Photosynthesis Research

, Volume 84, Issue 1–3, pp 297–302 | Cite as

Evidence for localisation of accumulated chlorophyll cation on the D1-accessory chlorophyll in the reaction centre of Photosystem II

  • Frantisek Vacha
  • Jakub Psencik
  • Michal Kuty
  • Milan Durchan
  • Pavel Siffel
Regular paper


Absorption and circular dichroism spectra of Photosystem II (PS II) reaction centres (RC) were studied and compared with spectra calculated on the basis of point-dipole approximation. Chlorophyll cation was accumulated during a light treatment of PS II RC in the presence of artificial electron acceptor silicomolybdate. Light-induced difference spectra and their calculated counterparts revealed the location of accumulated cation at the accessory chlorophyll of the D1 protein subunit.


 charge separation chlorophyll photosynthesis Photosystem II reaction centre 



circular dichroism




psbA gene product


psbD gene product




full-width at half maximum


morpholinethanesulfonic acid




Photosystem II


reaction centre


silicomolibdic acid


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Copyright information

© Springer 2005

Authors and Affiliations

  • Frantisek Vacha
    • 1
    • 2
  • Jakub Psencik
    • 3
    • 1
  • Michal Kuty
    • 1
    • 4
  • Milan Durchan
    • 1
    • 2
  • Pavel Siffel
    • 1
    • 2
  1. 1.Institute of Physical BiologyUniversity of South BohemiaNove HradyCzech Republic
  2. 2.Institute of Plant Molecular BiologyAcademy of Sciences of the Czech RepublicCeske BudejoviceCzech Republic
  3. 3.Faculty of Mathematics and PhysicsCharles UniversityPragueCzech Republic
  4. 4.Institute of Landscape EcologyAcademy of Sciences of the Czech RepublicNove HradyCzech Republic

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