Photosynthesis Research

, Volume 92, Issue 2, pp 181–185

From Chlorella to chloroplasts: a personal note

Original Paper


An historical and personal reflection on the function of the Benson–Calvin Cycle in isolated chloroplasts, the role of inorganic phosphate and the manner in which this might be best presented to students.


Benson–Calvin cycle Chloroplasts Phosphate 


  1. Baldry CW, Bucke C, Walker DA (1966) Incorporation of inorganic phosphate into sugar phosphates during carbon dioxide fixation by illuminated chloroplasts. Nature 210:793–796CrossRefGoogle Scholar
  2. Bandurski RS, Greiner CM (1953) The enzymatic synthesis of oxalacetate from phosphoryl-enolpyruvate and carbon dioxide. J Biol Chem 204:781–786PubMedGoogle Scholar
  3. Bassham JA, Benson AA, Kay LD, Harris AZ, Wilson AT, Calvin M (1954) The Path of carbon in photosynthesis. XXI. The cyclic regeneration of carbon dioxide acceptor. J Am Chem Soc 76:1760–1770CrossRefGoogle Scholar
  4. Benson AA, Bassham JA, Calvin M, Goodale TC, Haas VA, Stepka W (1950) The path of carbon in photosynthesis. V. paper chromatography and radioautography of the products. J Am Chem Soc 72:1710–1718CrossRefGoogle Scholar
  5. Buchanan BB (1980) Role of light in the regulation of chloroplast enzymes. Ann Rev Plant Physiol 31:341–374CrossRefGoogle Scholar
  6. Buchanan BB (1991) Regulation of CO2 assimilation in oxygenic photosynthesis: the ferredoxin/thioredoxin system. Perspective on its discovery, present status, and future development. Arch Biochem Biophys 288:1–9PubMedCrossRefGoogle Scholar
  7. Buchanan BB, Schürmann P, Wolosiuk RA, Jacquot JP (2002) The ferredoxin/thioredoxin system: from discovery to molecular structures and beyond. Photosyn Res 73:215–222PubMedCrossRefGoogle Scholar
  8. Calvin M, Benson AA (1948) The Path of Carbon in Photosynthesis. Science 107:476–480CrossRefPubMedGoogle Scholar
  9. Cockburn W, Baldry CW, Walker DA (1967a) Oxygen evolution by isolated chloroplasts with carbon dioxide as the hydrogen acceptor. A requirement for orthophosphate or pyrophosphate. Biochim Biophys Acta 131:594–596CrossRefGoogle Scholar
  10. Cockburn W, Baldry CW, Walker DA (1967b) some effects of inorganic phosphate on O2 evolution by isolated chloroplasts. Biochim Biophys Acta 143:614–624CrossRefGoogle Scholar
  11. Cockburn W, Walker DA, Baldry CW (1968) Photosynthesis by isolated chloroplasts. Reversal of orthophosphate inhibition by Calvin-cycle intermediates. Biochem J 107:89–95PubMedGoogle Scholar
  12. Chapman RE (1924) The Carbohydrate Enzymes of some starch-free monocotyledons. Biochem J 18:1388–1400PubMedGoogle Scholar
  13. Edwards GE, Walker DA (1983) C3, C4, mechanisms, and cellular and environmental regulation of photosynthesis. Blackwell Scientific Publications Ltd, Oxford, pp 1–542Google Scholar
  14. Flügge MU, Heldt HW (1991) Metabolite translocators of the chloroplast envelope. Ann Rev Plant Physiol Plant Molecular Biol 42:129–144CrossRefGoogle Scholar
  15. Foyer CH, Harbron S, Walker DA (1981) Regulation of sucrose phosphate synthetase and sucrose biosynthesis in spinach leaves. In: Akoyunoglou G (ed) (Proceed of the 5th Int Congr on Photosynthesis, Kassandra-Halkidiki), Photosynthesis Vol IV. Balaban International Science Services, Philadelphia, pp 357–364Google Scholar
  16. Foyer CH, Walker DA, Latzko E (1982) The regulation of cytoplasmic fructose 1,6-bisphosphatase in relation to the control of carbon flow to sucrose in leaves. Zeitschrift Pflanzenphysiol 107:457–466Google Scholar
  17. Fuller RC (1999) Forty years of microbial photosynthesis research: Where it came from and what it led to. Photosynth Res 62:1–29CrossRefGoogle Scholar
  18. Heldt HW, Rapley L (1970) Specific transport of inorganic phosphate, 3-phosphoglycerate dihydroxyacetonephosphate and of dicarboxylates across the inner envelope of spinach chloroplasts. FEBS Lett 10:143–148PubMedCrossRefGoogle Scholar
  19. Jensen RG, Bassham JA (1966) Photosynthesis by isolated chloroplasts. Proc Natl Acad Sci USA 56:1095–1101PubMedCrossRefGoogle Scholar
  20. Jensen RG, Bassham JA (1968) Photosynthesis by isolated chloroplasts III. Light activation of the carboxylation reaction. Biochim Biophys Acta 153:227–234PubMedCrossRefGoogle Scholar
  21. Kelly GJ, Latzko E (2006) Thirty Years of photosynthesis. Springer, Berlin, pp 1–414Google Scholar
