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Photosynthesis Research

, Volume 133, Issue 1–3, pp 215–223 | Cite as

Membrane fluidity controls redox-regulated cold stress responses in cyanobacteria

  • Eugene G. Maksimov
  • Kirill S. Mironov
  • Marina S. Trofimova
  • Natalya L. Nechaeva
  • Daria A. Todorenko
  • Konstantin E. Klementiev
  • Georgy V. Tsoraev
  • Eugene V. Tyutyaev
  • Anna A. Zorina
  • Pavel V. Feduraev
  • Suleyman I. Allakhverdiev
  • Vladimir Z. Paschenko
  • Dmitry A. Los
Original Article

Abstract

Membrane fluidity is the important regulator of cellular responses to changing ambient temperature. Bacteria perceive cold by the transmembrane histidine kinases that sense changes in thickness of the cytoplasmic membrane due to its rigidification. In the cyanobacterium Synechocystis, about a half of cold-responsive genes is controlled by the light-dependent transmembrane histidine kinase Hik33, which also partially controls the responses to osmotic, salt, and oxidative stress. This implies the existence of some universal, but yet unknown signal that triggers adaptive gene expression in response to various stressors. Here we selectively probed the components of photosynthetic machinery and functionally characterized the thermodynamics of cyanobacterial photosynthetic membranes with genetically altered fluidity. We show that the rate of oxidation of the quinone pool (PQ), which interacts with both photosynthetic and respiratory electron transport chains, depends on membrane fluidity. Inhibitor-induced stimulation of redox changes in PQ triggers cold-induced gene expression. Thus, the fluidity-dependent changes in the redox state of PQ may universally trigger cellular responses to stressors that affect membrane properties.

Keywords

Cyanobacteria Desaturase Fatty acids Fluidity Fluorescence Membrane Lipids Plastoquinone pool Photosystem II Photosystem I Redox regulation 

Abbreviations

ETC

Electron transport chain

DCMU

(3-(3,4-dichlorophenyl)-1,1-dimethylurea)

DBMIB

Dibromothymoquinone

FA

Fatty acid

FAD

Fatty acid desaturase

FI

Fluorescence induction

MR

Modulated reflection

PQ

Plastoquinone pool

UFA

Unsaturated fatty acid

Notes

Acknowledgements

This work was supported by the grant from Russian Science Foundation (14-24-00020) to D.A.L. E.G.M. was supported by a grant from Russian Foundation for Basic Research (No. 15-04-01930a), Russian Ministry of Education and Science (project MK-5949.2015.4), and by RFBR and Moscow city Government according to the research project no. 15-34-70007 «mol_а_mos». P.V.F. was supported by a grant from Russian Foundation for Basic Research (No. 16-34-50175 mol_nr). S.I.A. was supported by Russian Science Foundation (Grant No. 14-14-00039).

Supplementary material

11120_2017_337_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 16 KB)
11120_2017_337_MOESM2_ESM.pptx (436 kb)
Supplementary material 2 (PPTX 436 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Eugene G. Maksimov
    • 1
  • Kirill S. Mironov
    • 2
  • Marina S. Trofimova
    • 2
  • Natalya L. Nechaeva
    • 3
  • Daria A. Todorenko
    • 1
  • Konstantin E. Klementiev
    • 1
  • Georgy V. Tsoraev
    • 1
  • Eugene V. Tyutyaev
    • 4
  • Anna A. Zorina
    • 2
  • Pavel V. Feduraev
    • 2
    • 5
  • Suleyman I. Allakhverdiev
    • 2
  • Vladimir Z. Paschenko
    • 1
  • Dmitry A. Los
    • 2
  1. 1.Department of Biophysics, Faculty of BiologyM.V. Lomonosov Moscow State UniversityMoscowRussia
  2. 2.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  3. 3.Chemical Enzymology Department, Faculty of ChemistryM.V. Lomonosov Moscow State UniversityMoscowRussia
  4. 4.Department of Biotechnology, Bioengineering and Biochemistry, Faculty Biotechnology and BiologyOgarev Mordovia State UniversitySaranskRussia
  5. 5.Chemical-Biological InstituteImmanuel Kant Federal Baltic UniversityKaliningradRussia

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