Expression Analysis of TCP Genes in Peach Reveals an Involvement of PpTCP.A2 in Ethylene Biosynthesis During Fruit Ripening
Plant-specific TCP transcription factor has been studied in plant growth and development, but little is known the roles during fruit development and ripening. Based on phylogenetic analysis, in this study, the 20 TCP genes isolated from peach were divided into two classes (I and II) comprised by four groups (A → D), and distributed into 19 clusters. Expression profiles of TCP genes in peach were analyzed to evaluate their potential roles during fruit development and ripening. The result showed that there are four genes (PpTCP.A2, PpTCP.A10, PpTCP.B1, and PpTCP.C1) and each gene had similar expression patterns detected by qRT-PCR to that tested by RNA-Seq, and higher expressed in fruitlets than in other developmental and ripening fruits, suggesting that these four genes were probably related to early development in peach fruit. Noteworthy, PpTCP.A2 was lower expressed in ripening fruits than in developmental or pre-ripening fruits of 11 peach cultivars, and virus-induced silencing PpTCP.A2 increased ethylene production and promoted PpACS1, but not PpACO1 and PpendoPGM expression. This result indicates that PpTCP.A2 was a transcription repressor, which could regulate ethylene biosynthesis by affecting PpACS1 expression, and thus should be involved in fruit ripening.
KeywordsPeach TCP transcription factor Fruit ripening Virus-induced gene silencing Phylogenetic classification
TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL
PROLIFERATING CELL FACTOR
Fragaria × ananassa
Endopolygalacturonase-mediating melting flesh phenotype
The work was supported by the National Natural Science Foundations of China (31471856 and 31672118), the Natural Science Foundation of Jiangsu Province (BK20171380), and the Fundamental Research Funds for the Central Universities (KYZ201732).
- Feng XZ, Zhao Z, Tian ZX, Xu SL, Luo YH, Cai ZG, Wang YM, Yang J, Wang Z, Weng L, Chen JH, Zheng LY, Guo XZ, Luo JH, Sato SS, Tabata S, Ma W, Cao XL, Hu XH, Sun CR, Luo D (2006) Control of petal shape and floral zygomorphy in Lotus japonicus. Proc Natl Acad Sci U S A 103:4970–4975CrossRefPubMedPubMedCentralGoogle Scholar
- Giraud E, Ng S, Carrie C, Duncan O, Low J, Lee CP, Van Aken O, Millar AH, Murcha M, Whelan J (2010) TCP transcription factors link the regulation of genes encoding mitochondrial proteins with the circadian clock in Arabidopsis thaliana. Plant Cell 22:3921–3934CrossRefPubMedPubMedCentralGoogle Scholar
- Herve C, Dabos P, Bardet C, Jauneau A, Auriac MC, Ramboer A, Lacout F, Tremousaygue D (2009) In vivo interference with AtTCP20 function induces severe plant growth alterations and deregulates the expression of many genes important for development. Plant Physiol 149:1462–1477CrossRefPubMedPubMedCentralGoogle Scholar
- Osorio S, Alba R, Damasceno CMB, Lopez-Casado G, Lohse M, Zanor MI, Tohge T, Usadel B, Rose JKC, Fei ZJ, Giovannoni JJ, Fernie AR (2011) Systems biology of tomato fruit development: combined transcript, protein, and metabolite analysis of tomato transcription factor (nor, rin) and ethylene receptor (nr) mutants reveals novel regulatory interactions. Plant Physiol 157:405–425CrossRefPubMedPubMedCentralGoogle Scholar
- Steiner E, Efroni I, Gopalraj M, Saathoff K, Tseng TS, Kieffer M, Eshed Y, Olszewski N, Weiss D (2012) The Arabidopsis O-linked N-acetylglucosamine transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers. Plant Cell 24:96–108CrossRefPubMedPubMedCentralGoogle Scholar