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Photosynthesis Research

, Volume 100, Issue 1, pp 7–17 | Cite as

Phycobiliprotein diffusion in chloroplasts of cryptophyte Rhodomonas CS24

  • Tihana Mirkovic
  • Krystyna E. Wilk
  • Paul M. G. Curmi
  • Gregory D. Scholes
Regular Paper

Abstract

Unicellular cryptophyte algae employ antenna proteins with phycobilin chromophores in their photosynthetic machinery. The mechanism of light harvesting in these organisms is significantly different than the energy funneling processes in phycobilisomes utilized by cyanobacteria and red algae. One of the most striking features of cryptophytes is the location of the water-soluble phycobiliproteins, which are contained within the intrathylakoid spaces and are not on the stromal side of the lamellae as in the red algae and cyanobacteria. Studies of mobility of phycobiliproteins at the lumenal side of the thylakoid membranes and how their diffusional behavior may influence the energy funneling steps in light harvesting are reported. Confocal microscopy and fluorescence recovery after photobleaching (FRAP) are used to measure the diffusion coefficient of phycoerythrin 545 (PE545), the primary light harvesting protein of Rhodomonas CS24, in vivo. It is concluded that the diffusion of PE545 in the lumen is inhibited, suggesting possible membrane association or aggregation as a potential source of mobility hindrance.

Keywords

Cryptophyte Phycobiliprotein Diffusion Fluorescence recovery after photobleaching Thylakoid membrane Energy transfer 

Abbreviations

PE545

Phycoerythrin 545

FRAP

Fluorescence recovery after photobleaching

PC

Phycocyanin

PS I

Photosystem I

PSII

Photosystem II

LHC

Light harvesting complex

GFP

Green fluorescent protein

TEM

Transmission electron microscopy

PEB

Phycoerythrobilin

DBV

15, 16-dihydrobiliverdin

OD

Optical density

Notes

Acknowledgments

The Natural Sciences and Engineering Research Council of Canada are gratefully acknowledged for support of this research. G.D.S. acknowledges the support of an E.W.R. Steacie Memorial Fellowship. This research is supported by grants from the Australian Research Council.

Supplementary material

11120_2009_9412_MOESM1_ESM.pdf (357 kb)
Supplementary material 1 (PDF 357 kb)

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Tihana Mirkovic
    • 1
  • Krystyna E. Wilk
    • 2
  • Paul M. G. Curmi
    • 2
  • Gregory D. Scholes
    • 1
  1. 1.Department of Chemistry, Institute for Optical Sciences, Centre for Quantum Information and Quantum ControlUniversity of TorontoTorontoCanada
  2. 2.School of Physics and Centre for ImmunologyThe University of New South WalesSydneyAustralia

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