Photosynthesis Research

, Volume 104, Issue 2–3, pp 211–219 | Cite as

The lamellar spacing in self-assembling bacteriochlorophyll aggregates is proportional to the length of the esterifying alcohol

  • Jakub Pšenčík
  • Mika Torkkeli
  • Anita Zupčanová
  • František Vácha
  • Ritva E. Serimaa
  • Roman Tuma
Regular Paper

Abstract

Chlorosomes from green photosynthetic bacteria are large photosynthetic antennae containing self-assembling aggregates of bacteriochlorophyll c, d, or e. The pigments within chlorosomes are organized in curved lamellar structures. Aggregates with similar optical properties can be prepared in vitro, both in polar as well as non-polar solvents. In order to gain insight into their structure we examined hexane-induced aggregates of purified bacteriochlorophyll c by X-ray scattering. The bacteriochlorophyll c aggregates exhibit scattering features that are virtually identical to those of native chlorosomes demonstrating that the self-assembly of these pigments is fully encoded in their chemical structure. Thus, the hexane-induced aggregates constitute an excellent model to study the effects of chemical structure on assembly. Using bacteriochlorophyllides transesterified with different alcohols we have established a linear relationship between the esterifying alcohol length and the lamellar spacing. The results provide a structural basis for lamellar spacing variability observed for native chlorosomes from different species. A plausible physiological role of this variability is discussed. The X-ray scattering also confirmed the assignments of peaks, which arise from the crystalline baseplate in the native chlorosomes.

Keywords

Green photosynthetic bacteria Chlorosome Bacteriochlorophyll Aggregate Bacteriochlorophyllide X-ray scattering 

Notes

Acknowledgments

This study was supported by Czech Ministry of Education, Youth and Sports (projects MSM0021620835, MSM6007665808, AV0Z50510513) and Czech Science Foundation (206/09/0375); R.T. was supported by Academy of Finland (project 118462) and The University of Leeds.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jakub Pšenčík
    • 1
    • 2
  • Mika Torkkeli
    • 3
  • Anita Zupčanová
    • 2
    • 4
  • František Vácha
    • 2
    • 4
  • Ritva E. Serimaa
    • 3
  • Roman Tuma
    • 5
  1. 1.Department of Chemical Physics and Optics, Faculty of Mathematics and PhysicsCharles UniversityPragueCzech Republic
  2. 2.Institute of Physical BiologyUniversity of South BohemiaNové HradyCzech Republic
  3. 3.Department of PhysicsUniversity of HelsinkiHelsinkiFinland
  4. 4.Biology CentreAcademy of Sciences of the Czech RepublicČeské BudějoviceCzech Republic
  5. 5.The Astbury Centre for Structural Molecular BiologyUniversity of LeedsLeedsUK

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