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Photosynthesis Research

, Volume 85, Issue 3, pp 341–357 | Cite as

Dynamics of Fluxes Through Photosynthetic Complexes in Response to Changing Light and Inorganic Carbon Acclimation in Synechococcus elongatus

  • Tyler D.B. MacKenzie
  • Jeanette M. Johnson
  • Douglas A. CampbellEmail author
Regular paper

Abstract

Cyanobacteria acclimate to environmental inorganic carbon (Ci) concentrations through re-organisations of photosynthetic function and the induction of carbon concentrating mechanisms (CCMs), which alter and constrain their subsequent acclimation to changing light. We grew cells acclimated to high Ci (4 mM) or low Ci (0.02 mM), shifted them from 50 μmol m−2 s−1 to 500 μmol m−2 s−1, and quantified their photosynthetic performance in parallel with quantitation of allocations to key indicator macromolecules. Pigments cell−1 declined, PsbA (PS II), AtpB (ATP Synthase), RbcL (Rubisco) and GlnA (Glutamine Synthetase) increased, and PsaC (PS I) remained stable through the light shift. The increase in these protein pools was slower and smaller in low Ci cells, but acted in both cell types to re-normalise the electron fluxes through the catalytic complexes back toward values before the light shift (for PsbA and GlnA) or even below the initial flux per complex (for RbcL). In contrast, an increased electron flux per PsaC was sustained for at least 6 h after the increase in light. Initially, high levels of PS II cell−1 and PS II connectivity in high Ci cells caused a more rapid net photoinactivation of PS II in high Ci cells than in low Ci cells, depressing the rate of PS II-specific electron transport (PS II ETR) to levels similar to linear ETR (net O2 evolution minus respiration). In low Ci cells, PS II ETR remained in excess of linear ETR and may have helped maintain CCM activity. The pool sizes of PsbA, AtpB and GlnA correlated with cellular growth rate, and changed at similar rates in high Ci and low Ci cells when expressed on a generational rather than chronological timescale, which has implications for differing ecology of high and low Ci cells under variable natural light.

Key words:

ATP Synthase CCM light acclimation photosynthetic electron transport protein quantitation PS I PS II Rubisco Synechococcus elongatus PCC 7942 

Abbreviations

CCM

carbon concentrating mechanism

Ci

inorganic carbon concentration

ETR

electron transport rate

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Copyright information

© Springer 2005

Authors and Affiliations

  • Tyler D.B. MacKenzie
    • 1
  • Jeanette M. Johnson
    • 2
  • Douglas A. Campbell
    • 2
    Email author
  1. 1.Department of BiologyUniversity of New BrunswickFrederictonCanada
  2. 2.Department of Biology and Coastal Wetlands InstituteMount Allison UniversityCanada

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