Photosynthesis Research

, Volume 83, Issue 1, pp 45–52

Resonance Raman spectroscopy of carotenoids in Photosystem II core complexes

  • Cara A. Tracewell
  • Agnes Cua
  • David F. Bocian
  • Gary W. Brudvig
Regular paper

Abstract

Resonance Raman (RR) spectroscopy has been used to examine the configuration of the carotenoids bound to Synechocystis PCC 6803 Photosystem II (PS II) core complexes. The excitation wavelengths used (514.5, 488.0, 476.5 and 457.9 nm) span the absorption bands of all of the ~12–17 neutral carotenoids in the PS II core complex. The RR spectra of the two carotenoids associated with the D1–D2 polypeptides (Car507 and Car489) of the reaction center are extracted via light versus dark difference experiments measured at 20 K. The RR results are consistent with all-trans configurations for both Car507 and Car489 and indicate that majority of the other carotenoids in the PS II core complex must also be in the all-trans configuration. The configuration of β-carotene is relevant to its proposed function as a molecular wire in the secondary electron-transfer reactions of PS II.

Keywords

β -carotene photosystem II resonance Raman secondary electron transfer pathway 

Abbreviations

Car507 and Car489

carotenoids associated with the D1–D2 polypeptides of the reaction center

Chl

chlorophyll

CP43

chlorophyll-binding protein of molecular weight 43 kDa

CP47

chlorophyll-binding protein of molecular weight 47 kDa

Cyt

cytochrome

β-DM

β-dodecyl maltoside

HPLC

high pressure liquid chromatography

MES

2-(N-morpholino)ethanesulphonic acid

PS II

Photosystem II

RC

reaction center

RR

resonance Raman

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Copyright information

© Springer 2005

Authors and Affiliations

  • Cara A. Tracewell
    • 1
  • Agnes Cua
    • 2
  • David F. Bocian
    • 2
  • Gary W. Brudvig
    • 1
  1. 1.Department of ChemistryYale UniversityNew HavenUSA
  2. 2.Department of ChemistryUniversity of CaliforniaRiversideUSA

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