. R. Bassi.


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© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Antoaneta V. Popova
    • 1
    Email author
  • Konstantin Dobrev
    • 1
  • Maya Velitchkova
    • 1
  • Alexander G. Ivanov
    • 1
    • 2
  1. 1.Institute of Biophysics and Biomedical EngineeringBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of BiologyUniversity of Western OntarioLondonCanada

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