Synechocystis mutants defective in manganese uptake regulatory system, ManSR, are hypersensitive to strong light
- 437 Downloads
High affinity transport of manganese ions (Mn2+) in cyanobacteria is carried by an ABC-type transporter, encoded by the mntCAB operon, which is derepressed by the deficiency of Mn2+. Transcription of this operon is negatively regulated by the two-component system consisting of a sensory histidine kinase ManS and DNA-binding response regulator ManR. In this study, we examined two Synechocystis mutants, defective in ManS and ManR. These mutants were unable to grow on high concentrations of manganese. Furthermore, they were sensitive to high light intensity and unable to recover after short-term photoinhibition. Under standard illumination and Mn2+ concentration, mutant cells revealed the elevated levels of transcripts of genes involved in the formation of Photosystem II (psbA, psbD, psbC, pap-operon). This finding suggests that, in mutant cells, the PSII is sensitive to high concentrations of Mn2+ even at relatively low light intensity.
KeywordsSynechocystis Cyanobacteria Ions Manganese Sensors Stress Transcription factor Transporter
Open reading frame
- PS II
This work was supported by grants from Russian Science Foundation (no. 04-24-00020) to DAL and from Russian Foundation for Basic Research (13-04-01767) to VVZ.
- Brandt AM, Raksajit W, Mulo P, Incharoensakdi A, Salminen TA, Mäenpää P (2009) Transcriptional regulation and structural modeling of the FutC subunit of an ABC-type iron transporter in Synechocystis sp. strain PCC 6803. Arch Microbiol 191:561–570. doi: 10.1007/s00203-009-0482-y PubMedCrossRefGoogle Scholar
- Hernández-Prieto MA, Schön V, Georg J, Barreira L, Varela J, Hess WR, Futschik ME (2012) Iron deprivation in Synechocystis: inference of pathways, non-coding RNAs, and regulatory elements from comprehensive expression profiling. G3 2:1475–1495. doi: 10.1534/g3.112.003863 PubMedPubMedCentralCrossRefGoogle Scholar
- Larson EJ, Pecoraro VL (1992) Introduction to manganese enzymes. In: Pecoraro VL (ed) Manganese redox enzymes. VCH Publishers Inc., New York, pp 1–28Google Scholar
- Mironov KS, Los DA (2015) RNA isolation from Synechocystis. Bio-protocol 5:e1428. http://www.bio-protocol.org/e1428
- Sharon S, Salomon E, Kranzler C, Lis H, Lehmann R, Georg J, Zer H, Hess WR, Keren N (2014) The hierarchy of transition metal homeostasis: iron controls manganese accumulation in a unicellular cyanobacterium. Biochim Biophys Acta 1837:1990–1997. doi: 10.1016/j.bbabio.2014.09.007 PubMedCrossRefGoogle Scholar
- Wegener KM, Welsh EA, Thornton LE, Keren N, Jacobs JM, Hixson KK, Monroe ME, Camp DG 2nd, Smith RD, Pakrasi HB (2008) High sensitivity proteomics assisted discovery of a novel operon involved in the assembly of photosystem II, a membrane protein complex. J Biol Chem 283:27829–27837. doi: 10.1074/jbc.M803918200 PubMedCrossRefGoogle Scholar
- Yamaguchi K, Suzuki I, Yamamoto H, Lyukevich AA, Bodrova I, Los DA, Piven I, Zinchenko V, Kanehisa M, Murata N (2002) Two-component Mn2+-sensing system negatively regulates expression of the mntCAB operon in Synechocystis. Plant Cell 14:2901–2913. doi: 10.1105/tpc.006262 PubMedPubMedCentralCrossRefGoogle Scholar
- Zavřel T, Sinetova MA, Červený J (2015) Measurement of chlorophyll a and carotenoid concentration in cyanobacteria. Bio-protocol 5:e1467. http://www.bio-protocol.org/e1467