Photosynthesis Research

, Volume 117, Issue 1–3, pp 235–243 | Cite as

Modification of energy-transfer processes in the cyanobacterium, Arthrospira platensis, to adapt to light conditions, probed by time-resolved fluorescence spectroscopy

  • Seiji Akimoto
  • Makio Yokono
  • Shimpei Aikawa
  • Akihiko Kondo
Regular Paper
First Online:
Received:
Accepted:

Abstract

In cyanobacteria, the interactions among pigment–protein complexes are modified in response to changes in light conditions. In the present study, we analyzed excitation energy transfer from the phycobilisome and photosystem II to photosystem I in the cyanobacterium Arthrospira (Spirulina) platensis. The cells were grown under lights with different spectral profiles and under different light intensities, and the energy-transfer characteristics were evaluated using steady-state absorption, steady-state fluorescence, and picosecond time-resolved fluorescence spectroscopy techniques. The fluorescence rise and decay curves were analyzed by global analysis to obtain fluorescence decay-associated spectra. The direct energy transfer from the phycobilisome to photosystem I and energy transfer from photosystem II to photosystem I were modified depending on the light quality, light quantity, and cultivation period. However, the total amount of energy transferred to photosystem I remained constant under the different growth conditions. We discuss the differences in energy-transfer processes under different cultivation and light conditions.

Keywords

Energy transfer Light adaptation Phycobilisome Delayed fluorescence 

Abbreviations

Car

Carotenoid

Chl

Chlorophyll

FDAS

Fluorescence decay-associated spectrum (spectra)

LED

Light-emitting diodes

PBS

Phycobilisome

PS

Photosystem

TRFS

Time-resolved fluorescence spectrum (spectra)

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Notes

Acknowledgments

The authors would like to thank Mr. F. Hamada and Ms. A. Teshigahara for their help in measurements and analyses at the early stage of this study. This work was partly supported by a Grant-in-Aid for Scientific Research from JSPS (Nos. 22370017 and 23370013) to SA and a Grant-in-Aid for JSPS Fellows (No. 21A72944) to MY.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Seiji Akimoto
    • 1
    • 2
  • Makio Yokono
    • 1
    • 2
  • Shimpei Aikawa
    • 2
    • 3
  • Akihiko Kondo
    • 2
    • 3
  1. 1.Molecular Photoscience Research Center, Kobe UniversityKobeJapan
  2. 2.JST, CRESTKobeJapan
  3. 3.Graduate School of Engineering, Kobe UniversityKobeJapan

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