Photosynthesis Research

, Volume 95, Issue 1, pp 101–109 | Cite as

Ribulose-1,5-bisphosphate carboxylase/oxygenase from thermophilic cyanobacterium Thermosynechococcus elongatus

  • Beata Gubernator
  • Rafal Bartoszewski
  • Jaroslaw Kroliczewski
  • Guenter Wildner
  • Andrzej Szczepaniak
Regular Paper

Abstract

Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) can be divided into two branches: the “red-like type” of marine algae and the “green-like type” of cyanobacteria, green algae, and higher plants. We found that the “green-like type” rubisco from the thermophilic cyanobacterium Thermosynechococcus elongatus has an almost 2-fold higher specificity factor compared with rubiscos of mesophilic cyanobacteria, reaching the values of higher plants, and simultaneously revealing an improvement in enzyme thermostability. The difference in the activation energies at the transition stages between the oxygenase and carboxylase reactions for Thermosynechococcus elongatus rubisco is very close to that of Galdieria partita and significantly higher than that of spinach. This is the first characterization of a “green-like type” rubisco from thermophilic organism.

Keywords

Rubisco Specificity factor Thermostability Thermophilic cyanobacteria 

Abbreviations

RuBP

Ribulose-1,5-bisphosphate

SF

Specificity factor

DTT

1,4-Dithiothreitol

PMSF

Phenylmethylsulfonyl fluoride

PGA

3-Phosphoglyceric acid

Notes

Acknowledgments

This work was supported by a grant from the State Committee for Scientific Research (grant no. 2 P04C 050 28) to A.S. The authors are grateful to the VW foundation for their generous support. We thank L. Wallach for technical assistance.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Beata Gubernator
    • 1
  • Rafal Bartoszewski
    • 1
  • Jaroslaw Kroliczewski
    • 1
  • Guenter Wildner
    • 2
    • 3
  • Andrzej Szczepaniak
    • 1
  1. 1.Department of BiotechnologyUniversity of WroclawWroclawPoland
  2. 2.Department of BiologyRuhr-University BochumBochumGermany
  3. 3.Department of Biochemistry and Molecular BiophysicsUniversity of ArizonaTucsonUSA

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