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Plant Molecular Biology Reporter

, Volume 35, Issue 6, pp 600–610 | Cite as

Miscanthus sp.: Genetic Diversity and Phylogeny in China

  • Chunxia Ge
  • Xiuming Liu
  • Shimin Liu
  • Jing Xu
  • Hongfei Li
  • Tengteng Cui
  • Yao Yao
  • Ming Chen
  • Weili Yu
  • Cuixia ChenEmail author
Original Paper

Abstract

Miscanthus genetic resources are widely distributed throughout China. However, genetic studies on Miscanthus lagged far behind other crops (e.g., sorghum, maize). To establish the comprehensive genetics knowledge of Miscnathus in China, here we report the genetic and phylogenetic diversity of 174 domestic Miscanthus accessions, along with an external Miscanthus × giganteus control. Cytological observations and flow cytometry analyses indicated that there were two major Miscanthus cytotypes in China: diploid (86.86%) and tetraploid (12.57%) without triploid. A total of 108 polymorphic loci generated from 25 SSR primers were used to evaluate the genetic variation. Large variations in genetic similarity coefficients (GSCs), ranging from 0.08 to 0.97 with a mean value of 0.39, were observed between these Miscanthus accessions. Our phylogenetic data revealed that these accessions were clustered into four main clades: M. section Miscanthus, M. section Diandranthus, M. section Triarrhena, and hybrids. The average percentage of polymorphic loci (P), gene diversity (H), and Shannon’s diversity index (I) among Miscanthus species are 70.93%, 0.22, and 0.34, respectively. These were consistent with the analysis of molecular variance (AMOVA) results, showing that 85% of genetic variation was found within clades. This study investigated the clear phylogenetic relationship of Miscanthus species in China, which will be valuable for further utilization of the germplasm in genetic improvement and hybrid breeding of Miscanthus.

Keywords

Miscanthus Cytotype Genetic diversity Molecular phylogeny SSR polymorphisms 

Notes

Acknowledgements

This work was supported by the National Natural Sciences Foundation of China (31271352 and 31071471) and the Department of Science and Technology of Shandong province (2013GNC11102). We thank Dr. Ji-Ping Zhao (USA) and Dr. Yanyan Wang (Associate Professor in University of Illinois, College of Medicine at Urbana-Champaign) for their English improvement and constructive comments on this manuscript.

Supplementary material

11105_2017_1048_MOESM1_ESM.xlsx (41 kb)
ESM 1 (XLSX 40 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Chunxia Ge
    • 1
  • Xiuming Liu
    • 1
  • Shimin Liu
    • 1
  • Jing Xu
    • 1
  • Hongfei Li
    • 1
  • Tengteng Cui
    • 1
  • Yao Yao
    • 1
  • Ming Chen
    • 1
  • Weili Yu
    • 2
  • Cuixia Chen
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Biology, College of AgronomyShandong Agricultural UniversityTai’ anChina
  2. 2.Taishan UniversityTai’ anChina

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