Probing the nucleotide-binding activity of a redox sensor: two-component regulatory control in chloroplasts
- 1.4k Downloads
Two-component signal transduction systems mediate adaptation to environmental changes in bacteria, plants, fungi, and protists. Each two-component system consists of a sensor histidine kinase and a response regulator. Chloroplast sensor kinase (CSK) is a modified sensor histidine kinase found in chloroplasts—photosynthetic organelles of plants and algae. CSK regulates the transcription of chloroplast genes in response to changes in photosynthetic electron transport. In this study, the full-length and truncated forms of Arabidopsis CSK proteins were overexpressed and purified in order to characterise their kinase and redox sensing activities. Our results show that CSK contains a modified kinase catalytic domain that binds ATP with high affinity and forms a quinone adduct that may confer redox sensing activity.
KeywordsTwo-component regulatory system Sensor histidine kinase Response regulator Chloroplast sensor kinase Plastoquinone Redox regulation Chloroplast DNA Transcription Cyanobacteria Photosystem stoichiometry
Photosynthesis converts light energy from the sun into useful chemical energy. This important biological process takes place in some prokaryotes and in chloroplasts—bioenergetic organelles of eukaryotic plants and algae. In photosynthesis, in chloroplasts and cyanobacteria, light-driven primary electron transfer is carried out by the photochemical reaction centres of two photosystems, photosystem II (PS II) and photosystem I (PS I). A mobile electron carrier—plastoquinone (PQ)—in the thylakoid membrane is a link in the electron transport chain that connects these two reaction centres in series. The reduction–oxidation (redox) state of the pool of PQ molecules determines distribution of excitation energy between PS II and PS I by controlling the reversible phosphorylation of polypeptides of light-harvesting complex II (LHC II) in chloroplasts (Allen 1992; Allen et al. 1981). The redox state of the PQ pool also controls transcription of chloroplast DNA, regulating expression of genes that encode reaction-centre proteins of PS II and PS I, thus initiating a long-term light quality acclimatory process known as photosystem stoichiometry adjustment (Pfannschmidt et al. 1999). In cyanobacteria, prokaryotes from which chloroplasts originated, a similar redox control of photosystem stoichiometry is observed (Fujita 1997; Murakami et al. 1997).
Redox chemistry in the thylakoid membrane is coupled to chloroplast DNA transcription by a bacterial-type two-component signal transduction system (TCS). TCSs are the predominant signalling mechanisms in prokaryotic organisms. TCSs consist of two proteins, a sensor histidine kinase (component 1) and a response regulator (component 2) (Stock et al. 2000). Chloroplasts contain a modified bacterial-type sensor histidine kinase, named chloroplast sensor kinase (CSK) (Puthiyaveetil et al. 2008). In Arabidopsis, CSK is encoded by the nuclear gene At1g67840 and imported into chloroplasts as a precursor protein synthesised in the cytosol. Under changing light quality conditions that perturb the redox state of the PQ pool, Arabidopsis plants lacking the CSK gene are unable to regulate transcription of the chloroplast reaction-centre gene psaA (encoding the PsaA protein of photosystem I), and are impaired in photosystem stoichiometry adjustments (Puthiyaveetil et al. 2008). CSK is therefore suggested as the sensor component in the signal transduction chain that underlies photosystem stoichiometry adjustments (Puthiyaveetil et al. 2008). The mechanisms by which the photosynthetic electron transport chain controls the activity of CSK, and how CSK regulates the transcription of the psaA gene, are under investigation (Puthiyaveetil et al. 2013). Here, we report on the ATP-binding activity of CSK using the overexpressed, purified full-length recombinant CSK protein. We show that the CSK has a modified ATP-binding site and yet it binds ATP with a K d value similar to that of unmodified histidine kinases.
Materials and methods
Secondary structure prediction
The secondary structure of CSK was predicted using the PSIPRED programme (http://bioinf.cs.ucl.ac.uk/psipred/).
