Plant Molecular Biology Reporter

, Volume 36, Issue 5–6, pp 878–887 | Cite as

Inheritance and Molecular Marker for Flowering Time in Radish (Raphanus sativus L.)

  • Qingbiao Wang
  • Yanping Wang
  • Li ZhangEmail author
Original Paper


The mixed inheritance model involving major genes and polygenes was used to analyze the inheritance of radish flowering time trait in the B1, B2, F1, and F2 generations. Our results showed that flowering time was regulated by two additive-dominant-epistatic major genes and additive-dominant-epistatic polygenes (E-0 model). The major gene heritability estimated for the B1, B2, and F2 generations was 70.48%, 82.80%, and 86.90%, respectively, while the polygene heritability estimated was 0.00, 12.58%, and 8.19%, respectively. These results suggested that the flowering time of radish was regulated by major genes, with polygenes playing only a minor role. In practice, high heritability of major genes is favorable for an efficient selection of B2 and F2 generations during radish breeding. Six hundred twenty-six SSR markers were screened between late-bolting bulk and the early bolting bulk from F2 population. A marker RSS0119 associated to the flowering time trait was obtained, which revealed a high correlation with the flowering time in the F2 population as well as in 59 radish inbred lines (83.61% and 61.02%, respectively). Our findings will be useful for breeding late-bolting varieties.


Radish Flowering time Major gene and polygene genetic model Molecular marker 


Author Contributions

ZL and WQ designed and managed the methods and experiments, performed data analysis, and wrote the manuscript. WY wrote and revised the manuscript. All authors read and approved the final manuscript.

