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Photosynthesis Research

, Volume 130, Issue 1–3, pp 445–467 | Cite as

Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086”

  • Hirohisa Komatsu
  • Katsuhiro Wada
  • Terumitsu Kanjoh
  • Hideaki Miyashita
  • Mayumi Sato
  • Masanobu Kawachi
  • Masami KobayashiEmail author
Review

Abstract

In this review, we introduce our recent studies on divinyl chlorophylls functioning in unique marine picoplankton Prochlorococcus sp. (1) Essential physicochemical properties of divinyl chlorophylls are compared with those of monovinyl chlorophylls; separation by normal-phase and reversed-phase high-performance liquid chromatography with isocratic eluent mode, absorption spectra in four organic solvents, fluorescence information (emission spectra, quantum yields, and life time), circular dichroism spectra, mass spectra, nuclear magnetic resonance spectra, and redox potentials. The presence of a mass difference of 278 in the mass spectra between [M+H]+ and the ions indicates the presence of a phytyl tail in all the chlorophylls. (2) Precise high-performance liquid chromatography analyses show divinyl chlorophyll a’ and divinyl pheophytin a as the minor key components in four kinds of Prochlorococcus sp.; neither monovinyl chlorophyll a′ nor monovinyl pheophytin a is detected, suggesting that the special pair in photosystem I and the primary electron acceptor in photosystem II are not monovinyl but divinyl-type chlorophylls. (3) Only Prochlorococcus sp. NIES-2086 possesses both monovinyl chlorophyll b and divinyl chlorophyll b, while any other monovinyl-type chlorophylls are absent in this strain. Monovinyl chlorophyll b is not detected at all in the other three strains. Prochlorococcus sp. NIES-2086 is the first example that has both monovinyl chlorophyll b as well as divinyl chlorophylls a/b as major chlorophylls.

Keywords

Chlorophyll Divinyl chlorophyll Pheophytin Photosynthesis Prochlorococcus 

Abbreviations

CAO

Chlorophyllide a oxygenase

CD

Circular dichroism

Chl

Chlorophyll

DV-Chl

Divinyl chlorophyll

DV-Phe

Divinyl pheophytin

DVR

Divinyl reductase

HMBC

Heteronuclear multiple-bond correlation

HSQC

Heteronuclear single quantum coherence

HPLC

High-performance liquid chromatography

MV-Chl

Monovinyl chlorophyll

MV-Phe

Monovinyl pheophytin

NMR

Nuclear magnetic resonance

NOESY

Nuclear Overhauser and exchange spectroscopy

Phe

Pheophytin

PS

Photosystem

RC

Reaction center

Notes

Acknowledgments

We thank Dr. Mayumi Ohnishi-Kameyama, Dr. Hiroshi Ono (National Food Research Institute), Dr. Hiroyuki Koike (Chuo University), Messrs. Yuhta Isei, Taku Kaitani, Dr. Yutaka Hanawa, and Dr.Yoshihiro Shiraiwa (Univ. Tsukuba) for their kind and invaluable help.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Hirohisa Komatsu
    • 1
  • Katsuhiro Wada
    • 1
  • Terumitsu Kanjoh
    • 1
  • Hideaki Miyashita
    • 2
  • Mayumi Sato
    • 3
  • Masanobu Kawachi
    • 3
  • Masami Kobayashi
    • 1
    Email author
  1. 1.Division of Materials Science, Faculty of Pure and Applied ScienceUniversity of TsukubaTsukubaJapan
  2. 2.Graduate School of Human and Environment StudiesKyoto UniversityKyotoJapan
  3. 3.National Institute for Environmental StudiesTsukubaJapan

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