Photosynthesis Research

, Volume 130, Issue 1–3, pp 11–17 | Cite as

Synechocystis mutants defective in manganese uptake regulatory system, ManSR, are hypersensitive to strong light

  • Anna Zorina
  • Maria A. Sinetova
  • Elena V. Kupriyanova
  • Kirill S. Mironov
  • Irina Molkova
  • Lyudmila V. Nazarenko
  • Vladislav V. Zinchenko
  • Dmitry A. Los
Original Article

Abstract

High affinity transport of manganese ions (Mn2+) in cyanobacteria is carried by an ABC-type transporter, encoded by the mntCAB operon, which is derepressed by the deficiency of Mn2+. Transcription of this operon is negatively regulated by the two-component system consisting of a sensory histidine kinase ManS and DNA-binding response regulator ManR. In this study, we examined two Synechocystis mutants, defective in ManS and ManR. These mutants were unable to grow on high concentrations of manganese. Furthermore, they were sensitive to high light intensity and unable to recover after short-term photoinhibition. Under standard illumination and Mn2+ concentration, mutant cells revealed the elevated levels of transcripts of genes involved in the formation of Photosystem II (psbA, psbD, psbC, pap-operon). This finding suggests that, in mutant cells, the PSII is sensitive to high concentrations of Mn2+ even at relatively low light intensity.

Keywords

Synechocystis Cyanobacteria Ions Manganese Sensors Stress Transcription factor Transporter 

Abbreviations

Hik

Histidine kinase

Rre

Response regulator

ORF

Open reading frame

PS II

Photosystem II

RC

Reaction center

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Anna Zorina
    • 1
  • Maria A. Sinetova
    • 1
  • Elena V. Kupriyanova
    • 1
  • Kirill S. Mironov
    • 1
  • Irina Molkova
    • 1
  • Lyudmila V. Nazarenko
    • 2
  • Vladislav V. Zinchenko
    • 3
  • Dmitry A. Los
    • 1
  1. 1.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.Moscow City Teacher Training UniversityMoscowRussia
  3. 3.Department of Genetics, Faculty of BiologyMoscow State UniversityMoscowRussia

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