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Photosynthesis Research

, Volume 102, Issue 1, pp 67–76 | Cite as

Probing of photosynthetic reactions in four phytoplanktonic algae with a PEA fluorometer

  • T. K. Antal
  • D. N. Matorin
  • L. V. Ilyash
  • A. A. Volgusheva
  • V. Osipov
  • I. V. Konyuhov
  • T. E. Krendeleva
  • A. B. Rubin
Regular Paper

Abstract

High-resolution light-induced kinetics of chlorophyll fluorescence (OJIP transients) were recorded and analyzed in cultures of diatoms (Thalassiosira weissflogii, Chaetoceros mulleri) and dinoflagellates (Amphidinium carterae, Prorocentrum minimum). Fluorescence transients showed the rapid exponential initial rise from the point O indicating low connectivity between PS II units and high absorption cross-section of PS II antenna. Dark-adapted dinoflagellates revealed capability to maintain the PS I-mediated re-oxidation of the PQ pool at the exposure to strong actinic light that may lead to the underestimation of F M value. In OJIP transients recorded in phytoplanktonic algae the fluorescence yield at the point O exceeded F O level because QA has been already partly reduced at 50 μs after the illumination onset. PEA was also employed to study the recovery of photosynthetic reactions in T. weissflogii during incubation of nitrogen starved cells in N-replete medium. N limitation caused the impairment of electron transport between QA and PQs, accumulation of closed PS II centers, and the reduced ability to generate transmembrane ΔpH upon illumination, almost fully restored during the recovery period. The recovered cells showed much higher values of NPQ than control ones suggesting maximization of photoprotection mechanisms in the population with a ‘stress history.’

Keywords

Chlorophyll a fluorescence OJIP transients Phytoplankton Nitrogen starvation 

Abbreviations

Chl

Chlorophyll

PS

Photosystem

P680

The primary electron donor in PS II

QA

The primary quinone electron acceptor in PS II

QB

The secondary quinone electron acceptor in PS II

PQ

Plastoquinone

OEC

Oxygen evolving complex

FNR

Ferredoxin-NADP reductase

DCMU

3-(3,4-Dichlorophenyl)-1,1-dimethylurea

N

Nitrogen

NPQ

Non-photochemical Chl fluorescence quenching

PPFD

Photosynthetic photon flux density

O, J, D, I, P

Particular steps in light-induced Chl fluorescence curve

PAM

Pulse amplitude modulated fluorometer

PEA

Plant efficiency analyzer

Notes

Acknowledgment

This work was supported by the Russian Foundation of Basic Research (07-04-00222) and by the International Science and Technology Center (3410).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • T. K. Antal
    • 1
  • D. N. Matorin
    • 1
  • L. V. Ilyash
    • 1
  • A. A. Volgusheva
    • 1
  • V. Osipov
    • 1
  • I. V. Konyuhov
    • 1
  • T. E. Krendeleva
    • 1
  • A. B. Rubin
    • 1
  1. 1.Faculty of BiologyMoscow State UniversityMoscowRussia

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