Transcriptomic Analyses of Chilling Stress Responsiveness in Leaves of Tobacco (Nicotiana tabacum) Seedlings

  • Peilu Zhou
  • Rayyan Khan
  • Qiyao Li
  • Guangliang Liu
  • Na Xu
  • Yinju Yang
  • Yi Wang
  • Shusheng WangEmail author
  • Aiguo ChenEmail author
Original Paper


Low temperature is among the most significant abiotic stresses restricting geographical distribution of plants and reducing crop productivity. However, the molecular regulatory mechanisms of tobacco plants in response to low temperature are poorly understood. To elucidate the molecular mechanisms of chilling tolerance in tobacco, the transcriptomic responses of tobacco under chilling stress were analyzed using RNA-seq analysis. A total of 1675 differentially expressed genes (DEGs) were detected from T12h vs. CK12h and T24h vs. CK24h libraries; among these genes, 1170 genes were upregulated and 505 were downregulated. Additionally, 109 genes were found to be specifically expressed in tobacco seedlings under chilling stress. Functional annotation revealed that the DEGs enriched that categories of regulating soluble sugar and polyamine content and composition to maintain cell osmotic potential, accelerating the de novo synthesis of D1 protein to promote PSII repair, regulating signal transduction such as ABA and GA, and promoting lipid metabolism and lignin synthesis to improve stability of membrane system and mechanical strength of cell wall. This work provides additional insights into the molecular basis of tobacco seedling responses to low-temperature stress.


Chilling stress Tobacco (Nicotiana tabacumTranscriptome analysis Gene expression 



We thank LetPub ( for its linguistic assistance during the preparation of this manuscript.

Funding Information

This research was funded by Fundamental Research Funds for Central Non-Profit Scientific Institution, grant number 1610232016019, and Agricultural Science and Technology Innovation Program, grant number ASTIP-TRIC03.

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflict of interest.

Supplementary material

11105_2019_1167_MOESM1_ESM.docx (15 kb)
S1 Table . List of primers used for qRT-PCR analysis (MSWord). (DOCX 14 kb)
11105_2019_1167_MOESM2_ESM.xls (78 kb)
S2 Table . KEGG annotation analysis of upregulated DEGs responded to chilling (MSexcle). (XLS 78 kb)
11105_2019_1167_MOESM3_ESM.xls (42 kb)
S3 Table . KEGG annotation analysis of downregulated DEGs responded to chilling (MSexcle). (XLS 41 kb)
11105_2019_1167_MOESM4_ESM.xls (91 kb)
S4 Table . Candidate genes involved in tobacco chilling tolerance (MSexcle). (XLS 91 kb)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Peilu Zhou
    • 1
    • 2
  • Rayyan Khan
    • 1
  • Qiyao Li
    • 1
  • Guangliang Liu
    • 1
  • Na Xu
    • 1
  • Yinju Yang
    • 1
  • Yi Wang
    • 1
  • Shusheng Wang
    • 1
    Email author
  • Aiguo Chen
    • 1
    Email author
  1. 1.Key Laboratory of Tobacco Biology and Processing, Ministry of AgricultureTobacco Research Institute of Chinese Academy of Agricultural SciencesQingdaoPeople’s Republic of China
  2. 2.College of Agronomy, Resources and EnvironmentTianjin Agricultural UniversityTianjinPeople’s Republic of China

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