Molecular Mechanisms Endowing Cross-resistance to ALS-Inhibiting Herbicides in Amaranthus hybridus from Argentina
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Amaranthus hybridus L. is one of the most problematic weeds in summer crops in Argentina. However, 20 years after the detection of the first case of resistance to ALS-inhibiting herbicides in this country, no extensive reports of the molecular mechanisms endowing resistance were published. In this work, we sequenced the acetolactate synthase gene of resistant plants belonging to five different populations of A. hybridus from Santa Fe and Cordoba provinces. We found that every population presented at least one of the previously documented substitutions W574L and D376E in ALS amino acid sequence. These results explain the cross-resistance to ALS-inhibiting herbicides and should alert about the usage of herbicides with a different site of action after an ineffective control of this species. This is the first report of these target-site mechanisms endowing resistance to ALS-inhibiting herbicides in A. hybridus populations from Argentina.
KeywordsA. quitensis A. hybridus Weeds Herbicide resistance Acetolactate synthase Weed control
The authors are grateful to Dr. Lucas Lieber for advising during the first stage of the research. ASL and FL are fellows of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), HRP is Professor of the Universidad Nacional de Rosario (UNR), and researcher of IICAR-CONICET and VEP is a Professor of the Universidad Nacional de Rosario (UNR).
ASL wrote the manuscript. ASL and FL both conducted the molecular experiments in equal parts. DT collected the material and conducted the spraying experiments. VEP and HRP guided the research and corrected the manuscript. All authors read and approved the manuscript.
This study was funded by SECTEI-Santa Fe (Resolution 119/2015-Project 2010-149-14). The funding source had no involvement in study design.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Heap I (2018) The international survey of herbicide resistant weeds. http://www.weedscience.org. Accessed 20 Jul 2018
- Morichetti S, Cantero JJ, Núñez C, Barboza GE, Amuchastegui A, Ferrell J (2013) On the presence of Amaranthus palmeri (Amaranthaceae) in Argentina. Bol Soc Arg Bot 48:347–353Google Scholar
- REM (2018) Red de conocimiento de malezas resistentes. https://www.aapresid.org.ar/rem. Accessed 20 Jul 2018
- Romagnoli MV, Tuesca D, Permingeat HR (2013) Characterization of Amaranthus quitensis resistance to three families of herbicides. Ecol Austral 23:119–125Google Scholar