Photosynthesis Research

, Volume 114, Issue 3, pp 189–206 | Cite as

Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F0 fluorescence in leaves of Arabidopsis thaliana and Zea mays

  • Erhard E. Pfündel
  • Christof Klughammer
  • Armin Meister
  • Zoran G. Cerovic
Emerging Techniques

Abstract

The effect of stepwise increments of red light intensities on pulse-amplitude modulated (PAM) chlorophyll (Chl) fluorescence from leaves of A. thaliana and Z. mays was investigated. Minimum and maximum fluorescence were measured before illumination (F0 and FM, respectively) and at the end of each light step (\( F^{\prime}_{0} \) and \( F^{\prime}_{\text{M}} \), respectively). Calculated \( F^{\prime}_{0} \) values derived from F0, FM and \( F^{\prime}_{\text{M}} \) fluorescence according to Oxborough and Baker (1997) were lower than the corresponding measured \( F^{\prime}_{0} \) values. Based on the concept that calculated \( F^{\prime}_{0} \) values are under-estimated because the underlying theory ignores PSI fluorescence, a method was devised to gain relative PSI fluorescence intensities from differences between calculated and measured \( F^{\prime}_{0} \). This method yields fluorometer-specific PSI data as its input data (F0, FM, \( F^{\prime}_{0} \) and \( F^{\prime}_{\text{M}} \)) depend solely on the spectral properties of the fluorometer used. Under the present conditions, the PSI contribution to F0 fluorescence was 0.24 in A. thaliana and it was independent on the light acclimation status; the corresponding value was 0.50 in Z. mays. Correction for PSI fluorescence affected Z. mays most: the linear relationship between PSI and PSII photochemical yields was clearly shifted toward the one-to-one proportionality line and maximum electron transport was increased by 50 %. Further, correction for PSI fluorescence increased the PSII reaction center-specific parameter, 1/F0 − 1/FM, up to 50 % in A. thaliana and up to 400 % in Z. mays.

Keywords

C4 photosynthesis Light-response curve PAM fluorescence Photosynthesis Quantum yield for photochemistry 

Abbreviations

ϕPSII

Effective yield for PSII photochemistry

Chl a

Chlorophyll a

Chl b

Chlorophyll b

ETR

Electron transport rate

F0

Minimum fluorescence intensity in the dark-acclimated state

\( F^{\prime}_{0} \)

Minimum fluorescence intensity in the light-acclimated state

F1

Photosystem I fluorescence intensity

FM

Maximum fluorescence intensity in the dark-acclimated state

\( F^{\prime}_{\text{M}} \)

Maximum fluorescence intensity in the light-acclimated state

fRC2

PSII reaction center-specific factor

FV/FM

Maximum yield for primary photochemistry of PSII

GH

Green house-grown

HL

High-light acclimated

LL

Low-light grown

PPFD

Photosynthetic photon flux density

PSI

Photosystem I

PSII

Photosystem II

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Erhard E. Pfündel
    • 1
    • 2
  • Christof Klughammer
    • 3
  • Armin Meister
    • 4
  • Zoran G. Cerovic
    • 5
  1. 1.Lehrstuhl für Botanik II der Universität WürzburgJulius-von-Sachs Institut für BiowissenschaftenWürzburgGermany
  2. 2.Heinz Walz GmbHEffeltrichGermany
  3. 3.RheinfeldenGermany
  4. 4.Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)GaterslebenGermany
  5. 5.Equipe de Biospectroscopie Végétale, Laboratoire d’Ecologie Systématique et Evolution (UMR 8079) CNRS, Département d’Ecophysiologie VégétaleUniversité Paris-Sud 11Orsay CedexFrance

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