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Plant Molecular Biology Reporter

, Volume 36, Issue 3, pp 406–417 | Cite as

The Novel Rose MYB Transcription Factor RhMYB96 Enhances Salt Tolerance in Transgenic Arabidopsis

  • Xinqiang Jiang
  • Shaocui Li
  • Aiqin Ding
  • Zhujun Zhang
  • Qing Hao
  • Kuiling Wang
  • Qingchao Liu
  • Qinghua Liu
Original Paper
  • 149 Downloads

Abstract

MYB transcription factors play diverse roles in plant development and responses to abiotic stresses. However, the biological roles of MYB family members in rose (Rosa hybrida) are still unknown. Here, we reported the isolation and functional characterization of a novel rose RhMYB96 gene. RhMYB96 have a close relationship to Arabidopsis AtMYB96, including the presence of typical R2- and R3-domains at the N-terminal. Salt, dehydration, or ethylene signaling induce the expression of RhMYB96. RhMYB96 localized in the nucleus when expressed in Arabidopsis thaliana. We generated three independent transgenic Arabidopsis overexpressing RhMYB96 to explore the function of RhMYB96 in salt tolerance. Overexpression of RhMYB96 in Arabidopsis resulted in NaCl insensitivity during germination. Furthermore, RhMYB96-overexpressing seedlings under salt stress had longer primary root length and increased biomass. Specifically, the RhMYB96-overexpressed Arabidopsis plants displayed higher capability of scavenging the reactive oxygen levels H2O2 and O2 under salt stress. In addition, overexpressing RhMYB96 improved abscisic acid (ABA) sensitivity during germination. Expression of abiotic stresses and ABA-related genes was stronger in RhMYB96-overexpressing plants. These findings suggest that the RhMYB96 plays and regulates salt stress tolerance modulating an ABA-mediated pathway. Main Conclusion The novel rose RhMYB96 regulates salt stress through the modulation of an ABA-mediated pathway in Arabidopsis.

Keywords

Rose RhMYB96 Regulator Salt ABA 

Abbreviations

ABA

abscisic acid

DAB

diaminobenzidine

H2O2

hydrogen peroxide

MDA

malondialdehyde

MS

Murashige and Skoog

NBT

nitroblue tetrazolium

ORF

open reading frame

O2

superoxide anion

qRT-PCR

quantitative reverse transcription PCR

TFs

transcription factors

WT

wild type

Notes

Funding Information

This work was supported by the National Natural Science Foundation of China (Grant No. 31501798) and Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province (No. BS2014SW032).

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xinqiang Jiang
    • 1
  • Shaocui Li
    • 1
  • Aiqin Ding
    • 1
  • Zhujun Zhang
    • 1
  • Qing Hao
    • 1
  • Kuiling Wang
    • 1
  • Qingchao Liu
    • 1
  • Qinghua Liu
    • 1
  1. 1.College of Landscape Architecture and ForestryQingdao Agricultural UniversityQingdaoChina

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