Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp. ORS 278

  • Marianne Jaubert
  • Laure Hannibal
  • Joël Fardoux
  • Eric Giraud
  • André Verméglio
Regular Paper
First Online:
Received:
Accepted:

Abstract

In aerobic anoxygenic phototrophs, oxygen is required for both the formation of the photosynthetic apparatus and an efficient cyclic electron transfer. Mutants of Bradyrhizobium sp. ORS278 affected in photosystem synthesis were selected by a bacteriochlorophyll fluorescence-based screening. Out of the 9,600 mutants of a random Tn5 insertion library, 50 clones, corresponding to insertions in 28 different genes, present a difference in fluorescence intensity compared to the WT. Besides enzymes and regulators known to be involved in photosystem synthesis, 14 novel components of the photosynthesis control are identified. Among them, two genes, hsIU and hsIV, encode components of a protein degradation complex, probably linked to the renewal of photosystem, an important issue in Bradyrhizobia which have to deal with harmful reactive oxygen species. The presence of homologs in non-photosynthetic bacteria for most of the regulatory genes identified during study suggests that they could be global regulators, as the RegA–RegB system.

Keywords

Photosystem regulation Aerobic photosynthetic bacteria Bradyrhizobium 

Abbreviation

AAP

Aerobic anoxygenic phototroph

Bchl

Bacteriochlorophyll

CDS

Coding sequense

LH

Light-harvesting

PHA

Polyhydroxyalkanoates

RC

Photochemical reaction center

Rb.

Rhodobacter

Rps.

Rhodopseudomonas

RR

Response regulator

SD

Standard deviation

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Notes

Acknowledgments

M. J. is indebted to the “Ministère de l’Education Nationale, de l’Enseignement Supérieur et de la Recherche” for a doctoral grant.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Marianne Jaubert
    • 1
  • Laure Hannibal
    • 1
  • Joël Fardoux
    • 1
  • Eric Giraud
    • 1
  • André Verméglio
    • 2
    • 3
    • 4
  1. 1.Laboratoire des Symbioses Tropicales et MéditerranéennesIRD, CIRAD, AGRO-M, INRA, UM2. TA A-82/J, Campus de BaillarguetMontpellier Cedex 5France
  2. 2.CEA, DSV, IBEB, Lab Bioenerget CellulaireSaint-Paul-lez-DuranceFrance
  3. 3.CNRS, UMR Biol Veget & Microbiol EnvironSaint-Paul-lez-DuranceFrance
  4. 4.Aix-Marseille UniversitéSaint-Paul-lez-DuranceFrance

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