An alphaproteobacterium capable of both aerobic and anaerobic anoxygenic photosynthesis but incapable of photoautotrophy: Charonomicrobium ambiphototrophicum, gen. nov., sp. nov.
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A facultatively aerobic deep brown coccoid to ovoid bacterium, strain EG17T, was isolated from a saline effluent stream in the NaCl-dominated brine spring system known as East German Creek in the province of Manitoba, Canada. The strain produced BChl a incorporated into a functional reaction center and two light-harvesting complexes with absorption peaks at 802, 850, and 879 nm. EG17T is the first reported anoxygenic phototroph capable of photoheterotrophic growth under both oxic and anoxic conditions. It yielded proportionally the greatest aerobic photosynthetic biomass under oligotrophic conditions. The results of 16S rRNA gene sequence comparisons revealed that EG17T was related most closely to the aerobic anoxygenic phototrophs Roseibacterium elongatum (98.3%) and quite distantly to both Dinoroseobacter shibae (95.2%) and Roseicyclus mahoneyensis (94.7%). The DNA G + C content was 65.6 mol%. On the basis of the unique dual aerobic/anaerobic photosynthetic capability, the distinctive spectrophotometric absorption of the photosynthetic apparatus, diagnostic physiological and biochemical traits, and the moderate phylogenetic separation between EG17T and its nearest relatives, it is concluded that this microorganism should be classified as a novel genus and species, Charonomicrobium ambiphototrophicum gen. nov., sp. nov., with EG17T as the type strain.
KeywordsCharonomicrobium ambiphototrophicum AAP Aerobic anoxygenic phototorphs Anoxygenic photosynthesis Purple nonsulfur bacteria Photoautotrophy Bacteriochlorophyll a
This research was supported by a grant from the Natural Science and Engineering Research Council (NSERC, Canada) to V. Yurkov.
- Felsenstein J (1993) PHYLIP (phylogenetic inference package) version 3.5.1. Department of Genetics. University of Washington, SeattleGoogle Scholar
- Gosink JJ, Herwig RP, Staley JT (1997) Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolated bacteria from polar sea ice and water. Syst Appl Microbiol 20:356–365Google Scholar
- Grasby SE (2000) Saline spring geochemistry, west-central Manitoba. In: Report of activities 2000. Manitoba Industry, Trade and Mines, Manitoba Geological Survey, Winnipeg, pp 214–216Google Scholar
- McKillop WB, Patterson RT, Delorme LD, Nogrady T (1992) The origin, physico-chemistry and biotics of sodium chloride dominated saline waters on the western shore of Lake Winnipegosis, Manitoba. Can Field-Nat 106:454–473Google Scholar
- Sorokin DY, Tourova TP, Kuznetsov BB, Bryantseva IA, Gorlenko VM (2000) Roseinatronobacter thiooxidans gen. nov., sp. nov., a new alkaliphilic aerobic bacteriochlorophyll a-containing bacterium isolated from a soda lake. Mikrobiologiya 69:75–82Google Scholar
- Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfor P, Maiden MCJ, Nesme X, Roselló-Mora R, Swings J, Trüper HG, Vauterin L, Ward AC, Whitman WB (2002) Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047PubMedCrossRefGoogle Scholar
- Swingley WD, Sadekar S, Mastrian SD, Matthies HJ, Hao J, Ramos H, Acharya CR, Conrad AL, Taylor HL, Dejesa LC, Shah MK, O’Huallachain ME, Lince MT, Blankenship RE, Beatty JT, Touchman JW (2007) The complete genome sequence of Roseobacter denitrificans reveals a mixotrophic as opposed to photosynthetic metabolism. J Bacteriol 189:683–690PubMedCrossRefGoogle Scholar
- van Dorssen RJ, Hunter CN, van Grondelle R, Korenhof AH, Amesz J (1987) Spectroscopic properties of antenna complexes of Rhodobacter sphaeroides in vivo. Biochim et Biophys Acta 932:179–188Google Scholar
- Yurkov VV, Csotonyi JT (2003) Aerobic anoxygenic phototrophs and heavy metalloid reducers from extreme environments. In: Pandalai SG (ed) Recent research developments in bacteriology, vol 1. Transworld Research Network, Trivandrum, pp 247–300Google Scholar
- Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P, Golecki J, Gad’on N, Gorlenko VM, Kompantseva EI, Drews G (1994) Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–434PubMedCrossRefGoogle Scholar