Photosynthesis Research

, Volume 114, Issue 3, pp 165–177 | Cite as

Wavelength-dependent photodamage to Chlorella investigated with a new type of multi-color PAM chlorophyll fluorometer

Regular Paper

Abstract

A new type of multi-color PAM chlorophyll fluorometer (Schreiber et al. 2012) was applied for measurements of photodamage to photosystem II (PS II) in optically thin suspensions of Chlorella (200 μg Chl l−1) in the presence of 1 mM lincomycin. An action spectrum of the relative decrease of Fv/Fm in the 440–625 nm range was measured, which not only showed the expected high activity in the blue, but at a lower level also substantial activity above 540 nm. With the same dilute suspension, a PS II absorption spectrum was derived via measurements of the O-I1 rise kinetics induced by differently colored strong light at defined incident quantum flux densities. After normalization of the two spectra at 625 nm, the relative extent of photodamage at 440–480 nm proved substantially higher than absorption by PS II, whereas the two spectra were close to identical in the 540–625 nm range. Hence, overall photodamage to PS II appears to consist of two components, one of which is due to light absorbed by PS II pigments, whereas the other one is likely to involve direct light absorption by Mn in the oxygen-evolving complex (Hakala et al. Biochim Biophys Acta 1706:68–80, 2005). Based on this rationale, an action spectrum of the Mn mechanism of photodamage was deconvoluted from the overall action spectrum, declining steeply above 480 nm. An almost identical Mn-spectrum was derived by another approach with the PAR of the various colors being adjusted to give identical rates of PS II turnover, PARII. The tentative, basic assumption of negligibly small contribution of the Mn mechanism to photodamage above 540 nm was supported by supplementary measurements using an external 665 nm lamp. 665 nm not only gave about two times PS II turnover as compared to 625 nm, but also about two times photodamage.

Keywords

Absorption cross-section of PS II Chlorella Chlorophyll fluorescence Mn absorption O-I1 fluorescence rise PAR Photoinhibition 

Abbreviations

AL

Actinic light

COB

Chip on board

ETR

Electron transport rate

Fm

Dark-acclimated maximal fluorescence yield

Fm′

Maximal fluorescence yield during illumination

F0

Dark-acclimated minimal fluorescence yield

FR

Far red light (>700 nm)

Fv

Variable fluorescence yield

J

Sigmoidicity/connectivity parameter

kII

Rate constant of PS II turnover

LED

Light emitting diode

ML

Pulse-modulated measuring light

NPQ

Non-photochemical quenching

OEC

Oxygen-evolving complex

O-I1

Photochemical phase of fast fluorescence rise

PAM

Pulse amplitude modulation

PAR

Photosynthetically active radiation in μmol photons m−2 s−1

PAR(λ)

PAR of a particular color of light, with λ denoting LED peak wavelength

PARII

Absolute rate of PS II quantum absorption resulting in PS II turnover characterized by rate constant kII

σII(λ)

Wavelength-dependent absorption cross-section of PS II

SP

Saturation pulse

ST

Single-turnover light pulse

τ

Time constant of light-driven QA reduction equivalent to 1/kII

Y(II)

Effective quantum yield of PS II

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Julius-von-Sachs Institut für BiowissenschaftenUniversität WürzburgWürzburgGermany

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