Photosynthesis Research

, Volume 84, Issue 1–3, pp 297–302 | Cite as

Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of Photosystem II

  • Frantisek Vacha
  • Jakub Psencik
  • Michal Kuty
  • Milan Durchan
  • Pavel Siffel
Regular paper

Abstract

Absorption and circular dichroism spectra of Photosystem II (PS II) reaction centres (RC) were studied and compared with spectra calculated on the basis of point-dipole approximation. Chlorophyll cation was accumulated during a light treatment of PS II RC in the presence of artificial electron acceptor silicomolybdate. Light-induced difference spectra and their calculated counterparts revealed the location of accumulated cation at the accessory chlorophyll of the D1 protein subunit.

Keywords

 charge separation chlorophyll photosynthesis Photosystem II reaction centre 

Abbreviations

CD

circular dichroism

Chl

chlorophyll

D1

psbA gene product

D2

psbD gene product

DM

n-dodecyl-β-D-maltoside

FWHM

full-width at half maximum

MES

morpholinethanesulfonic acid

Pheo

pheophytin

PS II

Photosystem II

RC

reaction centre

SiMo

silicomolibdic acid

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Copyright information

© Springer 2005

Authors and Affiliations

  • Frantisek Vacha
    • 1
    • 2
  • Jakub Psencik
    • 3
    • 1
  • Michal Kuty
    • 1
    • 4
  • Milan Durchan
    • 1
    • 2
  • Pavel Siffel
    • 1
    • 2
  1. 1.Institute of Physical BiologyUniversity of South BohemiaNove HradyCzech Republic
  2. 2.Institute of Plant Molecular BiologyAcademy of Sciences of the Czech RepublicCeske BudejoviceCzech Republic
  3. 3.Faculty of Mathematics and PhysicsCharles UniversityPragueCzech Republic
  4. 4.Institute of Landscape EcologyAcademy of Sciences of the Czech RepublicNove HradyCzech Republic

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