Photosynthesis Research

, Volume 131, Issue 3, pp 255–266 | Cite as

Modular antenna of photosystem I in secondary plastids of red algal origin: a Nannochloropsis oceanica case study

  • David Bína
  • Zdenko Gardian
  • Miroslava Herbstová
  • Radek Litvín
Original Article

Abstract

Photosystem I (PSI) is a multi-subunit integral pigment–protein complex that performs light-driven electron transfer from plastocyanin to ferredoxin in the thylakoid membrane of oxygenic photoautotrophs. In order to achieve the optimal photosynthetic performance under ambient irradiance, the absorption cross section of PSI is extended by means of peripheral antenna complexes. In eukaryotes, this role is played mostly by the pigment–protein complexes of the LHC family. The structure of the PSI-antenna supercomplexes has been relatively well understood in organisms harboring the primary plastid: red algae, green algae and plants. The secondary endosymbiotic algae, despite their major ecological importance, have so far received less attention. Here we report a detailed structural analysis of the antenna-PSI association in the stramenopile alga Nannochloropsis oceanica (Eustigmatophyceae). Several types of PSI-antenna assemblies are identified allowing for identification of antenna docking sites on the PSI core. Instances of departure of the stramenopile system from the red algal model of PSI-Lhcr structure are recorded, and evolutionary implications of these observations are discussed.

Keywords

Photosystem I Electron microscopy Light-harvesting complex Nannochloropsis Stramenopila 

Abbreviations

Chl

Chlorophyll

N.

Nannochloropsis

P700

The primary donor of the photosystem I reaction center

PS

Photosystem

PMS

Phenazine methosulfate

RC

Reaction center

IsiA

Outer antenna of PSI in cyanobacteria

Pcb

Outer antenna of PSI in chlorophyll b-containing cyanobacteria

Supplementary material

11120_2016_315_MOESM1_ESM.pdf (2.5 mb)
Supplementary material 1 (PDF 2565 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Institute of Plant Molecular BiologyBiology Centre CASČeské BudějoviceCzech Republic
  2. 2.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic

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