Photosynthesis Research

, Volume 124, Issue 3, pp 315–335 | Cite as

Electronic coupling of the phycobilisome with the orange carotenoid protein and fluorescence quenching

  • Igor N. Stadnichuk
  • Pavel M. Krasilnikov
  • Dmitry V. Zlenko
  • Alexandra Ya. Freidzon
  • Mikhail F. Yanyushin
  • Andrei B. Rubin
Regular Paper

Abstract

Using computational modeling and known 3D structure of proteins, we arrived at a rational spatial model of the orange carotenoid protein (OCP) and phycobilisome (PBS) interaction in the non-photochemical fluorescence quenching. The site of interaction is formed by the central cavity of the OCP monomer in the capacity of a keyhole to the characteristic external tip of the phycobilin-containing domain (PB) and folded loop of the core-membrane linker LCM within the PBS core. The same central protein cavity was shown to be also the site of the OCP and fluorescence recovery protein (FRP) interaction. The revealed geometry of the OCP to the PBLCM attachment is believed to be the most advantageous one as the LCM, being the major terminal PBS fluorescence emitter, gathers, before quenching by OCP, the energy from most other phycobilin chromophores of the PBS. The distance between centers of mass of the OCP carotenoid 3′-hydroxyechinenone (hECN) and the adjacent phycobilin chromophore of the PBLCM was determined to be 24.7 Å. Under the dipole–dipole approximation, from the point of view of the determined mutual orientation and the values of the transition dipole moments and spectral characteristics of interacting chromophores, the time of the direct energy transfer from the phycobilin of PBLCM to the S1 excited state of hECN was semiempirically calculated to be 36 ps, which corresponds to the known experimental data and implies the OCP is a very efficient energy quencher. The complete scheme of OCP and PBS interaction that includes participation of the FRP is proposed.

Keywords

Allophycocyanin Fluorescence LCM–polypeptide Orange carotenoid protein Phycobilisome(s) Quenching 

Abbreviations

APC

Allophycocyanin

EET

Electronic energy transfer

hECN

3′-hydroxyechinenone

ICT

Internal charge transfer

FRP

Fluorescence recovery protein

LCM

Long-wavelength terminal emitter

NPQ

Non-photochemical fluorescence quenching

OCP

Orange carotenoid protein

PBLCM

Phycobilin-containing domain of LCM

PBS

Phycobilisome

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Igor N. Stadnichuk
    • 1
  • Pavel M. Krasilnikov
    • 2
  • Dmitry V. Zlenko
    • 2
  • Alexandra Ya. Freidzon
    • 3
  • Mikhail F. Yanyushin
    • 4
  • Andrei B. Rubin
    • 2
  1. 1.K. A. Timiryazev Institute of Plant Physiology RASMoscowRussia
  2. 2.Biological Faculty of Lomonosov Moscow State UniversityMoscowRussia
  3. 3.Photochemical Centre of Russian Academy of SciencesMoscowRussia
  4. 4.Institute of Fundamental Problems of BiologyRussian Academy of SciencesPushchinoRussia

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