Photosynthesis Research

, Volume 125, Issue 1–2, pp 151–166 | Cite as

Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines

  • Marian Brestic
  • Marek Zivcak
  • Kristyna Kunderlikova
  • Oksana Sytar
  • Hongbo Shao
  • Hazem M. Kalaji
  • Suleyman I. Allakhverdiev
Regular Paper

Abstract

In vivo analyses of electron and proton transport-related processes as well as photoprotective responses were carried out at different stages of growth in chlorophyll b (Chl b)-deficient mutant lines (ANK-32A and ANK-32B) and wild type (WT) of wheat (Triticum aestivum L.). In addition to a high Chl ab ratio, ANK mutants had a lower content of photo-oxidizable photosystem I (PSI, Pm), and several parameters indicated a low PSI/PSII ratio. Moreover, simultaneous measurements of Chl fluorescence and P700 indicated a shift of balance between redox poise of the PSII acceptor side and the PSII donor side, with preferential reduction of the plastoquinone pool, resulting in an over reduced PSI acceptor side (high ΦNA values). This was the probable reason for PSI inactivation observed in the ANK mutants, but not in WT. In later growth phases, we observed partial relief of “chlorina symptoms,” toward WT. Measurements of ΔA520 decay confirmed that, in early growth stages, the ANK mutants with low PSI content had a limited capacity to build up the transthylakoid proton gradient (ΔpH) needed to trigger non-photochemical quenching (NPQ) and to regulate the electron transport by cytochrome b6/f. Later, the increase in the PSI/PSII ratio enabled ANK mutants to reach full NPQ, but neither over reduction of the PSI acceptor side nor PSI photoinactivation due to imbalance between the activity of PSII and PSI was mitigated. Thus, our results support the crucial role of proper regulation of linear electron transport in the protection of PSI against photoinhibition. Moreover, the ANK mutants of wheat showing the dynamic developmental changes in the PSI/PSII ratio are presented here as very useful models for further studies.

Keywords

Chlorina mutants Wheat Non-photochemical quenching Chlorophyll fluorescence Transthylakoid proton gradient PSI photoinhibition 

Abbreviations

See Materials and methods section for other symbols representing chlorophyll fluorescence and P700 parameters

\(A_{{{\text{CO}}_{2} }}\)

CO2 assimilation rate

Chl

Chlorophyll

ChlF

Chlorophyll a fluorescence

Cyt b6/f

Cytochrome b6/f

ECS

Electrochromic bandshift

LED

Light emitting diode

LHC

Light harvesting complex

P700

Primary electron donor of PSI (reduced form)

P700+

Primary electron donor of PSI (oxidized form)

PAR

Photosynthetic active radiation

Pmf

Proton motive force

PQ

Plastoquinone

PSI

Photosystem I

PSII

Photosystem II

QA, QB

Primary, secondary PSII acceptor

RCs

Reaction centers

ROS

Reactive oxygen species

qE

pH-dependent energy dissipation

WT

Wild type; the genotype with normal chlorophyll synthesis

ΔA520

Absorbance changes at 520 nm

ΔpH

Transthylakoid pH gradient

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Marian Brestic
    • 1
  • Marek Zivcak
    • 1
  • Kristyna Kunderlikova
    • 1
  • Oksana Sytar
    • 1
    • 2
  • Hongbo Shao
    • 1
    • 3
  • Hazem M. Kalaji
    • 4
  • Suleyman I. Allakhverdiev
    • 5
    • 6
    • 7
  1. 1.Department of Plant PhysiologySlovak Agricultural UniversityNitraSlovak Republic
  2. 2.Department of Plant Physiology and EcologyTaras Shevchenko National University of KyivKievUkraine
  3. 3.Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC)Chinese Academy of Sciences (CAS)YantaiPeople’s Republic of China
  4. 4.Department of Plant Physiology, Faculty of Agriculture and BiologyWarsaw Agricultural University SGGWWarsawPoland
  5. 5.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  6. 6.Institute of Basic Biological ProblemsRussian Academy of SciencesMoscowRussia
  7. 7.Department of Plant Physiology, Faculty of BiologyM.V. Lomonosov Moscow State UniversityMoscowRussia

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