Photosynthesis Research

, Volume 130, Issue 1–3, pp 11–17 | Cite as

Synechocystis mutants defective in manganese uptake regulatory system, ManSR, are hypersensitive to strong light

  • Anna Zorina
  • Maria A. Sinetova
  • Elena V. Kupriyanova
  • Kirill S. Mironov
  • Irina Molkova
  • Lyudmila V. Nazarenko
  • Vladislav V. Zinchenko
  • Dmitry A. Los
Original Article


High affinity transport of manganese ions (Mn2+) in cyanobacteria is carried by an ABC-type transporter, encoded by the mntCAB operon, which is derepressed by the deficiency of Mn2+. Transcription of this operon is negatively regulated by the two-component system consisting of a sensory histidine kinase ManS and DNA-binding response regulator ManR. In this study, we examined two Synechocystis mutants, defective in ManS and ManR. These mutants were unable to grow on high concentrations of manganese. Furthermore, they were sensitive to high light intensity and unable to recover after short-term photoinhibition. Under standard illumination and Mn2+ concentration, mutant cells revealed the elevated levels of transcripts of genes involved in the formation of Photosystem II (psbA, psbD, psbC, pap-operon). This finding suggests that, in mutant cells, the PSII is sensitive to high concentrations of Mn2+ even at relatively low light intensity.


Synechocystis Cyanobacteria Ions Manganese Sensors Stress Transcription factor Transporter 



Histidine kinase


Response regulator


Open reading frame


Photosystem II


Reaction center



This work was supported by grants from Russian Science Foundation (no. 04-24-00020) to DAL and from Russian Foundation for Basic Research (13-04-01767) to VVZ.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Anna Zorina
    • 1
  • Maria A. Sinetova
    • 1
  • Elena V. Kupriyanova
    • 1
  • Kirill S. Mironov
    • 1
  • Irina Molkova
    • 1
  • Lyudmila V. Nazarenko
    • 2
  • Vladislav V. Zinchenko
    • 3
  • Dmitry A. Los
    • 1
  1. 1.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Moscow City Teacher Training UniversityMoscowRussia
  3. 3.Department of Genetics, Faculty of BiologyMoscow State UniversityMoscowRussia

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