Photosynthesis Research

, Volume 126, Issue 2–3, pp 449–463 | Cite as

Repetitive light pulse-induced photoinhibition of photosystem I severely affects CO2 assimilation and photoprotection in wheat leaves

Regular Paper

Abstract

It was previously found that photosystem I (PSI) photoinhibition represents mostly irreversible damage with a slow recovery; however, its physiological significance has not been sufficiently characterized. The aim of the study was to assess the effect of PSI photoinhibition on photosynthesis in vivo. The inactivation of PSI was done by a series of short light saturation pulses applied by fluorimeter in darkness (every 10 s for 15 min), which led to decrease of both PSI (~60 %) and photosystem II (PSII) (~15 %) photochemical activity. No PSI recovery was observed within 2 days, whereas the PSII was fully recovered. Strongly limited PSI electron transport led to an imbalance between PSII and PSI photochemistry, with a high excitation pressure on PSII acceptor side and low oxidation of the PSI donor side. Low and delayed light-induced NPQ and P700+ rise in inactivated samples indicated a decrease in formation of transthylakoid proton gradient (ΔpH), which was confirmed also by analysis of electrochromic bandshift (ECSt) records. In parallel with photochemical parameters, the CO2 assimilation was also strongly inhibited, more in low light (~70 %) than in high light (~45 %); the decrease was not caused by stomatal closure. PSI electron transport limited the CO2 assimilation at low to moderate light intensities, but it seems not to be directly responsible for a low CO2 assimilation at high light. In this regard, the possible effects of PSI photoinhibition on the redox signaling in chloroplast and its role in downregulation of Calvin cycle activity are discussed.

Keywords

PSI photoinactivation Transthylakoid proton gradient Non-photochemical quenching Electrochromic bandshift P700 

Abbreviations

\(A_{{{\text{CO}}_{2} }}\)

CO2 assimilation rate

CET

Cyclic electron transport

cyt b6/f

Cytochrome b6/f

ECS

Electrochromic shift

ETR

Apparent electron transport rate

F0

Minimum fluorescence from dark-adapted leaf (PSII centers open)

\(F_{0}^{\prime}\)

Minimum fluorescence from light-adapted leaf

Fm, \(F_{\rm m}^{\prime}\)

Maximum fluorescence from dark- or light-adapted leaf respectively (PS II centers closed)

FNR

Ferredoxin NADP+ oxidoreductase

Fv/Fm

Maximum quantum yield of PSII photochemistry

gH+

Transthylakoid proton conductivity

LED

Light emitting diode

LHC

Light harvesting complex

NPQ

Non-photochemical quenching

P

P700 absorbance at given light intensity

P700

Primary electron donor of PSI (reduced form)

P700+

Primary electron donor of PSI (oxidized form)

PAM

Pulse-amplitude modulated

PAR

Photosynthetic active radiation

Pm, \(P_{\rm m}^{\prime}\)

Maximum P700 signal in dark- or light-adapted state

Pmf

Proton motive force

PS I

Photosystem I

PS II

Photosystem II

QA

Primary PSII acceptor

QA/QA

Total Redox poise of the primary electron acceptor of PSII (1 − qP)

qE

PH dependent energy dissipation

qL

‘Lake’ model photochemical quenching coefficient

qP

‘Puddle’ model photochemical quenching coefficient

SP

Saturation light pulse

ΔpH

Transthylakoid pH gradient

ΔpHpmf

Osmotic component of proton motive force

ΦNA

Quantum yield of non-photochemical energy dissipation in PSI due to acceptor side limitation

ΦND

Quantum yield of non-photochemical energy dissipation in PSI due to donor side limitation

ΦNO

Quantum efficiency of non-regulated energy dissipation in PSII

ΦNPQ

Quantum yield of pH-dependent energy dissipation in PSII

ΦPSI

Effective quantum yield (efficiency) of PSI photochemistry at given actinic light intensity

ΦPSII

Actual quantum yield (efficiency) of PSII photochemistry

Δψ

Transmembrane electric potential

Δψpmf

Electric component of proton motive force

Supplementary material

11120_2015_121_MOESM1_ESM.pdf (392 kb)
Supplementary material 1 (PDF 392 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Marek Zivcak
    • 1
  • Marian Brestic
    • 1
  • Kristyna Kunderlikova
    • 1
  • Oksana Sytar
    • 1
    • 2
  • Suleyman I. Allakhverdiev
    • 3
    • 4
    • 5
  1. 1.Department of Plant PhysiologySlovak Agricultural UniversityNitraSlovak Republic
  2. 2.Department of Plant Physiology and EcologyTaras Shevchenko National University of KyivKyivUkraine
  3. 3.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  4. 4.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchinoRussia
  5. 5.Department of Plant Physiology, Faculty of BiologyM.V. Lomonosov Moscow State UniversityMoscowRussia

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