  22. Leegood RC, Walker DA (1980) Autocatalysis and light activation of enzymes in relation to photosynthetic induction in wheat chloroplasts. Arch Biochem Biophys 200:575–582PubMedCrossRefGoogle Scholar
  23. Ochoa S, Veiga Salles JB, Ortiz PJ (1950) Biosynthesis of dicarboxylic acids by carbon dioxide fixation. iii. Enzymatic synthesis of malic acid by reductive carboxylation of pyruvic acid. J Biol Chem 187:863–874PubMedGoogle Scholar
  24. Osmond CM (1978) Crassulacean acid metabolism: A curiosity in context. Ann Rev Plant Physiol 29:379–414CrossRefGoogle Scholar
  25. Robinson SP, Walker DA (1979) The site of sucrose synthesis in isolated leaf protoplasts. FEBS Lett 107:295–299PubMedCrossRefGoogle Scholar
  26. Sachs J (1862) Ueber den Einfluss des Lichtes auf die Bildung des Amylums in den Chlorophyllkörnern. Bot Zeitung 20:365–373Google Scholar
  27. Stitt M, Heldt HW (1985) Control of photosynthetic sucrose synthesis by fructose 2,6-bisphosphate VI. Regulation of the cytosolic fructose 1,6-bisphosphatase in spinach leaves by an interaction between metabolic intermediates and fructose 2,6-bisphosphate. Plant Physiol 79:599–608PubMedGoogle Scholar
  28. Stokes DM, Walker DA, McCormick AV (1972) Photosynthetic oxygen evolution in a reconstituted chloroplast system. In: Forti G, Avron M, Melandri A (eds) Progress in photosynthesis (Proceed II Internat Congr on Photosynthesis, Stresa 1971). W Junk, NV Publisher, The Hague, pp 1779–1785Google Scholar
  29. Thomas M (1947) Plant physiology. Churchill, London, pp 1–504Google Scholar
  30. Walker DA (1956) Malate synthesis in a cell-free extract from a Crassulacean plant. Nature 178:593–594CrossRefGoogle Scholar
  31. Walker DA (1957) Physiological studies on acid metabolism. 4. Phosphoenolpyruvic carboxylase activity in extracts of Crassulacean plants. Biochem J 67:73–79PubMedGoogle Scholar
  32. Walker DA (1965) Correlation between photosynthetic activity and membrane integrity in isolated pea chloroplasts. Plant Physiol 40:1157–1161PubMedGoogle Scholar
  33. Walker DA, Cockburn W, Baldry CW (1967) Photosynthetic oxygen evolution by isolated chloroplasts in the presence of carbon cycle intermediates. Nature 216:597–599CrossRefGoogle Scholar
  34. Walker DA, Hill R (1967) The relation of oxygen evolution to carbon assimilation with isolated chloroplasts. Biochim Biophys Acta 131:330–333PubMedCrossRefGoogle Scholar
  35. Walker DA, Baldry CW, Cockburn W (1968) Photosynthesis by isolated chloroplasts, simultaneous measurement of carbon assimilation and oxygen evolution. Plant Physiol 43:1419–1422PubMedCrossRefGoogle Scholar
  36. Walker DA, Crofts AR (1970) Photosynthesis. Ann Rev Biochem 39:389–428PubMedCrossRefGoogle Scholar
  37. Walker DA (1972) The affinity of ribulose diphosphate carboxylasefor CO2/bicarbonate. In: Forti G, Avron M, Melandri A (eds) Progress in photosynthesis (Proceed internat congr on photosynthesis, Stresa 1971). W Junk, NV Publisher, The Hague, pp 1774–1778Google Scholar
  38. Walker DA, Leegood RC, Sivak MN (1986) Ribulose bisphosphate carboxylase-oxygenase: its role in photosynthesis. Phil Trans Roy Soc B 313:305–324CrossRefGoogle Scholar
  39. Walker DA, Lilley RMcC (1976) Ribulose bisphosphate carboxylase—an enigma resolved. In: Sunderland N (ed) Photosynthesis and physiology of the whole plant (Proceedings 50th Anniversary Meeting, S.E.B. Cambridge). Pergamon Press, Oxford, pp 189–198Google Scholar
  40. Walker DA (1992) Energy, Plants and Man, 2nd edn, (ISBN1870232 05 4). Oxygraphics, Brighton, 277ppGoogle Scholar
  41. Walker DA (2000) Like clockwork, (ISBN 1 870 232 12 7). Oxygraphics, Sheffield, 129ppGoogle Scholar
  42. Walker DA (2003) Chloroplasts in envelopes: CO2 fixation by fully functional intact chloroplasts. Photosynth Res 76:319–327PubMedCrossRefGoogle Scholar
  43. Walker DA (1973) Photosynthetic induction phenomena and the light activation of ribulose diphosphate carboxylase. New Phytol 72:209–235CrossRefGoogle Scholar
  44. Zeeman Samuel C, Smith Steven M, Smith Alison M (2004) The breakdown of starch in leaves. New Phytologist 163:247–261CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Robert Hill InstituteUniversity of SheffieldSheffieldUK

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