The Novel Rose MYB Transcription Factor RhMYB96 Enhances Salt Tolerance in Transgenic Arabidopsis
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MYB transcription factors play diverse roles in plant development and responses to abiotic stresses. However, the biological roles of MYB family members in rose (Rosa hybrida) are still unknown. Here, we reported the isolation and functional characterization of a novel rose RhMYB96 gene. RhMYB96 have a close relationship to Arabidopsis AtMYB96, including the presence of typical R2- and R3-domains at the N-terminal. Salt, dehydration, or ethylene signaling induce the expression of RhMYB96. RhMYB96 localized in the nucleus when expressed in Arabidopsis thaliana. We generated three independent transgenic Arabidopsis overexpressing RhMYB96 to explore the function of RhMYB96 in salt tolerance. Overexpression of RhMYB96 in Arabidopsis resulted in NaCl insensitivity during germination. Furthermore, RhMYB96-overexpressing seedlings under salt stress had longer primary root length and increased biomass. Specifically, the RhMYB96-overexpressed Arabidopsis plants displayed higher capability of scavenging the reactive oxygen levels H2O2 and O2− under salt stress. In addition, overexpressing RhMYB96 improved abscisic acid (ABA) sensitivity during germination. Expression of abiotic stresses and ABA-related genes was stronger in RhMYB96-overexpressing plants. These findings suggest that the RhMYB96 plays and regulates salt stress tolerance modulating an ABA-mediated pathway. Main Conclusion The novel rose RhMYB96 regulates salt stress through the modulation of an ABA-mediated pathway in Arabidopsis.
KeywordsRose RhMYB96 Regulator Salt ABA
Murashige and Skoog
open reading frame
quantitative reverse transcription PCR
This work was supported by the National Natural Science Foundation of China (Grant No. 31501798) and Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. BS2014SW032).
- Fang Q, Jiang T, Xu L, Liu H, Mao H, Wang X, Jiao B, Duan Y, Wang Q, Dong Q, Yang L, Tian G, Zhang C, Zhou Y, Liu X, Wang H, Fan D, Wang B, Luo K (2017) A salt-stress-regulator from the Poplar R2R3 MYB family integrates the regulation of lateral root emergence and ABA signaling to mediate salt stress tolerance in Arabidopsis. Plant Physiol Bioch 114:100–110Google Scholar
- Kotchoni SO, Kuhns C, Ditzer A, Kirch H-H, Bartels D (2006) Over-expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress. Plant Cell Environ 29:1033–1048CrossRefPubMedGoogle Scholar
- Nakashima K, Tran LP, Van Nguyen D, Fujita M, Maruyama K, Todaka D, Ito Y, Hayashi N, Shinozaki K, Yamaguchi-Shinozaki K (2007) Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice. Plant J 51:617–630CrossRefPubMedGoogle Scholar
- Parcy F, Giraudat J (1997) Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues. Plant J 11:693–702Google Scholar
- Shan H, Chen S, Jiang J, Chen F, Chen Y, Gu C, Li P, Song A, Zhu X, Gao H, Zhou G, Li T, Yang X (2012) Heterologous expression of the chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana. Mol Biotechnol 51:160–173CrossRefPubMedGoogle Scholar