Photosynthesis Research

, Volume 86, Issue 1–2, pp 203–216 | Cite as

Spectral and Kinetic Analysis of the Energy Coupling in the PS I–LHC I Supercomplex from the Green Alga Chlamydomonas reinhardtii at 77 K

  • Alexander N. Melkozernov
  • Joanna Kargul
  • Su Lin
  • James Barber
  • Robert E. Blankenship
Regular Paper

Abstract

Energy transfer processes in the chlorophyll antenna of the PS I–LHCI supercomplexes from the green alga Chlamydomonas reinhardtii have been studied at 77 K using transient absorption spectroscopy with multicolor excitation in the 640–670 nm region. Comparison of the kinetic data obtained at low and room temperatures indicates that the slow ∼ ∼100 ps excitation equilibration phase that is characteristic of energy coupling of the LHCI peripheral antenna to the PS I core at physiological temperatures (Melkozernov AN, Kargul J, Lin S, Barber J and Blankenship RE (2004) J Phys Chem B 108: 10547–10555) is not observed in the excitation dynamics of the PS I–LHCI supercomplex at 77 K. This suggests that at low temperatures the peripheral antenna is energetically uncoupled from the PS I core antenna. Under these conditions the observed kinetic phases on the time scales from subpicoseconds to tens of picoseconds represent the superposition of the processes occurring independently in the PS I core antenna and the Chl a/b containing LHCI antenna. In the PS I–LHCI supercomplex with two uncoupled antennas the excitation is channeled to the excitation sinks formed at low temperature by clusters of red pigments. A better spectral resolution of the transient absorption spectra at 77 K results in detection of two ΔA bands originating from the rise of photobleaching on the picosecond time scale of two clearly distinguished pools of low energy absorbing Chls in the PS I–LHCI supercomplex. The first pool of low energy pigments absorbing at 687 nm is likely to originate from the red pigments in the LHCI where the Lhca1 protein is most abundant. The second pool at 697 nm is suggested to result either from the structural interaction of the LHCI and the PS I core or from other Lhca proteins in the antenna. The kinetic data are discussed based on recent structural models of the PS I–LHCI. It is proposed that the uncoupling of pigment pools may be a control mechanism that regulates energy flow in Photosystem I.

Keywords

core antenna excited state dynamics green algae LHCI peripheral antenna nonphotochemical trapping photochemical trapping photosynthesis Photosystem I PS I–LHCI supercomplex transient absorption spectroscopy red pigments 

Abbreviations

ΔA

absorption difference

Chl

chlorophyll

DAS

decay associated spectrum

fwhm

full width at half maximum

LHCI

Chl a/bcontaining light-harvesting complex of Photosystem I

PB

photobleaching

PS I

Photosystem I

RC

reaction center

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Copyright information

© Springer 2005

Authors and Affiliations

  • Alexander N. Melkozernov
    • 1
  • Joanna Kargul
    • 2
  • Su Lin
    • 1
  • James Barber
    • 2
  • Robert E. Blankenship
    • 1
  1. 1.Department of Chemistry and BiochemistryCenter for the Study of Early Events in PhotosynthesisTempeUSA
  2. 2.Department of Biological Sciences,Wolfson LaboratoriesImperial CollegeUK

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