Photosynthesis Research

, Volume 89, Issue 1, pp 43–48

Binding of a Transition State Analog to Newly Synthesized Rubisco

Regular Paper

Abstract

Radioactive amino acids, when added to isolated pea chloroplasts or chloroplast extracts engaged in protein synthesis, are incorporated into Rubisco large subunits that co-migrate with native Rubisco during nondenaturing electrophoresis. We have added the transition state analog 2′-carboxyarabinitol bisphosphate (CABP) to chloroplast extracts after in organello or in vitro incorporation of radioactive amino acids into Rubisco large subunits. Upon addition of CABP the radioactive bands co-migrating with native Rubisco undergo a readily detected shift in electrophoretic mobility just as the native enzyme, thus demonstrating the ability of the newly assembled molecules to interact with this transition state analog.

Keywords

Chaperonin Rubisco Protein synthesis Assembly 

Abbreviations

CABP

2-carboxyarabinitol 1,5-bisphosophate

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Boovaraghan Balaji
    • 1
    • 2
  • Michele Gilson
    • 1
  • Harry Roy
    • 1
  1. 1.Biology DepartmentRensselaer Polytechnic InstituteTroyUSA
  2. 2.Division of Plant SciencesUniversity of MissouriColumbiaUSA

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