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Photosynthesis Research

, Volume 94, Issue 1, pp 109–120 | Cite as

Dark inactivation of ferredoxin-NADP reductase and cyclic electron flow under far-red light in sunflower leaves

  • Eero Talts
  • Vello Oja
  • Heikko Rämma
  • Bahtijor Rasulov
  • Agu Anijalg
  • Agu Laisk
Research Article

Abstract

The oxidation kinetics under far-red light (FRL) of photosystem I (PSI) high potential donors P700, plastocyanin (PC), and cytochrome f (Cyt f) were investigated in sunflower leaves with the help of a new high-sensitivity photometer at 810 nm. The slopes of the 810 nm signal were measured immediately before and after FRL was turned on or off. The same derivatives (slopes) were calculated from a mathematical model based on redox equilibrium between P700, PC and Cyt f and the parameters of the model were varied to fit the model to the measurements. Typical best-fit pool sizes were 1.0–1.5 μmol m−2 of P700, 3 PC/P700 and 1 Cyt f/P700, apparent equilibrium constants were 15 between P700 and PC and 3 between PC and Cyt f. Cyclic electron flow (CET) was calculated from the slope of the signal after FRL was turned off. CET activated as soon as electrons accumulated on the PSI acceptor side. The quantum yield of CET was close to unity. Consequently, all PSI in the leaf were able to perform in cycle, questioning the model of compartmentation of photosynthetic functions between the stroma and grana thylakoids. The induction of CET was very fast, showing that it was directly redox-controlled. After longer dark exposures CET dominated, because linear e transport was temporarily hindered by the dark inactivation of ferredoxin-NADP reductase.

Keywords

Leaf Photosystem I Cyclic electron flow Far-red light Ferredoxin-NADP reductase 

Abbreviations

CET

Cyclic electron transport

Cyt

Cytochrome

Fd

Ferredoxin

FNR

Ferredoxin-NADP reductase

FRL

Far-red light

PC

Plastocyanin

PFD, PAD

Photon flux density, incident and absorbed

PQ

Plastoquinone

PSI, PSII

Photosystem I and photosystem II

P700

Donor pigment of PSI

WL

White light

Notes

Acknowledgments

This work was funded by targeted financing theme 180517s98 from Estonian Ministry of Education and supported by grants 6607 and 6611 from Estonian Science Foundation.

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Eero Talts
    • 1
  • Vello Oja
    • 1
  • Heikko Rämma
    • 1
  • Bahtijor Rasulov
    • 1
  • Agu Anijalg
    • 2
  • Agu Laisk
    • 1
  1. 1.Institute for Molecular and Cell BiologyTartu UniversityTartuEstonia
  2. 2.Institute of PhysicsTartu UniversityTartuEstonia

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