Photosynthesis Research

, 102:95 | Cite as

Modification of a French pressure cell to improve microbial cell disruption

Technical Communication

Abstract

A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells.

Keywords

French press Bacterial lysis Photosynthetic bacteria 

References

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  3. Vanderheiden GJ, Fairchild AC, Jago AC (1970) Construction of a laboratory press for use with the French pressure cell. Appl Microbiol 19:875–877PubMedGoogle Scholar
  4. Walters JR, Stahly DP (1968) Modification of the valve of the French pressure cell. Appl Microbiol 16:1605PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Paul R. Jaschke
    • 1
  • Ian Drake
    • 2
  • J. Thomas Beatty
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of British ColumbiaVancouverCanada
  2. 2.The Life Sciences Centre—Equipment Services WorkshopUniversity of British ColumbiaVancouverCanada

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