Construction of recombinant plasmids
Primers used for cloning full-length CSK (CSK_F) and truncated CSK (CSK_T). Sequences in lower case are restriction site overhangs
Expression and purification of recombinant CSK protein
Recombinant plasmids were transformed into BL21(DE3) chemically competent cells (stratagene). Transformed bacterial colonies grown on agar plates were used to inoculate starter cultures (20 ml each) in Luria broth (LB) growth medium (8) supplemented with 100 μg mL−1 ampicillin as the selection agent. Each culture was grown overnight, then diluted 1:100 in 2 L LB media and grown at 37 °C to an optical density of ~0.55 at 600 nm before inducing protein expression by addition of isopropyl β-D-1-thiogalactopyranoside (IPTG) (Melford) to a final concentration of 100 µm. Bacterial cultures were grown for a further 16 h at 17 °C. Cells were harvested by centrifugation at 6000 rpm for 10 min. The pellet was re-suspended in 20 mL of resuspension buffer (300 mM NaCl, 50 mm NaH2PO4 pH 7.4, 25 mm imidazole, and 1 mm PMSF) and the cells lysed with an EmulsiFlex-C3 homogenizer (Avestin). Lysate was separated by centrifugation at 18,000 rpm for 20 min. The supernatant was applied to a Ni2+ affinity chromatography column (GE Healthcare) and the N-terminally poly-histidine tagged CSK protein was purified according to the column manufacturer’s instructions. For ATP-binding assay, the elution buffer in the purified protein was exchanged with ATP-binding buffer (10 mm NaCl and 10 mm tris–HCl (pH 8)) using the PD-10 desalting column (Amersham Biosciences).
In vitro autophosphorylation assay in the presence of redox agents
Recombinant CSK protein at 2.5 µm concentration was mixed with the kinase reaction buffer (50 mm tris–HCl (pH 7.5), 50 mm KCl, 10 % glycerol, and 10 mm MgCl2) and the following redox agents: 2 mm K3Fe(CN)6, 6 mm DTT, 0.5 mm benzoquinone, and 0.5 mm hydroquinone in a final volume of 25 µL and incubated at room temperature for 30 min. Autophosphorylation was initiated by the addition of 5 µL of 5-fold concentrated ATP solution giving a final concentration of 0.5 mm ATP and a specific activity of 5 µCi [γ-32P]ATP (6000 Ci mmol−1) (Perkin-Elmer). Reactions proceeded for 60 min at 30 °C. The autophosphorylation reaction was stopped by addition of 6 µl of 5X Laemmli sample buffer (Laemmli 1970). Proteins were resolved on a 12 % SDS-PAGE gel, blotted onto a PVDF membrane and the 32P-labeling analysed by a phosphor screen.
TNP-ATP-binding assay was carried out in a total volume of 3 mL ATP-binding buffer containing 2 µm of CSK_T and 1 µm of TNP-ATP. Samples were prepared in a 1 cm X 4 cm quartz cuvette. Fluorescence measurement was carried out using Perkin-Elmer LS55 spectrofluorometer with the excitation wavelength set at 410 nm and the emission wavelength at 500–650 nm. Excitation and emission monochromators were at 5 nm and 10 nm bandwidth, respectively. TNP-ATP was excited at 410 nm and the fluorescence emission was scanned in a wavelength range of 500–650 nm.
The TNP-ATP-binding titration was carried out by successive addition of varying concentration of TNP-ATP to 2 µm CSK_T diluted in ATP-binding buffer. Control titration without protein was performed in the same way. The fluorescence intensity increase at 550 nm was recorded. Subtracting the control fluorescence value from the sample value corrected for the buffer-TNP-ATP fluorescence. The total volume of TNP-ATP was less than 0.001 % of the total sample volume.
TNP-ATP displacement was carried out by successive addition of varying concentrations of ATP to a sample containing 2 µm CSK_T, 1 µm TNP-ATP in the ATP-binding buffer. Decrease in fluorescence emission intensity at 550 nm was monitored. The total volume of ATP added was less than 0.05 % of total sample volume.
Secondary structure prediction of the ATP-lid of CSK
CSK contains a modified CA domain
Recombinant protein production
CSK does not autophosphorylate in vitro
CSK binds an ATP analogue TNP-ATP
TNP-ATP dissociation constant
Sensor histidine kinase
The overall structure of the CA domain of the histidine kinase is formed of a α/β sandwich fold, composed of three helices and five stranded β-sheets, which is distinct from that of serine/threonine or tyrosine kinases. However, the histidine kinase fold bears strong similarity to the ATP-binding domain of DNA topoisomerase II (Roca and Wang 1992), DNA Gyrase B (Ali et al. 1995), the chaperone Hsp90 (Panaretou et al. 1998), and the DNA repair enzyme MutL (Ban et al. 1999). The CA domain is characteristic of its conserved sequence motifs: N, F, G1, G2, and G3 boxes that are important for stabilisation of an ATP molecule and for the hydrolysis of the ATP γ-phosphate. The N-box and G1-box are involved in the stabilisation of the adenine ring of ATP. In particular, a conserved aspartic residue found within the G1-box forms a hydrogen bond with the amino N6 of the adenine base (Marina et al. 2001; Trajtenberg et al. 2010). This interaction is crucial for conferring specificity to ATP and to prevent the binding of other nucleotides such as GTP. The CA domains discriminate nucleotides such as GTP based on the fact that they lack the amino group required for forming a hydrogen bond with the carboxyl group of the conserved aspartic acid within the G1-box. The F-box, which is the less conserved region of CA domain, and the G2-box together form a flexible loop that acts as the ATP-lid and controls the entry of the ATP-Mg2+ complex and the release of the ADP-Mg2+ complex. Conserved glycine residues in the G2-box facilitate the flexibility of the ATP-lid (Marina et al. 2001). The G1-box is fully conserved in all CSKs. The G2-box, however, is less conserved in chloroplast CSKs and unrecognisable in higher plants (Fig. 1). Nonetheless, this did not affect their ATP-binding activity. In contrast to higher plant CSK, substitution of any of the glycine residues in the G1 or G2 boxes of cyanobacterial CSK results in an inactive kinase (Ibrahim et al. 2016) suggesting that the modified CSKs contain a different catalytic fold.
