Photosynthesis Research

, Volume 114, Issue 2, pp 121–131 | Cite as

The UV-B photoreceptor UVR8 promotes photosynthetic efficiency in Arabidopsis thaliana exposed to elevated levels of UV-B

  • Matthew P. Davey
  • Novita I. Susanti
  • Jason J. Wargent
  • Jane E. Findlay
  • W. Paul Quick
  • Nigel D. Paul
  • Gareth I. JenkinsEmail author
Regular Paper


The UV-B photoreceptor UVR8 regulates expression of genes in response to UV-B, some encoding chloroplast proteins, but the importance of UVR8 in maintaining photosynthetic competence is unknown. The maximum quantum yield of PSII (F v/F m) and the operating efficiency of PSII (Φ PSII) were measured in wild-type and uvr8 mutant Arabidopsis thaliana. The importance of specific UVR8-regulated genes in maintaining photosynthetic competence was examined using mutants. Both F v/F m and Φ PSII decreased when plants were exposed to elevated UV-B, in general more so in uvr8 mutant plants than wild-type. UV-B increased the level of psbD-BLRP (blue light responsive promoter) transcripts, encoding the PSII D2 protein. This increase was mediated by the UVR8-regulated chloroplast RNA polymerase sigma factor SIG5, but SIG5 was not required to maintain photosynthetic efficiency at elevated UV-B. Levels of the D1 protein of PSII decreased markedly when plants were exposed to elevated UV-B, but there was no significant difference between wild-type and uvr8 under conditions where the mutant showed increased photoinhibition. The results show that UVR8 promotes photosynthetic efficiency at elevated levels of UV-B. Loss of the DI polypeptide is probably important in causing photoinhibition, but does not entirely explain the reduced photosynthetic efficiency of the uvr8 mutant compared to wild-type.


Arabidopsis ELIP PSII SIG5 UV-B UVR8 



G.I.J thanks the UK Biotechnology and Biological Sciences Research Council, The Leverhulme Trust and the University of Glasgow for supporting his research on UVR8. N.S. was supported by Satya Wacana Christian University, Indonesia and The Ministry of Education of Indonesia. J.J.W and N.D.P thank the UK Agricultural and Horticultural Development Board for supporting their research with a studentship to J.J.W. We are grateful to Drs Takashi Shina, Kan Tanaka and Carlo Soave for providing seeds of mutants and to Dr Bobby Brown for valuable comments on the RT-PCR experiments.


