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Molecular Mechanisms Endowing Cross-resistance to ALS-Inhibiting Herbicides in Amaranthus hybridus from Argentina

  • Alvaro S. Larran
  • Florencia Lorenzetti
  • Daniel Tuesca
  • Valeria E. Perotti
  • Hugo R. Permingeat
Short Communication
  • 103 Downloads

Abstract

Amaranthus hybridus L. is one of the most problematic weeds in summer crops in Argentina. However, 20 years after the detection of the first case of resistance to ALS-inhibiting herbicides in this country, no extensive reports of the molecular mechanisms endowing resistance were published. In this work, we sequenced the acetolactate synthase gene of resistant plants belonging to five different populations of A. hybridus from Santa Fe and Cordoba provinces. We found that every population presented at least one of the previously documented substitutions W574L and D376E in ALS amino acid sequence. These results explain the cross-resistance to ALS-inhibiting herbicides and should alert about the usage of herbicides with a different site of action after an ineffective control of this species. This is the first report of these target-site mechanisms endowing resistance to ALS-inhibiting herbicides in A. hybridus populations from Argentina.

Keywords

A. quitensis A. hybridus Weeds Herbicide resistance Acetolactate synthase Weed control 

Notes

Acknowledgements

The authors are grateful to Dr. Lucas Lieber for advising during the first stage of the research. ASL and FL are fellows of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), HRP is Professor of the Universidad Nacional de Rosario (UNR), and researcher of IICAR-CONICET and VEP is a Professor of the Universidad Nacional de Rosario (UNR).

Author Contributions

ASL wrote the manuscript. ASL and FL both conducted the molecular experiments in equal parts. DT collected the material and conducted the spraying experiments. VEP and HRP guided the research and corrected the manuscript. All authors read and approved the manuscript.

Funding

This study was funded by SECTEI-Santa Fe (Resolution 119/2015-Project 2010-149-14). The funding source had no involvement in study design.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Ethical Approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Beckie HJ, Tardif FJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:15–28CrossRefGoogle Scholar
  2. Berger S, Madeira PT, Ferrell J, Gettys L, Morichetti S, Cantero JJ, Nunez C (2016) Palmer amaranth (Amaranthus palmeri) identification and documentation of ALS-resistance in Argentina. Weed Sci 64:312–320CrossRefGoogle Scholar
  3. Duke SO (2011) Why are there no new herbicide modes of action in recent years? Pest Manag Sci 68:505–512CrossRefGoogle Scholar
  4. Heap I (2018) The international survey of herbicide resistant weeds. http://www.weedscience.org. Accessed 20 Jul 2018
  5. Larran AS, Palmieri VE, Perotti VE, Lieber L, Tuesca D, Permingeat HR (2017) Target-site resistance to ALS-inhibiting herbicides in Amaranthus palmeri from Argentina. Pest Manag Sci 73:2578–2484CrossRefGoogle Scholar
  6. Maertens KD, Sprague CL, Tranel PJ, Hines RA (2004) Amaranthus hybridus populations resistant to triazine and acetolactate synthase-inhibiting herbicides. Weed Res 44:21–26CrossRefGoogle Scholar
  7. Morichetti S, Cantero JJ, Núñez C, Barboza GE, Amuchastegui A, Ferrell J (2013) On the presence of Amaranthus palmeri (Amaranthaceae) in Argentina. Bol Soc Arg Bot 48:347–353Google Scholar
  8. Owen MDK (2016) Diverse approaches to herbicide-resistant weed management. Weed Sci 64:570–584CrossRefGoogle Scholar
  9. REM (2018) Red de conocimiento de malezas resistentes. https://www.aapresid.org.ar/rem. Accessed 20 Jul 2018
  10. Romagnoli MV, Tuesca D, Permingeat HR (2013) Characterization of Amaranthus quitensis resistance to three families of herbicides. Ecol Austral 23:119–125Google Scholar
  11. Scursoni JA, Satorre EH (2010) Glyphosate management strategies, weed diversity and soybean yield in Argentina. Crop Prot 29:957–962CrossRefGoogle Scholar
  12. Shaner DL (2014) Lessons learned from the history of herbicide resistance. Weed Sci 62:427–431CrossRefGoogle Scholar
  13. Tardif FJ, Rajcan I, Costea M (2006) A mutation in the herbicide target site acetohydroxyacid synthase produces morphological and structural alterations and reduces fitness in Amaranthus powellii. New Phytol 169:251–264CrossRefGoogle Scholar
  14. Tranel P, Wassom J, Jeschke M, Rayburn A (2002) Transmission of herbicide resistance from a monoecious to a dioecious weedy Amaranthus species. Theor Appl Genet 105:674–679CrossRefGoogle Scholar
  15. Trucco F, Hager AG, Tranel PJ (2006) Acetolactate synthase mutation conferring imidazolinone-specific herbicide resistance in Amaranthus hybridus. J Plant Physiol 163:475–479CrossRefGoogle Scholar
  16. Tuesca D, Nisensohn L (2001) Resistance of Amaranthus quitensis to imazethapyr and clhorimuron-ethyl. Pesq Agrop Bras 36:601–606CrossRefGoogle Scholar
  17. Vila-Aiub MM, Gundel PE, Preston C (2015) Experimental methods for estimation of plant fitness costs associated with herbicide-resistance genes. Weed Sci 63:203–216CrossRefGoogle Scholar
  18. Whaley CM, Wilson HP, Westwood JH (2006) ALS resistance in several smooth pigweed (Amaranthus hybridus) biotypes. Weed Sci 54:828–832CrossRefGoogle Scholar
  19. Whaley CM, Wilson HP, Westwood JH (2007) A new mutation in plant Als confers resistance to five classes of Als-inhibiting herbicides. Weed Sci 55:83–90CrossRefGoogle Scholar
  20. Yu Q, Han H, Li M, Purba E, Walsh MJ, Powles SB (2012) Resistance evaluation for herbicide resistance-endowing acetolactate synthase (ALS) gene mutations using Raphanus raphanistrum populations homozygous for specific ALS mutations. Weed Res 52:178–186CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratorio de Biología Molecular, Facultad de Ciencias AgrariasUniversidad Nacional de RosarioZavallaArgentina
  2. 2.Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR), Facultad de Ciencias AgrariasUniversidad Nacional de RosarioZavallaArgentina
  3. 3.Cátedra de Malezas, Facultad de Ciencias AgrariasUniversidad Nacional de RosarioZavallaArgentina

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