CSK homologue in cyanobacteria is able to autophosphorylate in vitro and transfer phosphoryl groups to its response regulators Rre1 and RppA. Furthermore, its autokinase activity can be inhibited by sodium ions (Ibrahim et al. 2016). Although the modified CSK binds ATP in vitro (Fig. 5), we could not detect its autophosphorylation (Fig. 4). This could be because of a number of reasons. Firstly, plant CSK could be autokinase inactive at its ground state and thus a signal may be required to activate it. In our assay, this signal may have been absent. Secondly, the CSK might require a cofactor(s) such as FAD for its redox sensing activity and that the recombinant and purified protein used in this study could be an incomplete apoenzyme. A third possibility is that the bacterially expressed recombinant CSK is not folded correctly for the kinase reaction. CSK may well have lost its ability to autophosphorylate and might function in a manner similar to some of the modified bacterial histidine kinases such as DivL. In contrast to DivL, however, CSK exhibits an ATP-binding affinity similar to prototypical histidine kinases, while DivL binds ATP analogue TNP-ATP poorly, with a K d value of 57 µm (Childers et al. 2014). The strong binding affinity of CSK for ATP thus indicates that it has retained its kinase activity.
The lack of a demonstrable autokinase activity in CSK also suggests that the modified CSKs have a catalytic mechanism that differs from that of their cyanobacterial homologues. Perhaps this modification is necessary for CSK to acquire a new substrate, as its cognate response regulators Ycf29 and Ycf27 are missing from chloroplasts in the green lineages (Puthiyaveetil and Allen 2009). Indeed, Arabidopsis CSK protein interacts with plastid transcription machineries, including sigma factor 1 (SIG1) (Puthiyaveetil et al. 2013, 2010), a transcriptional initiation factor that has a prokaryotic origin. SIG1 is required to initiate psbA and psaAB transcription (Shimizu et al. 2010).
Histidine kinases do not transfer the γ-phosphate group directly from an ATP molecule to their substrate. They instead use the high energy of the phosphoramide bond of the phospho-histidine to facilitate the transfer of the phosphate to an aspartate residue of the response regulator. Phospho-serine and -threonine are, however, thermodynamically more stable than phosphoramide or acyl-phosphate, they therefore cannot passively transfer phosphate groups. Indeed, several modified histidine kinases, such as ETR2 (Moussatche and Klee 2004), plant phytochromes (Fankhauser et al. 1999), α-ketoacid dehydrogenase kinase (Lasker et al. 2002), and pyruvate dehydrogenase kinase (PDK) (Thelen et al. 2000) have lost their ability to catalyse His–Asp phosphotransfer. They have instead acquired a catalytic mechanism that is similar to that of serine/threonine kinases in that they now phosphorylate their substrates on serine or threonine residues. It is likely that the modified CSKs in green lineage also acquired a serine/threonine-type catalytic mechanism similar to that of modified histidine kinases, a property that is yet to be demonstrated experimentally.
