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Photosynthesis Research

, Volume 95, Issue 2–3, pp 247–252 | Cite as

Relative binding affinities of chlorophylls in peridinin–chlorophyll–protein reconstituted with heterochlorophyllous mixtures

  • T. H. P. Brotosudarmo
  • S. Mackowski
  • E. Hofmann
  • R. G. Hiller
  • C. Bräuchle
  • H. ScheerEmail author
Regular Paper

Abstract

Peridinin–chlorophyll–protein (PCP), containing differently absorbing chlorophyll derivatives, are good models with which to study energy transfer among monomeric chlorophylls (Chls) by both bulk and single-molecule spectroscopy. They can be obtained by reconstituting the N-terminal domain of the protein (N-PCP) with peridinin and chlorophyll mixtures. Upon dimerization of these “half-mers”, homo- and heterochlorophyllous complexes are generated, that correspond structurally to monomeric protomers of native PCP from Amphidinium carterae. Heterochlorophyllous complexes contain two different Chls in the two halves of the complete structure. Here, we report reconstitution of N-PCP with binary mixtures of Chl a, Chl b, and [3-acetyl]-Chl a. The ratios of the pigments were varied in the reconstitution mixture, and relative binding constants were determined from quantification of these pigments in the reconstituted PCPs. We find higher affinities for both Chl b and [3-acetyl]-Chl a than for the native pigment, Chl a.

Keywords

Dinoflagellate Light-harvesting Peridinin–chlorophyll–protein Chlorophyll Binding affinity 

Abbreviations

A

Amphidinium

CD

Circular dichroism

Chl

Chlorophyll

I-PCP

Integral PCP

N-PCP

N-terminal domain of PCP

PCP

Peridinin–chlorophyll–protein

Per

Peridinin

Notes

Acknowledgments

Work was supported by the Deutsche Forschungsgemeinschaft, Bonn (SFB 533, projects A6 and B7). T.H.P.B. is indebted for financial support from Ludwig-Maximilians University, München. S.M. acknowledges a grant from the Alexander-von-Humboldt foundation, Bonn. Work was supported by Center for Integrated Protein Science Munich (CiPSM).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • T. H. P. Brotosudarmo
    • 1
    • 2
  • S. Mackowski
    • 1
  • E. Hofmann
    • 3
  • R. G. Hiller
    • 4
  • C. Bräuchle
    • 1
  • H. Scheer
    • 5
    Email author
  1. 1.Department of Chemistry and Biochemistry, Center for NanoscienceLudwig-Maximilians-UniversityMunchenGermany
  2. 2.IBLS Division of Biochemistry and Molecular Biology, Biomedical Research BuildingUniversity of GlasgowGlasgowUK
  3. 3.Department of BiologyRuhr-University BochumBochumGermany
  4. 4.School of Biological SciencesMacquarie UniversityNorth RydeAustralia
  5. 5.Department of BiologyLudwig-Maximilians-UniversityMunchenGermany

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