  1. Booij-James IS, Dube SK, Jansen MAK, Edelman M, Mattoo AK (2000) Ultraviolet-B radiation impacts light-mediated turnover of the photosystem II reaction center heterodimer in Arabidopsis mutants altered in phenolic metabolism. Plant Physiol 124:1275–1283PubMedCrossRefGoogle Scholar
  2. Brosché N, Strid A (2003) Molecular events following perception of ultraviolet-B radiation by plants. Physiologia Plant 117:1–10CrossRefGoogle Scholar
  3. Brown BA, Jenkins GI (2008) UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH. Plant Physiol 146:576–588PubMedCrossRefGoogle Scholar
  4. Brown BA, Cloix C, Jiang GH, Kaiserli E, Herzyk P, Kliebenstein DJ, Jenkins GI (2005) A UV-B-specific signaling component orchestrates plant UV protection. Proc Natl Acad Sci USA 102:18225–18230PubMedCrossRefGoogle Scholar
  5. Brown BA, Headland LR, Jenkins GI (2009) UV-B action spectrum for UVR8-mediated HY5 transcript accumulation in Arabidopsis. Photochem Photobiol 85:1147–1155PubMedCrossRefGoogle Scholar
  6. Caldwell MM, Bornman JF, Ballaré CL, Flint SD, Kulandaivelu G (2007) Terrestrial ecosystems, increased solar ultraviolet radiation, and interactions with other climate change factors. Photochem Photobiol Sci 6:252–266PubMedCrossRefGoogle Scholar
  7. Casati P, Walbot V (2004) Rapid transcriptome responses of maize (Zea mays) to UV-B in irradiated and shielded tissues. Genome Biol 5:R16PubMedCrossRefGoogle Scholar
  8. Christie JM, Arvai AS, Baxter KJ, Heilmann M, Pratt AJ, O’Hara A, Kelly SM, Hothorn M, Smith BO, Hitomi K, Jenkins GI, Getzoff ED (2012) Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges. Science 335:1492–1496PubMedCrossRefGoogle Scholar
  9. Cloix C, Jenkins GI (2008) Interaction of the Arabidopsis UV-B-specific signalling component UVR8 with chromatin. Mol Plant 1:118–128PubMedCrossRefGoogle Scholar
  10. Edelman M, Mattoo AK (2008) D1-protein dynamics in photosystem II: the lingering enigma. Photosynth Res 98:609–620PubMedCrossRefGoogle Scholar
  11. Favory JJ, Stec A, Gruber H, Rizzini L, Oravecz A, Funk M, Albert A, Cloix C, Jenkins GI, Oakeley EJ, Seidlitz HK, Nagy F, Ulm R (2009) Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. EMBO J 28:591–601PubMedCrossRefGoogle Scholar
  12. Foggo A, Higgins S, Wargent JJ, Coleman RA (2007) Tri-trophic consequences of UV-B exposure: plants, herbivores and parasitoids. Oecologia 154:505–512PubMedCrossRefGoogle Scholar
  13. Frohnmeyer H, Staiger D (2003) Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133:1420–1428PubMedCrossRefGoogle Scholar
  14. Greenberg BM, Gaba V, Canaani O, Malkin S, Mattoo AK, Edelman M (1989) Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction centre protein in the visible and UV spectral regions. Proc Natl Acad Sci USA 86:6617–6620PubMedCrossRefGoogle Scholar
  15. Jansen MAK, Gaba V, Greenberg BM, Mattoo AK, Edelman M (1996) Low threshold levels of UV-B in a background of photosynthetically acive radiation trigger rapid degradation of the D2 protein of photosystemn-II. Plant J 9:693–699CrossRefGoogle Scholar
  16. Jansen MAK, Gaba V, Greenberg BM (1998) Higher plants and UV-B radiation: balancing damage, repair and acclimation. Trends Plant Sci 3:131–135CrossRefGoogle Scholar
  17. Jenkins GI (2009) Signal transduction in responses to UV-B radiation. Annu Rev Plant Biol 60:407–431PubMedCrossRefGoogle Scholar
  18. Jenkins GI, Long JC, Wade HK, Shenton MR, Bibikova TN (2001) UV and blue light signalling: pathways regulating chalcone synthase gene expression in Arabidopsis. New Phytol 151:121–131CrossRefGoogle Scholar
  19. Jordan BR (1996) The effects of ultraviolet-B radiation on plants: a molecular perspective. Adv Bot Res 22:97–162CrossRefGoogle Scholar
  20. Kaiserli E, Jenkins GI (2007) UV-B promotes rapid nuclear translocation of the Arabidopsis UV-B-specific signaling component UVR8 and activates its function in the nucleus. Plant Cell 19:2662–2673PubMedCrossRefGoogle Scholar
  21. Kanamaru K, Tanaka K (2004) Roles of chloroplast RNA polymerase sigma factors in chloroplast development and stress response in higher plants. Biosci Biotechnol Biochem 68:2215–2223PubMedCrossRefGoogle Scholar
  22. Kliebenstein DJ, Lim JE, Landry LG, Last RL (2002) Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human Regulator of Chromatin Condensation 1. Plant Physiol 130:234–243PubMedCrossRefGoogle Scholar
  23. Lake JA, Field KJ, Davey MP, Beerling DJ, Lomax BH (2009) Metabolomic and physiological responses reveal multi-phasic acclimation of Arabidopsis to chronic UV radiation. Plant Cell Environm 32:1377–1389CrossRefGoogle Scholar
  24. Lerbs-Mache S (2011) Function of plastid sigma factors in higher plants: regulation of gene expression or just preservation of constitutive transcription? Plant Mol Biol 76:235–249PubMedCrossRefGoogle Scholar
