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Plant Molecular Biology Reporter

, Volume 36, Issue 3, pp 429–438 | Cite as

Genome-Wide Analysis of Lectin Receptor-Like Kinases in Tomato (Solanum lycopersicum) and Its Association with the Infection of Tomato Yellow Leaf Curl Virus

  • Tongmin Zhao
  • Jinyan Wang
  • Baolong Zhang
  • Xilin Hou
Original Paper

Abstract

Lectin receptor-like kinases (LecRLKs) are crucial factors in response to plant biotrophic pathogens. This study aimed to systemically analyze the effect of tomato LecRLKs on the responses to tomato yellow leaf curl virus (TYLCV) infection. A total of 93 tomato SlyLecRLK genes, including 69 G-type LecRLKs, 23 L-type LecRLKs, and one C-type LecRLK, were identified using genome-wide analysis and were clustered into three subfamilies using phylogenetic analysis. These SlyLecRLK genes were localized on the 12 tomato chromosomes with some gene duplication events. Transcriptomic analysis showed most of SlyLecRLKs were modulated by phytohormones and TYLCV infection. Gene ontology (GO) enrichment analysis showed all SlyLecRLK genes were associated with receptor protein kinase activity and protein modification. Quantitative RT-PCR (qRT-PCR) and gene interference validation showed SlyLecRLKs007, SlyLecRLKs036, and SlyLecRLKs044 genes were inhibited by TYLCV infection, and silencing of them were accompanied by accumulation of TYLCV DNA content. Subcellular localization of LecRLKs023 and LecRLKs044 on cell membranes was determined using a SlyLecRLK-containing A. tumefaciens strain GV3101. These results showed SlyLecRLK genes were key factors for TYLCV resistance, and also provided crucial information uncovering the protective effects of SlyLecRLKs on resisting TYLCV infection in tomato.

Keywords

Genome-wide analysis Tomato TYLCV Lectin receptor-like kinases 

Notes

Authors’ Contributions

Tongmin Zhao, Jinyan Wang, and Xilin Hou carried out the data mining, bioinformatics, gene expression analysis, and drafted the manuscript. Baolong Zhang and Jinyan Wang participated in the duplication analysis. Tongmin Zhao and Xilin Hou conceived and designed the study, and revised the manuscript. All authors read and approved the final manuscript.

Funding Information

This work was supported by the Fund for the Independent Innovation of Agricultural Sciences in Jiangsu Province (CX(12)1004, ZX(15)2003), National Natural Science Foundation of China (No. 31701918) and Natural Science Foundation of Jiangsu Province (BK20150543).

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no competing interests.

Supplementary material

11105_2018_1091_MOESM1_ESM.png (1.6 mb)
Figure S1 Gene structural analysis and identification of conserved motifs of 93 SlyLecRLKs. Gene Structure Display Server program with default settings were used for gene structure analysis. Conserved motifs in SlyLecRLKs were statistically identified using Multiple EM for Motif Elicitation. Different color blocks indicate the different motifs. Lines between motifs represent introns. G-, L- and C-type LecRLKs is indicated by circles, triangles and squares, respectively. (JPG 3004 kb)
11105_2018_1091_MOESM2_ESM.png (88 kb)
Figure S2 The correlation of expression levels as determined by RNA-Seq and qRT-PCR. (JPG 180 kb)
11105_2018_1091_MOESM3_ESM.xlsx (9 kb)
Table S1 (XLSX 9 kb)
11105_2018_1091_MOESM4_ESM.xlsx (66 kb)
Table S2 (XLSX 65 kb)
11105_2018_1091_MOESM5_ESM.xlsx (15 kb)
Table S3 (XLSX 15 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Tongmin Zhao
    • 1
    • 2
  • Jinyan Wang
    • 3
  • Baolong Zhang
    • 3
  • Xilin Hou
    • 1
  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable CropsJiangsu Academy of Agricultural SciencesNanjingChina
  3. 3.Provincial Key Laboratory of AgrobiologyJiangsu Academy of Agricultural SciencesNanjingChina

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