Photosynthesis Research

, Volume 99, Issue 3, pp 185–193 | Cite as

Effect of monogalactosyldiacylglycerol on the interaction between photosystem II core complex and its antenna complexes in liposomes of thylakoid lipids

  • Feng Zhou
  • Shuang Liu
  • Zhaohui Hu
  • Tingyun Kuang
  • Harald Paulsen
  • Chunhong Yang
Regular Paper
First Online:
Received:
Accepted:

Abstract

The non-bilayer lipid monogalactosyldiacylglycerol (MGDG) is the most abundant type of lipid in the thylakoid membrane and plays an important role in regulating the structure and function of photosynthetic membrane proteins. In this study, we have reconstituted the isolated major light-harvesting complexes of photosystem II (PSII) (LHCIIb) and a preparation consisting of PSII core complexes and minor LHCII of PSII (PSIICC) into liposomes that consisted of digalactosyldiacylglycerol (DGDG), sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG), with or without MGDG. Transmission electron microscopy and freeze-fracture studies showed unilamellar proteoliposomes, and demonstrated that most of the MGDG is incorporated into bilayer structures. The impact of MGDG on the functional interaction between LHCIIb and PSIICC was investigated by low temperature (77 K) fluorescence emission spectra and the photochemical activity of PSII. The additional incorporation of LHCIIb into liposomes containing PSIICC markedly increased oxygen evolution of PSIICC. Excitation at 480 nm of chlorophyll (Chl) b in LHCIIb stimulated a characteristic fluorescence emission of the Chl a in PSII (684.2 nm), rather than that of the Chl a in LHCIIb (680 nm) in the LHCIIb–PSIICC proteoliposomes, which indicated that the energy was transferred from LHCIIb to PSIICC in liposome membranes. Increasing the percentage of MGDG in the PSIICC–LHCIIb proteoliposomes enhanced the photochemical activity of PSII, due to a more efficient energy transfer from LHCIIb to PSIICC and, thus, an enlarged antenna cross section of PSII.

Keywords

Photosystem II core complex Light-harvesting complex II Monogalactosyldiacylglycerol Liposome Photochemical activity 

Abbreviations

BBY

PSII-enriched thylakoid membranes

Chl

Chlorophyll

DCIP

2,6-dichlorophenol indophenol

DGDG

Digalactosyldiacylglycerol

DPC

1,5-diphenylcarbazide

EM

Electron micrograph

LHCII

The light-harvesting complexes of photosystem II with apoproteins Lhcb1-6

LHCIIb

Major light-harvesting complexes of photosystem II with apoproteins Lhcb1-3

MGDG

Monogalactosyldiacylglycerol

PC

Phosphatidylcholine

PG

Phosphatidylglycerol

PS

Photosystem

PSIICC

Photosystem II preparation containing core complexes, minor LHCII and part of LHCIIb

SQDG

Sulfoquinovosyldiacylglycerol

TLC

Thin layer chromatography

Triton X-100

Octylphenolpoly (ethyleneglycolether)10

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Notes

Acknowledgements

This work was supported by the National Sciences Foundation of China (No. 30670498, to C. Y. and No. 30500101, to Z. H.); the Deutsche Forschungsgemeinschaft (Pa 324/5–3, to H.P.); and the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-YW-R-137, to C.Y.).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Feng Zhou
    • 1
    • 2
  • Shuang Liu
    • 1
  • Zhaohui Hu
    • 1
  • Tingyun Kuang
    • 1
  • Harald Paulsen
    • 3
  • Chunhong Yang
    • 1
    • 3
  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of BotanyChinese Academy of SciencesBeijingChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.Institut für Allgemeine BotanikJohannes-Gutenberg-Universität MainzMainzGermany

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