Photosynthesis Research

, Volume 94, Issue 2–3, pp 225–233 | Cite as

Engineering model proteins for Photosystem II function

review

Abstract

Our knowledge of Photosystem II and the molecular mechanism of oxygen production are rapidly advancing. The time is now ripe to exploit this knowledge and use it as a blueprint for the development of light-driven catalysts, ultimately for the splitting of water into O2 and H2. In this article, we outline the background and our approach to this technological application through the reverse engineering of Photosystem II into model proteins.

Keywords

Biocatalyst H2 production Photosystem II Protein engineering Water splitting 

Abbreviations

BChl

Bacteriochlorophyll

BRC

Bacterial reaction center

BFR

Bacterioferritin

Chl

Chlorophyll

CoQ0

Coenzyme Q without the hydrophobic tail

Cyt

Cytochrome

Em

Midpoint redox potential

H-bond

Hydrogen bond

LH(C)

Light harvesting (complex)

M4

Tetranuclear manganese/calcium cluster

P

Reaction center chlorophyll complex

PSII(I)

Photosystem II (I)

Q

Quinone

Rubisco

Ribulose-1,5-bisphosphate carboxylase

Y

Redox-active tyrosine

Notes

Acknowledgements

The authors thank Les Dutton and Wolfgang Lubitz for their encouragement and support throughout the development of this project. Financial assistance was provided by a grant from the Australian Research Council (ARC DP0450421).

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Tom Wydrzynski
    • 1
  • Warwick Hillier
    • 1
  • Brendon Conlan
    • 1
  1. 1.Research School of Biological SciencesAustralian National UniversityCanberraAustralia

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