The PQ pool has a standard midpoint potential of +50 mV, n = 2 (Silverstein et al. 1993b) and an effective redox potential that changes with fluctuating light distribution between the photosystems. These fluctuations may affect a specific redox sensor. For example, the redox state of the PQ pool controls the light-dependent phosphorylation of chloroplast light-harvesting complex II (LHC II) (Allen 1992; Allen et al. 1981). The LHC II kinase (STN7/STT7) is responsible for phosphorylation of LHC II (Bellafiore et al. 2005; Depege et al. 2003; Rochaix 2007). LHC II kinase has a midpoint potential of +48 mV, which is similar to that of PQ pool midpoint potential (Silverstein et al. 1993a). Some quinone pool sensors, however, do not utilise its redox signal. RegB, for example, interacts with the reduced and oxidised forms of ubiquinone with similar affinities, and only the oxidised form of the ubiquinone seems to switch-off the autophosphorylation of RegB (Wu and Bauer 2010). The autophosphorylation of RegB is, in fact, regulated through an allosteric effect triggered by binding of ubiquinone/ubiquinol rather than oxidation by ubiquinone (Wu and Bauer 2010). The UV absorption spectra and the primary amino acid sequence of CSK (results not shown) did not reveal potential spectral signatures or binding motifs characteristic of redox sensitive cofactors such as heme or flavin, which suggests that CSK may employ a RegB-type quinone sensing mechanism rather than taking part in direct redox sensing. We also noted that benzoquinone-treated and hydroquinone-treated CSK protein migrated as two bands on the SDS-PAGE gel that is both denaturing and reducing (Fig. 4, lanes 4 and 5). Cysteine residue in enzymes are known to react with quinones to form quinone-cysteine adducts that cannot be broken with reducing agents, for example by DTT used in our sample buffer (Li et al. 2005). Cysteine residues in CSK could be forming a thioether-quinone adduct. This observation further strengthens the quinone-binding activity of CSK. However, it is not yet clear whether the adduct formation still occurs with the plastoquinone in the thylakoid membrane.
One question that is still unclear is how a water-soluble redox sensor such as CSK sense the quinone redox signal located within the thylakoid membrane? PQ is a mobile electron carrier that shuttles electrons between PS II and cytochrome b 6 f in the thylakoid membrane. For membrane-anchored redox sensors, quinone appears to be an attractive candidate as the signalling molecule; however, for soluble redox sensors, it is a more challenging prospect. Nevertheless, there are several soluble proteins that are quinone pool sensors. The NifL sensor protein regulates the N2-fixation (nif) genes in Azotobacter vinelandii (Grabbe and Schmitz 2003). NifL is a flavoprotein that contains a redox active FAD cofactor that is reduced by the quinone pool. The CikA (circadian input kinase A) sensor protein from Synechococcus elongatus PCC 7942 is a soluble protein that senses the PQ pool unaided by a redox-active prosthetic group (Ivleva et al. 2006). In order for CSK to sense the PQ pool, it must have the ability to associate itself with the thylakoid membrane and it must possess a binding site for PQ. For CSK, the ability to associate with the thylakoid membrane may be imparted by the amphipathic helices in its GAF sensor domain. Can the GAF domain in CSK also act as a binding pocket for the reactive, hydrophobic PQ head group? GAF domains are known for their ability to bind small cofactors such as heme and nucleotides (Sardiwal et al. 2005). CSK, indeed, binds DBMIB with a K d value of 3.66 µm (Puthiyaveetil et al. 2013), a binding affinity comparable to other quinone binding proteins and thus strengthening the quinone-sensing activity of CSK.
Based on results presented here, we conclude that the catalytic domain of CSK became modified in evolution in order to accommodate a Ser/Thr kinases-type catalytic mechanism that was essential for its incorporation into an existing signal transduction network. CSK’s ATP-binding activity and ability to form quinone adducts further strengthen its role in the crucial sensory circuit that connects the redox state of the PQ pool with the chloroplast gene transcription, consistent with the CoRR hypothesis for the evolutionary retention of organellar genomes (Allen 1993a, b, 2003, 2015).
IMI thanks Queen Mary University of London for a graduate teaching studentship. SP held a Leverhulme Trust early career postdoctoral research fellowship. JFA acknowledges the support of research Grant F/07 476/AQ and fellowship EM-2015-068 of The Leverhulme Trust.
- Childers WS, Xu QP, Mann TH, Mathews II, Blair JA, Deacon AM, Shapiro L (2014) Cell fate regulation governed by a repurposed bacterial histidine kinase. Plos Biol 12Google Scholar
- Ibrahim IM, Puthiyaveetil S, Allen JF (2016) A two-component regulatory system in transcriptional control of photosystem stoichiometry: redox-dependent and sodium ion-dependent phosphoryl transfer from cyanobacterial histidine kinase Hik2 to response regulators Rre1 and RppA. Frontiers in plant science 7 doi: 10.3389/fpls.2016.00137
- Motulsky H, Christopoulos A (2003) Fitting models to biological data using linear and nonlinear regression. A practical guide to curve fitting. GraphPad Software Inc, http://www.graphpad.com, San Diego, CA
- Puthiyaveetil S, Ibrahim IM, Allen JF (2013) Evolutionary rewiring: a modified prokaryotic gene regulatory pathway in chloroplasts. Philos T R Soc B 367 doi: 10.1098/rstb20120260
- Wu JA, Bauer CE (2010) RegB kinase activity is controlled in part by monitoring the ratio of oxidized to reduced ubiquinones in the ubiquinone pool. Mbio 1Google Scholar
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.