Funding information

This work was funded by grants from the National key R&D Program of China (2017YFD0101806), Foundation for Young Scientists of Beijing Academy of Agricultural and Forestry Sciences (QNJJ201503), the Innovative Team Construction Project of BAAFS (JNKYT201601), and the Technological Innovation Capacity Program of BAAFS (KJCX20170710 and KJCX20170102).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. Cao XW, Liu B, Zhang YM (2013) SEA: a software package of segregation analysis of quantitative traits in plants. J Nanjing Agric Univ 36(6):1–6 in Chinese with English abstractGoogle Scholar
  2. Gai JY, Zhang YM, Wang JK (2003) Genetic system of quantitative traits in plants. Science Press, Beijing, pp 120–126Google Scholar
  3. Ji XH, Yin L, Shen BY, Zhang L, Wang YG, Feng H (2013) Inheritance analysis of bolting correlated traits using mixed major gene plus polygene model in Brassica rapa. Chin Agric Sci Bull 29(04):76–82 in Chinese with English abstractGoogle Scholar
  4. Kakizaki T, Kato T, Fukino N, Fukino N, Ishida M, Hatakeyama K, Matsumoto S (2011) Identification of quantitative trait loci controlling late bolting in Chinese cabbage (Brassica rapa L.) parental line Nou 6 gou. Breed Sci 159:151–159CrossRefGoogle Scholar
  5. Kang ES, Ha SM, Ko HC, Yu HJ, Chae WB (2016) Reproductive traits and molecular evidence related to the global distribution of cultivated radish (Raphanus sativus L.). Plant Syst Evol 302:1367–1380CrossRefGoogle Scholar
  6. Kitamoto N, Yui S, Nishikawa K, Takahata Y, Yokoi S (2014) A naturally occurring long insertion in the first intron in the Brassica rapa FLC2 gene causes delayed bolting. Euphytica 196:213–223CrossRefGoogle Scholar
  7. Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft sequences of the radish (Raphanus sativus L.) genome. DNA Res 21:481–490CrossRefGoogle Scholar
  8. Li M (2009) Inheritance and mapping QTL and physiology research on bolting and flowering characters in cabbage (Brassica oleracea var. capitata L.). Chinese academy of agriculture science, BeijingGoogle Scholar
  9. Li XX, Shen D (2007) Descriptors and data standards for radish (Raphanus sativus L.). China Agric. Press, p 66 in ChineseGoogle Scholar
  10. Li XF, Zhu HF, Zhu YY, Hou RX, Zhai W (2016) Inheritance of major gene plus polygene underlying bolting and flowering traits in Pak-choi. J Nucl Agric Sci 30(12):2318–2325 in Chinese with English abstractGoogle Scholar
  11. Liu LW, Zhao LP, Gong YQ, Wang MX (2008) DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers. Sci Hortic 116:240–247CrossRefGoogle Scholar
  12. Lou P, Zhao JJ, Kim JS, Kim JS, Shen SX, Del Carpio DP, Song XF, Jin MN, Vreugdenhil D, Wang XW, Koornneef M, Bonnema G (2007) Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa. J Exp Bot 58:4005–4016CrossRefGoogle Scholar
  13. Mitsui Y, Shimomura M, Komatsu K, Namiki N, Shibata-Hatta M, Imai M, Katayose Y, Mukai Y, Kanamori H, Kurita K, Kagami T, Wakatsuki A, Ohyanagi H, Ikawa H, Minaka N, Nakagawa K, Shiwa Y, Sasaki T (2015) The radish genome and comprehensive gene expression profile of tuberous root formation and development. Sci Rep 5:10835CrossRefGoogle Scholar
  14. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucl Acids Res 8:4321–4325CrossRefGoogle Scholar
  15. Okazaki K, Sakamoto K, Kikuchi R, Saito A, Togashi E, Kuginuki Y, Matsumoto S, Hirai M (2007) Mapping and characterization of FLC homologs and QTL analysis of flowering time in Brassica oleracea. Theor Appl Genet 114:595–608CrossRefGoogle Scholar
  16. Qi ZY, Li JX, Raza MA, Zou XX, Cao LW, Rao LL, Chen LP (2015) Inheritance of fruit cracking resistance of melon (Cucumis melo L.) fitting E-0 genetic model using major gene plus polygene inheritance analysis. Sci Hortic 189:168–174CrossRefGoogle Scholar
  17. Schranz ME, Quijada P, Sung SB, Lukens L, Amasino R, Osborn TC (2002) Characterization and effects of the replicated flowering time gene FLC in Brassica rapa. Genetics 162:1457–1468PubMedPubMedCentralGoogle Scholar
  18. Shinohara S (1959) Genealogical studies on the phasic development of flowering centering on the cruciferous crops, especially on the role of vernalization on ripening seeds. Spec Bull Shizuoka Agric Exp Stn 6:1–39Google Scholar
  19. Shirasawa K, Oyama M, Hirakawa H, Sato S, Tabata S, Fujioka T, Kimizuka-Takagi C, Sasamoto S, Watanabe A, Kato M, Kishida Y, Kohara M, Takahashi C, Tsuruoka H, Wada T, Sakai T, Isobe S (2011) An EST-SSR linkage map of Raphanus sativus and comparative genomics of the Brassicaceae. DNA Res 18:221–232CrossRefGoogle Scholar
  20. Shu JS, Liu YM, Li ZS, Zhang LL, Fang ZY, Yang LM, Zhuang M, Zhang YY, Lv H (2015) Genetic analysis of floral organ size in broccoli×cabbage via a mixed inheritance model of a major gene plus polygene. Genet Mol Res 15(2):15027554Google Scholar
  21. Wang L, He Q (2005) China radish. Scientific and Technical Documents Publishing House, BeijingGoogle Scholar
  22. Wang WY, Zhang F, Yu XY, Shen XL, Ge YY, Zhang Z (2012) Genetic analysis and associated SRAP markers for horticultural traits of Aechmea bromeliads. Sci Hortic 147:29–33CrossRefGoogle Scholar
  23. Wang CC, Zhang F, Guan ZY, Chen SM, Jiang JF, Fang WM, Chen FD (2014) Inheritance and molecular markers for aphid (Macrosiphoniella sanbourni) resistance in chrysanthemum (Chrysanthemum morifolium Ramat.). Sci Hortic 180:220–226CrossRefGoogle Scholar
  24. Wang QB, Zhang L, Zheng PJ (2015) Genetic diversity and evolutionary relationship analyses within and among Raphanus species using EST-SSR markers. Mol Breed 35(62):1–12Google Scholar
  25. Xu WL, Wang SF, Mu JH, Wang CH, Liu XX (2007) Identification of AFLP and SCAR molecular markers linked to bolting trait in radish. Mol Plant Breed 9(7):743–749 in Chinese with English abstractGoogle Scholar
  26. Yi G, Park H, Kim JS, Chae WB, Park S, Huh JH (2014) Identification of three FLOWERING LOCUS C genes responsible for vernalization response in radish (Raphanus sativus L.). Hort Environ Biotechnol 55:548–556CrossRefGoogle Scholar
  27. Yuan YX, Wu J, Sun RF, Zhang XW, Xu DH, Bonnema G, Wang XW (2009) A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time. J Exp Bot 60:1299–1308CrossRefGoogle Scholar
  28. Yuasa I (1988) Some problems on the breeding of late bolting, 3: breeding of the late bolting (2): radish. Jpn Soc Seed Prod Breed Eng:37–44Google Scholar
  29. Zhang YM, Gai JY (2000) The IECM algorithm for estimation of component distribution parameters in segregating analysis of quantitative traits. Acta Agron Sin 26:699–706Google Scholar
  30. Zhang YM, Gai JY, Yang YH (2003) The EIM algorithm in the joint segregation analysis of quantitative traits. Genet Res 81:157–163CrossRefGoogle Scholar
  31. Zhao LP (2007) Genetic analysis of bolting and cultivar fingerprinting in radish (Rapanus sativus L.). Nanjing Agricul. Univ, Nanjing in Chinese with English abstractGoogle Scholar
  32. Zhao JJ, Kulkarni V, Liu NN, Del Carpio DP, Bucher J, Bonnema G (2010) BrFLC2 (FLOWERING LOCUS C) as a candidate gene for a vernalization response QTL in Brassica rapa. J Exp Bot 61:4005–4016CrossRefGoogle Scholar
  33. Zhuo ZC, Wang EM, Zhang LG, Zhang MK, Hui MX (2009) Major gene plus polygene inheritance analysis of bolting trait in heading Chinese cabbage. Acta Bot Boreali-Occiden Sin 29(5):0923–0928 in Chinese with English abstractGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry SciencesBeijingChina
  2. 2.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China)BeijingChina
  3. 3.Ministry of Agriculture, China; Beijing Key Laboratory of Vegetable Germplasm ImprovementBeijingChina

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