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Plant Molecular Biology Reporter

, Volume 34, Issue 1, pp 242–248 | Cite as

A Single Nucleotide Polymorphism of Nud Converts the Caryopsis Type of Barley (Hordeum Vulgare L.)

  • Shuiyang Yu
  • Hai Long
  • Guangbing Deng
  • Zhifen Pan
  • Junjun Liang
  • Xingquan Zeng
  • Yawei Tang
  • Nyima Tashi
  • Maoqun YuEmail author
Original Paper

Abstract

Whether the caryopsis of barley is naked or covered is controlled by the Nud gene encoding an ethylene response factor (ERF) family transcription factor (TF) of the AP2/ERF TF superfamily that is involved in the lipid biosynthesis pathway. In covered barley, lipids on the surface of caryopses act as a glue for their tight adhesion with hulls. Separation of hulls in naked barley is due to the absence of surface lipids on caryopses. A natural deletion event of Nud was found to be the determinant of naked caryopsis and had been proposed as the monophyletic origin of domesticated naked barley. In the present study, we re-sequenced the nud locus in 162 barleys, comprising 79 covered and 83 naked barleys varieties. Besides the normal deletion mutation of Nud contributing to naked caryopsis, we found a novel allele of Nud, designated as nud1.g, distinctly in three naked barleys collected from Tibet. nud1.g contains a non-synonymous SNP T643A when compared with the functional Nud gene. Genetic analysis indicated that the SNP T643A of nud1.g co-segregates with the naked phenotype. The nud1.g gene was expressed normally in the three naked barleys. Furthermore, in silico prediction of functionally conserved sites and 3D structures showed that the amino acid substitution (valine to aspartate) caused by SNP T643A may lead to a dramatic structural alteration of NUD that may result in loss of function. This study provides evidence of a new origin of the naked phenotype of domesticated barley in Tibet.

Keywords

Barley (Hordeum vulgare L.) Naked caryopsis Nud Single nucleotide polymorphism 

Notes

Acknowledgments

We thank Professor Lihua Chen, Qinghai Academy of Agriculture and Forestry Sciences for providing barley accessions with the name of WDMxxxx. This work was supported by the National Science and Technology Supporting Programs of China (2012BAD03B01).

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11105_2015_911_MOESM1_ESM.xls (36 kb)
ESM 1 (XLS 36 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Shuiyang Yu
    • 1
    • 2
  • Hai Long
    • 1
  • Guangbing Deng
    • 1
  • Zhifen Pan
    • 1
  • Junjun Liang
    • 1
  • Xingquan Zeng
    • 3
  • Yawei Tang
    • 3
  • Nyima Tashi
    • 3
  • Maoqun Yu
    • 1
    Email author
  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduPeople’s Republic of China
  2. 2.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  3. 3.Tibet Academy of Agriculture and Animal SciencesLhasaPeople’s Republic of China

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