  25. Mochizuki T, Onda Y, Fujiwara E, Wada M, Toyoshima Y (2004) Two independent light signals cooperate in the activation of the plastid psbD blue light-responsive promoter in Arabidopsis. FEBS Letts 571:26–30CrossRefGoogle Scholar
  26. Nagashima A, Hanaoka M, Shikanai T, Fujiwara M, Kanamaru K, Takahashi H, Tanaka K (2004) The multiple-stress responsive plastid sigma factor, SIG5, directs activation of the psbD blue light-responsive promoter (BLRP) in Arabidopsis thaliana. Plant Cell Physiol 45:357–368PubMedCrossRefGoogle Scholar
  27. Nixon P, Michoux F, Yu J, Boehm M, Komenda J (2010) Recent advances in understanding the assembly and repair of photosysten II. Ann Bot 106:1–16PubMedCrossRefGoogle Scholar
  28. Oravecz A, Baumann A, Mate Z, Brzezinska A, Molinier J, Oakeley EJ, Adam E, Schäfer E, Nagy F, Ulm R (2006) Constitutively photomorphogenic1 is required for the UV-B response in Arabidopsis. Plant Cell 18:1975–1990PubMedCrossRefGoogle Scholar
  29. Rizzini L, Favory J–J, Cloix C, Faggionato D, O’Hara A, Kaiserli E, Baumeister R, Schäfer E, Nagy F, Jenkins GI, Ulm R (2011) Perception of UV-B by the Arabidopsis UVR8 protein. Science 332:103–106PubMedCrossRefGoogle Scholar
  30. Rossini S, Casazza AP, Engelmann ECM, Havaux M, Jennings RC, Soave C (2006) Suppression of both ELIP1 and ELIP2 in Arabidopsis does not affect tolerance to photoinhibition and photooxidative stress. Plant Physiol 141:1264–1273PubMedCrossRefGoogle Scholar
  31. Rozema J, van de Staaij J, Björn LO, Caldwell M (1997) UV-B as an environmental factor in plant life: Stress and regulation. Trends Ecol Evol 12:22–28PubMedCrossRefGoogle Scholar
  32. Stracke R, Favory J–J, Gruber H, Bartelniewoehner L, Bartels S, Binkert M, Funk M, Weisshaar B, Ulm R (2010) The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG/MYB12 gene in response to light and ultraviolet-B radiation. Plant Cell Environ 33:88–103PubMedGoogle Scholar
  33. Takahashi S, Badger MR (2010) Photoprotection in plants: a new light on photosystem II damage. Trends Plant Sci 16:53–60PubMedCrossRefGoogle Scholar
  34. Takahashi S, Murata N (2008) How do environmental stresses accelerate photoinhibition? Trends Plant Sci 13:178–182PubMedCrossRefGoogle Scholar
  35. Takahashi S, Milward SE, Yamori W, Evans RJ, Hillier W, Badger MR (2010) The solar action spectrum of photosystem II damage. Plant Physiol 153:988–993PubMedCrossRefGoogle Scholar
  36. Teramura AH, Sullivan JH (1994) Effects of UV-B radiation on photosynthesis and growth of terrestrial plants. Photosynth Res 39:463–473CrossRefGoogle Scholar
  37. Tsunoyama Y, Ishizaki Y, Morikawa K, Kobori M, Nakahira Y, Takeba G, Toshinori Y, Shiina T (2004) Blue light-induced transcription of plastid-encoded psbD gene is mediated by a nuclear-encoded transcription initiation factor, AtSig5. Proc Natl Acad Sci USA 101:3304–3309PubMedCrossRefGoogle Scholar
  38. Ulm R, Nagy F (2005) Signalling and gene regulation in response to ultraviolet light. Curr Op Plant Biol 8:477–482CrossRefGoogle Scholar
  39. Ulm R, Baumann A, Oravecz A, Mate Z, Adam E, Oakeley EJ, Schäfer E, Nagy F (2004) Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis. Proc Natl Acad Sci USA 101:1397–1402PubMedCrossRefGoogle Scholar
  40. Wargent JJ, Gegas VC, Jenkins GI, Doonan JH, Paul ND (2009) UVR8 in Arabidopsis thaliana regulates multiple aspects of cellular differentiation during leaf development in response to ultraviolet B radiation. New Phytol 183:315–326PubMedCrossRefGoogle Scholar
  41. Wargent JJ, Elfadly EM, Moore JP, Paul ND (2011) Increased exposure to UV-B radiation during early development leads to enhanced photoprotection and improved long-term performance in Lactuca sativa. Plant Cell Environm 34:1401–1413CrossRefGoogle Scholar
  42. Wormuth D, Baier M, Kandlbinder A, Scheibe R, Hartung RW, Dietz K-J (2006) Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability. BMC Plant Biol 6:15PubMedCrossRefGoogle Scholar
  43. Wu D, Hu Q, Yan Z, Chen W, Yan C, Huang X, Zhang J, Yang P, Deng H, Wang J, Deng X, Shi Y (2012) Structural basis of ultraviolet-B perception by UVR8. Nature 484:214–219PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Matthew P. Davey
    • 2
    • 4
  • Novita I. Susanti
    • 1
    • 5
  • Jason J. Wargent
    • 3
    • 6
  • Jane E. Findlay
    • 1
  • W. Paul Quick
    • 2
  • Nigel D. Paul
    • 3
  • Gareth I. Jenkins
    • 1
    Email author
  1. 1.Institute of Molecular Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of GlasgowGlasgowUK
  2. 2.Department of Animal and Plant SciencesUniversity of SheffieldSheffieldUK
  3. 3.Lancaster Environment Centre, Lancaster UniversityLancasterUK
  4. 4.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  5. 5.Department of Wine Food and Molecular BioscienceFaculty of Agriculture and Life Science, Lincoln UniversityChristchurchNew Zealand
  6. 6.Institute of Natural Resources, Massey UniversityPalmerston NorthNew Zealand

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