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Photosynthesis Research

, Volume 102, Issue 2–3, pp 377–390 | Cite as

Spin labeling EPR

  • Johann P. Klare
  • Heinz-Jürgen SteinhoffEmail author
Review

Abstract

Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin–transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.

Keywords

DEER Nitroxide PELDOR Polarity Photosynthetic reaction center Sensory rhodopsin Spin label accessibility Spin label mobility 

Abbreviations

AS

Amphipathic sequence

CrOx

Chromium oxalate

cw

Continuous wave

DEER

Double electron–electron resonance

EPR

Electron paramagnetic resonance

MTSSL

(1-Oxyl-2,2,5,5-tetramethylpyrroline-3-methyl)methanethiosulfonate spin label

NpSRII

Natronomonas pharaonis sensory rhodopsin II

NpHtrII

Natronomonas pharaonis halobacterial transducer II

PELDOR

Pulse electron–electron double resonance

PML

Purple membrane lipids

RC

Reaction center

SDSL

Site-directed spin labeling

TM

Transmembrane helix

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Physics DepartmentUniversity of OsnabrückOsnabrückGermany

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