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Photosynthesis Research

, Volume 134, Issue 1, pp 71–82 | Cite as

Carbon use efficiencies and allocation strategies in Prochlorococcus marinus strain PCC 9511 during nitrogen-limited growth

  • Kristina Felcmanová
  • Martin Lukeš
  • Eva Kotabová
  • Evelyn Lawrenz
  • Kimberly H. Halsey
  • Ondřej PrášilEmail author
Original Article

Abstract

We studied cell properties including carbon allocation dynamics in the globally abundant and important cyanobacterium Prochlorococcus marinus strain PCC 9511 grown at three different growth rates in nitrogen-limited continuous cultures. With increasing nitrogen limitation, cellular divinyl chlorophyll a and the functional absorption cross section of Photosystem II decreased, although maximal photosynthetic efficiency of PSII remained unaltered across all N-limited growth rates. Chl-specific gross and net carbon primary production were also invariant with nutrient-limited growth rate, but only 20% of Chl-specific gross carbon primary production was retained in the biomass across all growth rates. In nitrogen-replete cells, 60% of the assimilated carbon was incorporated into the protein pool while only 30% was incorporated into carbohydrates. As N limitation increased, new carbon became evenly distributed between these two pools. While many of these physiological traits are similar to those measured in other algae, there are also distinct differences, particularly the lower overall efficiency of carbon utilization. The latter provides new information needed for understanding and estimating primary production, particularly in the nutrient-limited tropical oceans where P. marinus dominates phytoplankton community composition.

Keywords

Prochlorococcus marinus Cyanobacteria Primary production Nitrogen limitation Carbon allocation 

Notes

Acknowledgements

We wish to thank Jiří Šetlík for technical support, and Jana Hofhanzlová, Eva Žišková, Jason Dean, and Ondřej Komárek for their assistance. This work was supported by projects MSMT Kontakt II LH11064 (to O. P.), MSMT NPU I LO 1416 (to O. P.), and GAJU 143/2013/P (to K. F.).

Supplementary material

11120_2017_418_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Institute of MicrobiologyCzech Academy of SciencesTřeboňCzech Republic
  2. 2.Department of Experimental Plant Biology, Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic
  3. 3.Department of MicrobiologyOregon State UniversityCorvallisUSA

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